Abstract
The effect of calcium-deprivation on growth and the production of cardenolides in two undifferentiated cell lines of Digitalis thapsi maintained under three different light regimes (16 h photoperiod, darkness, or continuous light) was investigated. Growth was stimulated by continuous light in both cell lines cultured in complete medium. The light regime did not affect cardenolide accumulation in the cells of the hypocotyl-derived line; by contrast, continuous light or darkness increased the production in the leaf-derived line. The elimination of calcium favoured cardenolide production independently of the origin of the suspensions and the light regime, this beneficial effect being predominantly manifested under continuous light.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- BA:
-
N6-benzyladenine
- EGTA:
-
ethyleneglycol-bis-(ß-aminoethyl ether)-N,N′-tetraacetic acid
- FW:
-
fresh weight
- MS:
-
Murashige and Skoog (1962)
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Cacho, M., Morán, M., Corchete, P. et al. Effect of calcium restriction on cardenolide accumulation in two cell lines of Digitalis thapsi grown under different light regimes. Acta Physiol Plant 21, 335–340 (1999). https://doi.org/10.1007/s11738-999-0003-2
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DOI: https://doi.org/10.1007/s11738-999-0003-2