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Characterization of Capsicum annuum L. leaf and root antimicrobial peptides: antimicrobial activity against phytopathogenic microorganisms

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Abstract

This study aimed to detect and characterize antimicrobial proteins, especially antimicrobial peptides (AMPs) from leaves and roots of Capsicum annuum and to evaluate their inhibitory activities against different phytopathogenic fungi and the bacterium Xanthomonas euvesicatoria. Two methodologies were used for the extraction of peptides from leaves and roots of C. annuum: acid and ethanolic extraction. Extracts were subjected to reversed-phase chromatography on HPLC. The extraction and purification procedures were analysed by uni- and bi-dimensional electrophoresis in tricine gels. Our results show that alcoholic and acid extracts from both tissues can inhibit the growth of the phytopathogenics fungi C. lindemuthianum and C. gloeosporioides. The acid extracts from both tissues are active against X. euvesicatoria and only leaf extracts displayed specific inhibitory activity towards trypsin and α-amylase activity. The data compiled here aim to contribute to establish the multiplicity of potential uses of plant AMPs for the control of pests and pathogens of agricultural relevance.

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Abbreviations

AMPs:

Antimicrobial peptides

LTP:

Lipid-transfer proteins

BAPNA:

N-Benzoyl-dl-arginyl-p-nitroanilide

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Acknowledgements

We acknowledge the financial support of the Brazilian agencies CNPq (305495/2017-8), FAPERJ (E-26/202.735/2016), and CAPES through the CAPES/Toxinology project.

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Authors and Affiliations

  1. Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, 28105620, Campos dos Goytacazes, RJ, Brazil

    Lídia da Silva Pereira, Viviane Veiga do Nascimento, Suzanna de Fátima Ferreira Ribeiro, Katia Valevski Sales Fernandes, André de Oliveira Carvalho, Cíntia dos Santos Bento & Valdirene Moreira Gomes

  2. Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brazil

    Rosana Rodrigues & Cláudia Pombo Sudré

  3. Laboratório de Toxinas Vegetais, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Brazil

    Ilka Maria Vasconcelos

  4. Centro de Ciências da Saúde, Universidade Federal de Roraima, Boa Vista, RR, Brazil

    Umberto Zottich

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  1. Lídia da Silva Pereira
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  2. Viviane Veiga do Nascimento
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  3. Suzanna de Fátima Ferreira Ribeiro
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  8. Cíntia dos Santos Bento
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  11. Valdirene Moreira Gomes
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Correspondence to Valdirene Moreira Gomes.

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Communicated by Z.-L. Zhang.

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da Silva Pereira, L., do Nascimento, V.V., de Fátima Ferreira Ribeiro, S. et al. Characterization of Capsicum annuum L. leaf and root antimicrobial peptides: antimicrobial activity against phytopathogenic microorganisms. Acta Physiol Plant 40, 107 (2018). https://doi.org/10.1007/s11738-018-2685-9

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  • DOI: https://doi.org/10.1007/s11738-018-2685-9

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