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Cacao liquor polyphenols reduce oxidative stress without maintaining α-tocopherol levels in rats fed a vitamin E-deficient diet

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Lipids

Abstract

The effect of crude polyphenols (CLP) from cacao liquor on vitamin F-deficient rats was examined. The CLP fraction contained 49.8% antioxidative polyphenols such as catechins and their oligomers. Supplementation of the vitamin E-deficient diet with CLP for 7 wk did not prevent the decrease in α-tocopherol levels in the liver, kidney, heart, brain, and plasma. The lipid peroxide levels in these tissues increased in the group fed the vitamin F-deficient diet compared with the control group. However, these changes were inhibited in a dose-dependent manner as a result of supplementation of the vitamin E-deficient diet with 0.25, 0.5, or 1.0% CLP. The lipid peroxide levels in plasma increased in the group fed the vitamin E-deficient diet. This change tended to be suppressed as a result of supplementation of the diet with CLP, but the difference was not significant. There was no evidence of absorption and distribution of CLP to the tissues; however, CLP intake resulted in a decrease in oxidative stress without maintaining vitamin E levels in the plasma and the tissues.

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Abbreviations

CLI:

cacao liquor crude polyphenols

EC:

epicatechin

PMC:

2,2,5,7,8-pentamethyl-6-chromarol

TBARS:

thiobarbituric acid-reactive substances

VE:

vitamin E

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Authors and Affiliations

  1. Graduate School of Bioagricultural Science, Nagoya University, Chikusa, 464-8601, Nagoya, Japan

    Toshihiko Osawa

  2. Functional Foods R&D Laboratory, Meiji Seika Kaisha Ltd., 5-3-1, 350-0289, Chiyoda, Sakado, Saitama, Japan

    Megumi Yamagishi, Naomi Osakabe & Toshio Takizawa

Authors
  1. Megumi Yamagishi
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  2. Naomi Osakabe
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  3. Toshio Takizawa
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  4. Toshihiko Osawa
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Correspondence to Naomi Osakabe.

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Yamagishi, M., Osakabe, N., Takizawa, T. et al. Cacao liquor polyphenols reduce oxidative stress without maintaining α-tocopherol levels in rats fed a vitamin E-deficient diet. Lipids 36, 67–71 (2001). https://doi.org/10.1007/s11745-001-0669-9

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  • DOI: https://doi.org/10.1007/s11745-001-0669-9

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