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Effect of n−3 polyunsaturated fatty acid supplementation on lipid peroxidation of rat organs

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Lipids

Abstract

The present study was undertaken in order to reexamine the effect of n−3 polyunsaturated fatty acid (PUFA)-rich diet supplementation on lipid peroxidation and vitamin E status of rat organs. Male Wistar rats were fed a diet containing safflower or fish oil at 50 g/kg diet and an equal amount of vitamin E at 59 mg/kg diet (1.18 g/kg oil; and 1.5 g/kg PUFA in safflower oil diet, and 4.3 g/kg PUFA in fish oil diet) for 6 wk. Fatty acid composition of total lipids of brain, liver, heart, and lung of rats fed fish oil was rich in n−3 PUFA, whereas that of each organ of rats fed safflower oil was rich in n−6 PUFA. The vitamin E levels in liver, stomach, and testis of the fish oil diet group were slightly lower than those of the safflower oil diet group, but the levels in brain, heart, lung, kidney, and spleen were not different between the two diet groups. The levels of phospholipid hydroperoxides were determined by the high-performance liquid chromatography-chemiluminescence method and the levels of thiobarbituric acid-reactive substances (TBARS) were determined at pH 3.5 in the presence of butylated hydroxytoluene with or without EDTA. Levels of phospholipid hydroperoxides and TBARS in the brain, liver, heart, lung, kidney, spleen, stomach and testis of the fish oil diet group were similar to those of the safflower oil diet group. The results indicate that high fish oil intake does not induce increased levels of phospholipid hydroperoxides and TBARS in rat organs.

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Abbreviations

BHT:

butylated hydroxytoluene

HPLC:

high-performance liquid chromatography

PC:

phosphatidylcholine

COOH:

PC hydroperoxide

PE:

phosphatidylethanolamine

PEOOH:

PE hydroperoxide

PI:

peroxidizability index

PUFA:

polyunsaturated fatty acids

TBA:

thiobarbituric acid

TBARS:

TBA-reactive substances

UI:

unsaturation index

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Authors and Affiliations

  1. Advanced Science and Technology Research Center, Kyushu University, Kasuga, 816-8580, Fukuoka, Japan

    Masao Suzuki

  2. School of Pharmacy, Tokyo University of Pharmacy and Life Science, 432-1 Horinouchi, Hachioji, 192-0392, Tokyo, Japan

    Ken Ando, Kunihide Nagata, Rie Yoshida & Kiyomi Kikugawa

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  1. Ken Ando
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  2. Kunihide Nagata
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  3. Rie Yoshida
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  4. Kiyomi Kikugawa
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  5. Masao Suzuki
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Correspondence to Kiyomi Kikugawa.

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Ando, K., Nagata, K., Yoshida, R. et al. Effect of n−3 polyunsaturated fatty acid supplementation on lipid peroxidation of rat organs. Lipids 35, 401–407 (2000). https://doi.org/10.1007/s11745-000-538-6

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  • DOI: https://doi.org/10.1007/s11745-000-538-6

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