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Cadmium disrupts apoplastic ascorbate redox status in barley root tips

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Abstract

Using a short-term Cd treatment (5–30 min), we analysed the effect of Cd on apoplastic ascorbate redox status and their regeneration during the recovery period in barley root tips. Root growth inhibition induced by 15 μM Cd was detectable after 5 min of exposure and increased in a time-dependent manner up to 15 min of exposure. High 30 μM Cd concentration completely inhibited root growth during the first 6 h after short-term treatment. In parallel with Cd-induced root growth inhibition, a rapid decrease of apoplastic ascorbate dehydroascorbate ratio was observed immediately after short-term treatments. During the recovery from 15 μM Cd short-term treatment, apoplastic ascorbate was rapidly regenerated to the control level in the first root segment containing meristem and elongation zone. In contrast to 15 μM Cd treatment, in 30 μM Cd-treated roots apoplastic ascorbate level was sustained at a significantly lower level compared to control roots. We confirmed that a decrease of apoplastic ascorbate/dehydroascorbate ratio in the elongation zone was associated with root growth inhibition or arrest.

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Abbreviations

ASC:

Ascorbate

DHA:

Dehydroascorbate

DTT:

Dithiothreitol

G6PDH:

Glucose-6-phosphate dehydrogenase

ROS:

Reactive oxygen species

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Acknowledgments

We wish to thank Margita Vašková for excellent technical assistance. This work was supported by the Grant agency VEGA, project No. 2/0050/10.

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Authors and Affiliations

  1. Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 9, 84523, Bratislava, Slovak Republic

    Beáta Bočová, Igor Mistrík, Ján Pavlovkin & Ladislav Tamás

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  1. Beáta Bočová
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  2. Igor Mistrík
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  3. Ján Pavlovkin
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  4. Ladislav Tamás
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Correspondence to Ladislav Tamás.

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Communicated by G. Klobus.

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Bočová, B., Mistrík, I., Pavlovkin, J. et al. Cadmium disrupts apoplastic ascorbate redox status in barley root tips. Acta Physiol Plant 34, 2297–2302 (2012). https://doi.org/10.1007/s11738-012-1030-y

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  • DOI: https://doi.org/10.1007/s11738-012-1030-y

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