Abstract
Diet and fatty acid metabolism interact in yet unknown ways to modulate membrane fatty acid composition and certain cellular functions. For example, dietary precursors or metabolic products of n-3 fatty acid metabolism differ in their ability to modify specific membrane components. In the present study, the effect of dietary 22∶6n−3 or its metabolic precursor, 18∶3n−3, on the selective accumulation of 22∶6n−3 by heart was investigated. The mass and fatty acid compositions of individual phospholipids (PL) in heart and liver were quantified in mice fed either 22∶6n−3 (from crocodile oil) or 18∶3n−3 (from soybean oil) for 13 wk. This study was conducted to determine if the selective accumulation of 22∶6n−3 in heart was due to the incorporation of 22∶6n−3 into cardiolipin (CL), a PL most prevalent in heart and known to accumulate 22∶6n−3. Although heart was significantly enriched with 22∶6n−3 relative to liver, the accumulation of 22∶6n−3 by CL in heart could not quantitatively account for this difference. CL from heart did accumulate 22∶6n−3, but only in mice fed preformed 22∶6n−3. Diets rich in non-22∶6n−3 fatty acids result in a fatty acid composition of phosphatidylcholine (PC) in heart that is unusually enriched with 22∶6n−3. In this study, the mass of PC in heart was positively correlated with the enrichment of 22∶6n−3 into PC. The increased mass of PC was coincident with a decrease in the mass of phosphatidylethanolamine, suggesting that 22∶6n−3 induced PC synthesis by increasing phosphatidylethanolamine-N-methyltransferase activity in the heart.
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Abbreviations
- CE:
-
cholesterol ester
- CL:
-
cardiolipin
- CRO:
-
crocodile oil
- FFA:
-
free fatty acid
- PC:
-
phosphatidylcholine
- PE:
-
phosphatidylethanolamine
- PEMT:
-
phosphatidylethanolamine-N-methyltransferase
- PL:
-
phospholipids
- SO:
-
soybean oil
- TG:
-
triacylglyceride
- TLC:
-
thin-layer chromatography
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Watkins, S.M., Lin, T.Y., Davis, R.M. et al. Unique phospholipid metabolism in mouse heart in response to dietary docosahexaenoic or α-linolenic acids. Lipids 36, 247–254 (2001). https://doi.org/10.1007/s11745-001-0714-8
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DOI: https://doi.org/10.1007/s11745-001-0714-8