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Chylomicron and VLDL TAG structures and postprandial lipid response induced by lard and modified lard

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Lipids

Abstract

Alterations in chylomicron and VLDL TAG and the magnitude of postprandial lipemia were studied in healthy volunteers after two meals of equal FA composition but different TAG-FA positional distribution. Molecular level information of individual lipoprotein TAG regioisomers was obtained with a tandem MS method. The incremental area under the response curve of VLDL TAG was large (P=0.021) after modified lard than after lard. In plasma TAG, the difference did not quite reach statistical significance (P=0.086). In general, there were less TAG with palmitic acid in the sn-2 position and more TAG with oleic acid in the sn-2 position in chylomicrons than in fat ingested. From 1.5 to 8 h postprandially, the proportion of individual chylomicron TAG was constant or influenced by TAG M.W. VLDL TAG regioisomerism was similar regardless of the positional distribution of fat ingested. Significant alterations were seen in VLDL TAG FA, in M.W. fractions, and in individual regioisomers with respect to time. The TAG sn-14∶0-18∶1-18∶1+sn-18∶1-18∶1-14∶0, sn-16∶0-16∶1-18∶1+sn-18∶1-16∶1-16∶0, and sn-16∶1-18∶1-18∶1+sn-18∶1-18∶1-16∶1 decreased (P<0.05); and sn-16∶0-16∶0-18∶2+sn-18∶2-16∶0-16∶0, sn-16∶0-16∶0-18∶1+sn-18∶1-16∶0-16∶0, sn-16∶0-18∶1-16∶0, and sn-16∶0-18∶1-18∶2+sn-18∶2-18∶1-16∶0 increased (P<0.05) after both meals. In conclusion, positional distribution of TAG FA was found to affect postprandial lipid metabolism in healthy normolipidemic subjects.

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Abbreviations

ACN:

acyl carbon number

apo:

apolipoprotein

DB:

double bond

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Author information

Authors and Affiliations

  1. Department of Clinical Nutrition, University of Kuopio, Finland

    Ursula S. Schwab & Hannu M. Mykkänen

  2. Department of Biochemistry and Food Chemistry, University of Turku, FIN-20014, Turku, Finland

    Kaisa M. Yli-Jokipii, Raija L. Tahvonen, Juha-Pekka Kurvinen & Heikki P. T. Kallio

Authors
  1. Kaisa M. Yli-Jokipii
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  2. Ursula S. Schwab
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  3. Raija L. Tahvonen
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  4. Juha-Pekka Kurvinen
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  5. Hannu M. Mykkänen
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  6. Heikki P. T. Kallio
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Correspondence to Kaisa M. Yli-Jokipii.

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Yli-Jokipii, K.M., Schwab, U.S., Tahvonen, R.L. et al. Chylomicron and VLDL TAG structures and postprandial lipid response induced by lard and modified lard. Lipids 38, 693–703 (2003). https://doi.org/10.1007/s11745-003-1117-6

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  • DOI: https://doi.org/10.1007/s11745-003-1117-6

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