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Dietary protein modulates the effect of CLA on lipid metabolism in rats

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Lipids

Abstract

The effect of the interaction of CLA and type of dietary protein on lipid metabolism was studied in male rats by feeding diets containing casein (CAS) or soy protein (SOY) as dietary protein and either linoleic acid (LA, a control FA) or graded levels of CLA at 0,0.1, 0.5, and 1.0% for 28 d. CLA reduced the weight of perirenal adipose tissue in a dose-dependent manner, but the magnitude of the reduction was greater when rats were fed SOY. Feeding SOY resulted in a significant reduction of the concentrations of serum total and HDL cholesterol, TG, glucose, and insulin irrespective of dietary CLA. The concentration of serum leptin tended to be lower on the SOY diet free of CLA than in the corresponding CAS diet, but it fell with an increasing dietary level of CLA in the CAS groups. In contrast, serum leptin tended to increase when CLA was added to SOY diets. The concentration of serum adiponectin was higher in the CAS than in the SOY groups, and it tended to increase in response to dietary CLA levels in the CAS-fed rats, whereas CLA showed no effect in SOY-fed rats. The activity of liver mitochondrial carnitine palmitoyltransferase was higher in the SOY than in the CAS groups, but it tended to increase with an increasing dietary level of CLA in both protein groups. Although the body fat-reducing activity of CLA was more effective when the protein source was SOY, rats fed CAS appeared to be more susceptible to CLA than in those fed SOY with respect to cytokines examined. These results suggest that the type of dietary protein may modify the antiobesity activity of CLA.

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Abbreviations

CAS:

casein

CPT:

carnitine palmitoyltransferase

GOT:

glutamic-oxaloacetic transaminase

GPT:

glutamic-pyruvic transaminase

LA:

linoleic acid

SOY:

soy protein

References

  1. Pariza, M.W., Park, Y., and Cook, M.E. (1999) Conjugated Linoleic Acid and the Control of Cancer and Obesity, Toxicol. Sci. 52, 107–110.

    PubMed  CAS  Google Scholar 

  2. Kritchevsky, D. (2000) Antimutagenic and Some Other Effects of Conjugated Linoleic Acid, Br. J. Nutr. 83, 459–465.

    PubMed  CAS  Google Scholar 

  3. Pariza, M.W., Park, Y., and Cook, M.E. (2001) The Biologically Active Isomers of Conjugated Linoleic Acid, Prog. Lipid Res. 40, 283–298.

    Article  PubMed  CAS  Google Scholar 

  4. Siems, W.G., Grune, T., Hasselwander, O., and Kramer, K. (2001) Conjugated Linoleic Acid, in Nutraceuticals in Health and Disease Prevention (Kramer K., Hoppe, P.P., and Packer, L., eds.), pp. 257–288, Marcel Dekker, New York.

    Google Scholar 

  5. Parodi, P.W. (2002) Conjugated Linoleic Acid, Food Aust. 54, 96–99.

    Google Scholar 

  6. Kelly, G.S. (2001) Conjugated Linoleic Acid: A review, Altern. Med. Rev. 6, 367–382.

    PubMed  CAS  Google Scholar 

  7. Ip, C., Chin, S.F., Scimeca, J.A., and Pariza, M.W. (1991) Mammary Cancer Prevention by Conjugated Dienoic Derivative of Linoleic Acid, Cancer Res. 51, 6118–6124.

    PubMed  CAS  Google Scholar 

  8. Terpstra, A.H.M. (2001) Differences Between Humans and Mice in Efficacy of the Body Fat-Lowering Effect of Conjugated Linoleic Acid: Role of Metabolic Rate, J. Nutr. 131, 2067–2068.

    PubMed  CAS  Google Scholar 

  9. Zambell, K.L., Keim, N.L., Van Loan, M.D., Gale, B., Benito, P., Kelley, D.S., and Nelson, G.J. (2000) Conjugated Linoleic Acid Supplementation in Humans: Effects on Body Composition and Energy Expenditure, Lipids 35, 777–782.

    Article  PubMed  CAS  Google Scholar 

  10. Mougios, V., Matsakas, A., Petridou, A., Ring, S., Sagredos, A., Melissopoulou, A., Tsigilis, N., and Nikolaidis, M. (2001) Effect of Supplementation with Conjugated Linoleic Acid on Human Serum Lipids and Body Fat, J. Nutr. Biochem. 12, 585–594.

    Article  PubMed  CAS  Google Scholar 

  11. Smedman, A., and Vessby B. (2001) Conjugated Linoleic Acid Supplementation in Humans—Metabolic Effects, Lipids 36, 773–781.

    Article  PubMed  CAS  Google Scholar 

  12. Thom, E., Wadstein, J., and Gudmundsen, O. (2001) Conjugated Linoleic Acid Reduces Body Fat in Healthy Exercising Humans, J. Int. Med. Res. 29, 392–396.

    PubMed  CAS  Google Scholar 

  13. Riserus, U., Arner, P., Brismar, K., and Vessby, B. (2002) Treatment with Dietary trans10,cis12 Conjugated Linoleic Acid Causes Isomer-Specific Insulin Resistance in Obese Men with the Metabolic Syndrome, Diabetes Care 25, 1516–1521.

    PubMed  CAS  Google Scholar 

  14. Stangl, G.I. (2000) High Dietary Levels of a Conjugated Linoleic Acid Mixture Alter Hepatic Glycerophospholipid Class Profile and Cholesterol-Carrying Serum Lipoprotein of Rats, J. Nutr. Biochem. 11, 184–191.

    Article  PubMed  CAS  Google Scholar 

  15. Yamasaki, M., Mansho, K., Ogino, Y., Kasai, M., Tachibana, H. and Yamada, K. (2000) Acute Reduction of Serum Leptin Level by Dietary Conjugated Linoleic Acid in Sprague-Dawley Rats, J. Nutr. Biochem. 11, 467–471.

    Article  PubMed  CAS  Google Scholar 

  16. Roche, H.M., Noone, E., Sewter, C., McBennett, S., Savage, D., Gibney, M.J., O'Rahilly, S., and Vidal-Puig, A.J. (2002) Isomer-Dependent Metabolic Effects of Conjugated Linoleic Acid: Insight from Molecular Markers Sterol Regulatory Element-Binding Protein-1c and LXRα, Diabetes. 51, 2037–2044.

    PubMed  CAS  Google Scholar 

  17. Clement, L., Poirier, H., Noit, I., Bocher, V., Guerre-Millo, M., Krief, S., Staels, B., and Besnard, P. (2002) Dietary trans10-, cis-12 Conjugated Linoleic Acid Induces Hyperinsulinemia and Fatty Liver in the Mouse, J. Lipid Res. 43, 1400–1409.

    Article  PubMed  CAS  Google Scholar 

  18. Sugano, M., Akahoshi, A., Koba, K., Tanaka, K., Okumura, T., Matsuyama, H., Goto, Y., Miyazaki, T., Murao, K., Yamasaki, M., et al. (2001) Dietary Manipulations of Body Fat-Reducing Potential of Conjugated Linoleic Acid in rats, Biosci. Biotechnol. Biochem. 65, 2535–2541.

    Article  PubMed  CAS  Google Scholar 

  19. Reeves, P.G., Nielsen, F.H., and Fahey, G.C. (1993) AIN-93 Purified Diets for Laboratory Rodents: Final Report of the American Institute of Nutrition ad hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet, J. Nutr. 123, 1939–1951.

    PubMed  CAS  Google Scholar 

  20. Bieber, L.L., Abraham, T., and Helmrath, T. (1972) A Rapid Spectrophotometric Assay for Carnitine Palmitoyltransferase, Anal. Biochem. 50, 509–518.

    Article  PubMed  CAS  Google Scholar 

  21. Volgarev, M.N., Vysotosky, V.G., Meshcheryakova, V.A., Yatsyshina, T.A., and Steinke, F.H., (1989) Evaluation of Isolated Soy Protein Foods in Weight Reduction with Obese Hypercholesterolemic and Normocholesterolemic Obese Individuals, Nutr. Rep. Int. 36, 61–72.

    Google Scholar 

  22. Anderson, J.W., Johnstone, B.M., and Cook-Newell, M.E. (1995) Meta-analysis of the Effects of Soy Protein Intake on Serum Lipids, N. Engl. J. Med. 333, 276–282.

    Article  PubMed  CAS  Google Scholar 

  23. Tonstad, S., Smerud, K., and Hoie, L. (2002) A Comparison of the Effects of 2 Doses of Soy Protein or Casein on Serum Lipids, Serum Lipoproteins, and Plasma Total Homocysteine in Hypercholesterolemic Subjects, Am. J. Clin. Nutr. 76, 78–84.

    PubMed  CAS  Google Scholar 

  24. DeLany, J.P., and West, D.B. (2000) Changes in Body Composition with Conjugated Linoleic Acid, J. Am. Coll. Nutr. 19, 487S-493S.

    PubMed  CAS  Google Scholar 

  25. Jones, P.A., Lea, L.J., and Pendlington, R.U. (1999) Investigation of the Potential of Conjugated Linoleic Acid (CLA) to Cause Peroxisome Proliferation in Rats, Food Chem Toxicol. 37, 1119–1125.

    Article  PubMed  CAS  Google Scholar 

  26. Nagasawa, A., Fukui, K., Funahashi, T., Maeda, N., Shimomura, I., Kihara, S., Waki, M., Takamatsu, K., and Matsuzawa, Y. (2002) Effects of Soy Protein Diet on the Expression of Adipose Genes and Plasma Adiponectin, Horm. Metab. Res. 34, 635–639.

    Article  PubMed  CAS  Google Scholar 

  27. Pajvani, U.B., and Scherer, P.E. (2003) Adiponectin: Systemic Contributor to Insulin Sensitivity, Curr. Diab. Rep. 3, 207–213.

    PubMed  Google Scholar 

  28. Tschritter, O., Fritsche, A., Thamer, C., Haap, M., Shirkavand, F., Rahe, S., Staiger, H., Maerker, E., Haring, H., and Stumvoll, M. (2003) Plasma Adiponectin Concentrations Predict Insulin Sensitivity of Both Glucose and Lipid Metabolism, Diabetes 52, 239–243.

    PubMed  CAS  Google Scholar 

  29. Akahoshi, A., Goto, Y., Murao, K., Miyazaki, T., Yamasaki, M., Nonaka, M., Yamada, K., and Sugano, M. (2002) Conjugated Linoleic Acid Reduces Body Fats and Cytokine Levels of Mice, Biosci. Biotechnol. Biochem. 66, 916–920.

    Article  PubMed  CAS  Google Scholar 

  30. Yannakoulia, M., Yiannakouris, N., Bluher, S., Matalas, A.L., Klimis-Zacas, D., and Mantzoros, C.S. (2003) Body Fat and Macronutrient Intake in Relation to Circulating Soluble Leptin Receptor. Free Leptin Index, Adiponectin, and Resistin Concentrations in Healthy Humans, J. Clin. Endocrinol. Metab. 88, 1730–1736.

    Article  PubMed  CAS  Google Scholar 

  31. Stiger, H., Tschritter, O., Machann, J., Thamer, C., Fritsche, A., Maerker, E., Schick, F., Haring, H.U., and Stomvoll, M. (2003) Relationship of Serum Adiponectin and Leptin Concentrations with Body Fat Distribution in Humans, Obes. Res. 11, 368–372.

    Article  Google Scholar 

  32. Chen, Y., Ogawa, H., Narita, H., Ohtoh, K., Yoshida, T., and Yoshikawa, Y. (2003) Ratio of Leptin to Adiponectin as an Obesity Index of Cynomologus Monkeys (Macaca fascicularis), Exp. Anim. 52, 137–143.

    Article  PubMed  CAS  Google Scholar 

  33. Gavrila, A., Peng, C.K., Chan, J.L., Mietus, J.E., Goldberger, A.L., and Mantzoros, C.S. (2003) Diurnal and Ultradian Dynamics of Serum Adiponectin in Healthy Men: Comparison with Leptin, Circulating Soluble Leptin Receptor, and Cortisol Patterns, J. Clin. Endocrinol. Metab. 88, 2838–2843.

    Article  PubMed  CAS  Google Scholar 

  34. Silha, J.V., Krsek, M., Skrha, J.V., Sucharda, P., Nyomba, B.L., and Murphy, L.J. (2003) Plasma Resistin, Adiponectin and Leptin Levels in Lean and Obese Subjects: Correlation with Insulin Resistance, Eur. J. Endocrinol. 149, 331–335.

    Article  PubMed  CAS  Google Scholar 

  35. Wolf, G. (2003) Adiponectin: A Regulator of Energy Homeostasis, Nutr. Rev. 61, 290–292.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Faculty of Nursing and Nutrition, Siebold University of Nagasaki, Nagayo, 851-2195, Nagasaki, Japan

    Kazunori Koba

  2. Laboratory of Food Chemistry, Department of Bioscience and Biotechnology, Graduate School Kyushu University, 812-8581, Kukuoka, Japan

    Masao Yamasaki

  3. Department of Research and Development, Rinoru Oils Mills Co., 104-0033, Tokyo, Japan

    Toshio Iwata, Yoshie Yamauchi & Kentaro Tsutsumi

  4. Faculty of Environmental and Symbiotic Sciences, Pretectural University of Kumamoto, 3-1-100 Tsukide, 862-8502, Kumamoto, Japan

    Asuka Akahoshi, Fumika Ichinose, Mai Kaneko, Asako Shimoda, Kikuko Nonaka & Michihiro Sugano

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  1. Asuka Akahoshi
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  2. Kazunori Koba
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Correspondence to Asuka Akahoshi.

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Akahoshi, A., Koba, K., Ichinose, F. et al. Dietary protein modulates the effect of CLA on lipid metabolism in rats. Lipids 39, 25–30 (2004). https://doi.org/10.1007/s11745-004-1197-3

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  • DOI: https://doi.org/10.1007/s11745-004-1197-3

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