Abstract
Hairy roots of Tagetes patula were initiated by infecting the seedlings with Agrobacterium rhizogenes. The hairy roots were grown in liquid medium MS (Murashige and Skoog 1962) supplemented with sucrose. The roots were treated with three different elicitors obtained from mycelial culture of Aspergillus niger, Rhizopus oligosporus and Pencillium notatum. Accumulation of biomass and the production of thiophenes were studied over a period of six weeks in culture. The HPLC separation profile of the thiophenes indicated the presence of several structurally different thiophenes. α-terthienyl being predominent. Maximum production of thiophene was recorded at the end of the fourth week in culture with a content of 0.138 % (w/w on dry weight basis). Treatment of hairy roots with mycelial extract of Aspergillus niger (1.5 % v/v) elicited an increase in thiophene content by 1.6 folds over the control.
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Abbreviations
- HPLC:
-
High performance liquid chromatography
- FW:
-
Fresh weight
- V/V:
-
Volume per volume
- W/V:
-
Weight per volume
References
Bhagyalakshmi N. and Ravishankar G.A. 1998. Natural compounds from cultured hairy roots. (Eds.) I.A. Khan and A. Khanum. Role of biotechnology in medicinal and Aromatic plants. Ukaaz Publications, Hyderabad, India, pp. 165–182.
Bohlmann F., Burkhardt T. and Zdero C. (Eds.), 1973. Naturally occurring acetylenes. Academic Press Inc. (London) Ltd., pp.340–463.
croes A.F., Van den Berg A.J.R., Bosveld M., Breteler H. and Wullems C.J. 1989. Thiophene accumulation in relation to morphology in roots of Tagetes patula: Effects of auxin and transformation by Agrobacterium. Planta. 79: 43–50.
DiCosmo F. and Misawa M. 1985. Eliciting secondary metabolism in plant cell cultures. Trends Biotechnol. 3: 318–322.
Ketel D.H. 1987. Distribution and accumulation of thiophenes in plants and calli of different Tagetes species. J. Exp. Bot. 38: 322–330.
Murashige T. and Skoog F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol.Plant. 15: 473–497.
Norton R.A., Finlayson and Towers G.H.N. 1985. Thiophene production by crown galls and callus tissues of Tagetes patula. Phytochemistry, 24: 719–722.
Rajendran L., Suvarnalatha G., Ravishankar G.A. and Venkataraman L.V. 1994. Enhancement of anthocyanin production in callus cultures of Daucus carota, L. under the influence of fungal elicitors. Appl. Microbiol. Biotechnol., 42: 227–231.
Sudhakar J.T., Ravishankar G.A. and Venkataraman L.V. 1991. Elicitation of capsaicin production in freely suspended cells and immobilized cell cultures of Capsicum frutescens Mill. Food Biotechnol. 5: 197–205.
Trypsteen M., Van Iijsebettens M., Van Severan R. and Van Montagu 1991. Agrobacterium rhizogenes-mediated transformation of Echinacea purpurea. Plant Cell Reports. 10: 85–89.
Van Latum E.B.J. and Gerrits R. 1991. Bio-Pesticides in developing countries: Prospects and Research Priorities. ACTS Press, Nairobi, Kenya.
Zdero C. 1985. Naturally occurring thiophenes. In: Chemistry of heterocyclic compounds. Eds. A. Weissberger and E.C. Taylor. Part one: Thiophene and its derivatives (Ed. S. Gronowitz), John Wiley and Sons Inc., New York, pp. 261–323.
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Rajasekaran, T., Madhusudan, R. & Ravishankar, G.A. Elicitation of thiophene production by cultured hairy roots of Tagetes patula . Acta Physiol Plant 21, 243–247 (1999). https://doi.org/10.1007/s11738-999-0038-4
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DOI: https://doi.org/10.1007/s11738-999-0038-4