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Analysis of diastereomeric DAG naphthylethylurethanes by normal-phase HPLC with on-line electrospray MS

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Lipids

Abstract

Normal-phase HPLC resolution of sn-1,2(2,3)- and x-1,3-DAG generated by partial Grignard degradation from natural TAG was carried out with both (R)-(−) and (S)-(+)-1-(1-naphthyl)ethylurethane derivatives. The diastereomeric sn-1,2- and sn-2,3-DAG derivatives were resolved using two Supelcosil LC-Si (5 μm, 25 cm × 4.6 mm i.d.) columns in series and an isocratic elution with 0.37% isopropanol in hexane at a flow rate of 0.7 mL/min. The DAG were detected by UV absorption at 280 nm and were identified by electrospray ionization MS in the positive ion mode following postcolumn addition of chloroform/methanol/30% ammonium hydroxide (75∶24.5∶0.5, by vol) at 0.6 mL/min. Application of the method to a stereospecific analysis of the molecular species of TAG of rat VLDL showed that the TAG composition of VLDL circulating under basal conditions differs markedly from that of VLDL secreted by the liver during inhibition of serum lipases. The inhibition of serum lipases resulted in a significant proportional decrease in 16∶0 and PUFA and an increase in 18∶0 and oligoenoic FA in the sn-1-position, whereas the FA compositions in the sn-2- and sn-3-positions were much less affected.

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Abbreviations

CapEx:

capillary exit voltage

DNPU:

dinitrophenylurethane

HPLC/ESI-MS:

HPLC with on-line electrospray ionization MS

NEU:

1-(1-naphthyl)ethylurethane

14∶0:

myristic acid

16∶0:

palmitic acid

18∶0:

stearic acid

18∶1:

oleic acid

18∶2:

linoleic acid

18∶3:

linolenic acid

20∶4:

arachidonic acid

20∶5:

eicosapentaenoic acid

22∶4:

docosatetraenoic acid

22∶5:

docosapentaenoic acid

22∶6:

docosahexaenoic acid

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Authors and Affiliations

  1. Banting and Best Department of Medical Research, University of Toronto, M5G 1L6, Toronto, Ontario, Canada

    J. J. Ågren & A. Kuksis

  2. Department of Physiology, University of Kuopio, P.O. Box 1627, FIN-70211, Kuopio, Finland

    J. J. Ågren

Authors
  1. J. J. Ågren
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  2. A. Kuksis
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Correspondence to J. J. Ågren.

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Ågren, J.J., Kuksis, A. Analysis of diastereomeric DAG naphthylethylurethanes by normal-phase HPLC with on-line electrospray MS. Lipids 37, 613–619 (2002). https://doi.org/10.1007/s11745-002-0940-0

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  • DOI: https://doi.org/10.1007/s11745-002-0940-0

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