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Comparative effect of fenofibrate on hepatic desaturases in wild-type and peroxisome proliferator-activated receptor α-deficient mice

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Lipids

Abstract

In this study is presented the effect of fenofibrate, a prototypical peroxisome proliferator of the fibrate class, on wild-type and peroxisome proliferator-activated receptor α (PPARα)-/-mouse liver FA profile, desaturase mRNA levels, and activities. We established that, following peroxisome proliferator exposure, the hepatic FA profile was greatly modified. These modifications in hepatic FA content required the expression of PPARα, as they are suppressed in transgenic mice deficient in this nuclear receptor. Following peroxisome proliferator exposure, Δ6- and Δ5-desaturase mRNA levels and activities were increased in wild-type but not in PPARα-deficient mouse liver. These results suggest the involvement of PPARα in the control of hepatic Δ6- and Δ5-desaturases in mice. Their roles in minimizing long-chain PUFA depletion in the liver during peroxisome proliferator exposure are discussed.

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Abbreviations

AOX:

peroxisomal acyl-CoA oxidase

CYP4A10:

cytochrome P450 4A10

PP:

peroxisome proliferator

PPARα:

peroxisome proliferator-activated receptor α

PPRE:

peroxisome proliferator responsive element

SCD:

stearoyl-CoA desaturase

TBS:

Tris-buffered saline

W-T:

wild-type

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Author information

Authors and Affiliations

  1. Laboratoire de Biochimie ENSAR-INRA, 65 Rue de Saint-Brieuc, 35042, Rennes, CS 84215, France

    Hervé Guillou, Sophie Jan, Sabine D’Andrea, Daniel Catheline, Vincent Rioux & Philippe Legrand

  2. Laboratoire de Pharmacologie et Toxicologie, INRA, 31931, Toulouse, France

    Pascal Martin, Alain Roulet & Thierry Pineau

  3. Université du Maine, 72000, Le Mans, France

    Sophie Jan

Authors
  1. Hervé Guillou
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  2. Pascal Martin
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  3. Sophie Jan
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  4. Sabine D’Andrea
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  5. Alain Roulet
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  6. Daniel Catheline
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  7. Vincent Rioux
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  8. Thierry Pineau
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  9. Philippe Legrand
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Correspondence to Philippe Legrand.

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Guillou, H., Martin, P., Jan, S. et al. Comparative effect of fenofibrate on hepatic desaturases in wild-type and peroxisome proliferator-activated receptor α-deficient mice. Lipids 37, 981–989 (2002). https://doi.org/10.1007/s11745-006-0990-3

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  • DOI: https://doi.org/10.1007/s11745-006-0990-3

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