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Low density lipoprotein receptor mRNA in rat liver is affected by resistant starch of beans

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Lipids

Abstract

The effects of resistant starches of beans on serum cholesterol and hepatic low density lipoprotein (LDL) receptor mRNA in rats were investigated. Rats were fed a cholesterol-free diet with 150 g/kg corn starch (CS), 150g/kg adzuki (Vigna angularis) starch (AS), 150 g/kg kintoki (Phaseolus vulgaris, variety) starch (KS), or 150 g/kg tebou (P. vulgaris, variety) starch (TS) for 4 wk. There were no significant differences in body weight among groups through the experimental period. The liver weight in the CS group was 1.1–1.2 times higher than that in the AS, KS, and TS groups. The cecum weight in the TS was 1.4 times higher than that in the CS group, and the cecal pH in the CS group was significantly higher than in the other groups. The serum total cholesterol, very low density lipoprotein + intermediate density lipoprotein +LDL-cholesterol and high density lipoprotein (HDL)-cholesterol concentrations in the bean starch groups were significantly lower than those in the CS group through the feeding period. The total cholesterol/HDL-cholesterol ratio in the bean starch groups was also significantly lower than that in the CS group at the end of the 4-wk feeding period. The hepatic cholesterol concentration in the TS group was significantly higher than in the CS group at the end of the 4-wk feeding period. The relative quantity of hepatic apo B mRNA in the AS group was 1.2 times higher than that in the CS group, and the hepatic LDL receptor mRNA levels in the AS and TS groups were 1.8–2.0 times higher than that in the CS group. The results of this study demonstrate that AS, KS, and TS lowered the serum total cholesterol level by enhancing the hepatic LDL receptor mRNA level.

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Abbreviations

Apo:

apolipoprotein

AS:

adzuki starch

CS:

corn starch

DIG:

digoxigenin

GAPDH:

glyceraldehyde-3-phosphate dehydrogenase

GLC:

gas-liquid chromatography

HDL:

high density lipoprotein

IDL:

intermediate density lipoprotein

KS:

kintoki starch

LDL:

low density lipoprotein

PCR:

polymerase chain reaction

RT:

reverse transcription

SCFA:

short-chain fatty acid

TG:

triglyceride

TS:

tebou starch

VLDL:

very low density lipoprotein

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Authors and Affiliations

  1. Department of Bioresource Science, Obihiro University of Agriculture and Veterinary Medicine, 080-8555, Obihiro, Hokkaido, Japan

    Michihiro Fukushima, Tetsu Ohashi, Michiyuki Kojima & Masuo Nakano

  2. Hokkaido Tokachi Area Regional Food Processing Technology Center, Obihiro, 080-2462, Hokkaido, Japan

    Kiyoshi Ohba & Hideki Shimizu

  3. Laboratory of Food Biochemistry, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, 060-8589, Hokkaido, Japan

    Kei Sonoyama

Authors
  1. Michihiro Fukushima
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  2. Tetsu Ohashi
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  3. Michiyuki Kojima
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  4. Kiyoshi Ohba
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  5. Hideki Shimizu
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  6. Kei Sonoyama
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  7. Masuo Nakano
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Correspondence to Michihiro Fukushima.

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Fukushima, M., Ohashi, T., Kojima, M. et al. Low density lipoprotein receptor mRNA in rat liver is affected by resistant starch of beans. Lipids 36, 129–134 (2001). https://doi.org/10.1007/s11745-001-0698-4

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  • DOI: https://doi.org/10.1007/s11745-001-0698-4

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