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Effects of dietary defatted squid on cholesterol metabolism and hepatic lipogenesis in rats

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Lipids

Abstract

Male Sprague-Dawley rats were fed a cholesterol-free (Exp. 1) or cholesterol-supplemented (Exp. 2) diet containing 20% casein (control group) or 15% defatted squid and 5% casein (defatted squid group), as protein, for 14 d. Serum and hepatic cholesterol concentrations were lower in rats fed defatted squid than in those fed casein in both cholesterol-free (−20%, P<0.05 and −15%, P<0.05, respectively) and cholesterol-supplemented (−25%, P<0.05 and −15%, P<0.05, respectively) diets. Hepatic triglyceride concentration was lower in the defatted squid than in the control groups in both cholesterol-free (−51%, P<0.05) and cholesterol-supplemented diets (−38%, P<0.01). The activities of cytosolic fatty acid synthase and the NADPH-generating enzymes, malic enzyme and glucose-6-phosphate dehydrogenase, in the liver were lower in the defatted squid than in the control groups in both cholesterol-free (−21%, P<0.01, −33%, P<0.05, and −33%, P<0.01, respectively) and cholesterol-supplemented diets (−34%, P<0.05, −57%, P<0.05, and −67%, P<0.05, respectively). The activity of mitochondrial carnitine palmitoyltransferase in the liver was comparable between the control and defatted squid groups. The activity of Mg2+-dependent phosphatidate phosphohydrolase in the liver cytosol was lower in the defatted squid (−9%, P<0.05) than in the control groups only in the cholesterol-free diet. Fecal excretion of total steroids was stimulated by the feeding of defatted squid in both cholesterol-free (+77%, P<0.005) and cholesterol-supplemented diets (+29%, P<0.01). These results suggest that the nonlipid fraction of squid exerts a hypocholesterolemic effect by increasing the excretion of total steroids in feces. The fraction also induces a triglyceride-lowering activity in the liver by decreasing hepatic lipogenesis.

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Abbreviations

CPT:

carnitine palmitoyltransferase

DHA:

docosahexaenoic acid

EPA:

eicosapentaenoic acid

FAS:

fatty acid synthase

G6PDH:

glucose-6-phosphate dehydrogenase

HDL:

high density lipoprotein

PAP:

phosphatidate phosphohydrolase

PUFA:

polyunsaturated fatty acids

VLDL:

very low density lipoprotein

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Authors and Affiliations

  1. Laboratory of Nutrition Chemistry, Faculty of Agriculture, Graduate School Kyushu University, 812-8581, Fukuoka, Japan

    Ikuo Ikeda & Katsumi Imaizumi

  2. Division of Food and Nutrition, Nakamura Junior College, 814-0198, Fukuoka, Japan

    Hiroko Yoshida

  3. Laboratory of Nutrition Chemistry, Siebold University of Nagasaki, 822 Yoshimuta Goh, Nagayo Cho, 851-2195, Nishisonogi Gun, Nagasaki Pref., Japan

    Kazunari Tanaka

Authors
  1. Kazunari Tanaka
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  2. Ikuo Ikeda
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  4. Katsumi Imaizumi
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Correspondence to Kazunari Tanaka.

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Tanaka, K., Ikeda, I., Yoshida, H. et al. Effects of dietary defatted squid on cholesterol metabolism and hepatic lipogenesis in rats. Lipids 36, 461–466 (2001). https://doi.org/10.1007/s11745-001-0743-3

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  • DOI: https://doi.org/10.1007/s11745-001-0743-3

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