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Biotechnological approaches for cultivation and enhancement of secondary metabolites in Arnica montana L.

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Abstract

Arnica montana L. (Asteraceae) is a valuable medicinal plant with anti-inflammatory and cicatrizing properties attributed to the sesquiterpene lactones, flavonoids and essential oil produced in the flower heads. In many European countries, the populations of A. montana are close to extinction in their natural habitats because of uncontrolled eradication and indiscriminate collection of the plants. Various approaches for in vitro propagation of the species and also, biosynthesis of secondary metabolites in tissue and cell cultures are assessed in the current review. Special attention is paid to the biological activity and chemical composition of compounds produced by the species as well as the opportunities of in vitro methods to isolate high-productive clones. The influence of different factors on the micropropagation, callusogenesis, genetic transformation and identification of certain biologically active substances is discussed in detail. By the reference to the available issues we concluded that biotechnology applied to A. montana cultivation may improved the plant preservation and increased the production of sesquiterpene lactones and other secondary metabolites.

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References

  • Albert A, Sareedenchai V, Heller W, Seidlitz HK, Zidorn Ch (2009) Temperature is the key to altitudinal variation of phenolics in Arnica montana L. cv. ARBO. Oecologia 160(1):1–8. doi:10.1007/s00442-009-1277-1

    Article  PubMed  Google Scholar 

  • Assyov B, Petrova A, Dimitrov D, Vassilev R (ed) (2006) Conspectus of the Bulgarian vascular flora. Distribution maps and floristic elements. Bulgarian—Swiss Biodiversity Conservation Program, 2nd edn. BSBCP, Sofia, p 452

  • Bensaddek L, Villarreal ML, Fliniaux M (2008) Induction and growth of hairy roots for the production of medicinal compounds. Electron J Integr Biosci 3(1):2–9

    Google Scholar 

  • Bezzi A, Ghidini G (1988) Preliminary trails on the cultivation of Arnica montana L. in the southern Alps and in the northern Apennines. Annali dell’Instituto Sperimentale per l’Assestamento Forestale e per l’Apicoltura 9:305–320

    Google Scholar 

  • Bomme U (1993) Cultivation of Arnica montana is now possible. Bodenkultur und Pflanzenbau 5(13):3–11

    Google Scholar 

  • Bomme U, Mittermeier M, Regenhardt I (1995) Results to develop a procedure for field-cultivation of Arnica montana L. 1 and 2. Mitteilung. Drogenreport 8(12):5–10

    Google Scholar 

  • Bourgaud F, Gravot A, Milsei S, Gontier E (2001) Production of plant secondary metabolites: a historical perspective. Plant Sci 161:839–851. doi:10.1016/S0168-9452(01)00490-3

    Article  CAS  Google Scholar 

  • Butiuc-Keul AL, Deliu C (2001) Clonal propagation of Arnica montana L., a medicinal plant. In vitro Cell Dev Biol Plant 37:581–585. doi:10.1079/IVP2001222

    Google Scholar 

  • Butiuc-Keul AL, Suteu A, Keul M, Deliu C (2002) The role of natural extract on the in vitro mulitiplication of Arnica montana L. Contrib Bot 37:183–189

    Google Scholar 

  • Cassells AC, Walsh C, Belin M, Cambornac M, Robin JR, Lubrano C (1999) Establishment of a plantation from micropropagated Arnica chamissonis a pharmaceutical substitute for the endangered A. montana. Plant Cell Tiss Org 56(2):139–144. doi:10.1023/A:1006224215503

    Google Scholar 

  • Conchou O, Nichterlein K, Vömel A (1992) Shoot tip culture of Arnica montana for micropropagation. Planta Med 58(1):73–76. doi:10.1055/s-2006-961393

    Article  PubMed  CAS  Google Scholar 

  • Daniel G, Bomme U (1991) Use of in vitro culture techniques in the breeding of Arnica (Arnica montana L.). Bayerisches Landwirtsch Jahrbuch 68:249–253

    Google Scholar 

  • Delabays N, Mange N (1991) Culture of Arnica montana L.: the agronomy and plant protection. Rev Suisse Vitic Arboric Hortic 23(5):313–319

    Google Scholar 

  • Douglas JA, Smallfield BM, Burgess EJ, Perry NB, Anderson RE, Douglas MH, Glennie VL (2004) Sesquiterpene lactones in Arnica montana: a rapid analytical method and the effects of flower maturity and simulated mechanical harvesting on quality and yield. Planta Med 70:166–170. doi:10.1055/s-2004-815495

    Article  PubMed  CAS  Google Scholar 

  • Ellenberger A (1998) Assuming responsibility for a protected plant: WELEDA’s endeavor to secure the firm’s supply of Arnica montana. In: Proceedings of the first international symposium on the conservation of medicinal plants in trade in Europe. Royal Botanic Gardens, Kew, 22–23 June, pp 127–130

  • Evstatieva L (2008) Arnica chamissonis Less—a new medicinal plants for Bulgaria. In: 5th conference on medicinal and aromatic plants of Southeast European countries, Brno Czech Republic, pp 10–14

  • Flores HE, Medina-Bolivar F (1995) Root culture and plant natural products:”unearthing” the hidden half of plant metabolism. Plant Tiss Cult Biotechnol 1:59–74

    Google Scholar 

  • Galambosi B (2004) Introduction of Arnica montana L in Finland. Z Arzn Gew Pfl 9 Jg 4:174–179

    Google Scholar 

  • Galambosi B, Sz-Galambosi Z, Svoboda KP, Deans SG (1998) Flower yield and antioxidant properties of Arnica montana L. grown in Finland. Drogenreport 11(19):10–13

    Google Scholar 

  • Ganzera M, Egger C, Zidorn C, Stuppner H (2008) Quantitative analysis of flavonoids and phenolic acids in Arnica montana L. by micellar electrokinetic capillary chromatography. Anal Chim Acta 614:196–200. doi:10.1016/j.aca.2008.03.023

    Article  PubMed  CAS  Google Scholar 

  • Garcia-Pineres AJ, Castro V, Mora G, Schmidt TJ, Strunck E, Pahl H, Merfort I (2001) Cysteine 38 in p65/NF—kappa B plays a crucial role in DNA binding inhibition by sesquiterpene lactones. J Biol Chem 276:39713–39720. doi:10.1074/jbc.M101985200

    Article  PubMed  CAS  Google Scholar 

  • Georgiev M, Pavlov AI, Bley T (2007) Hairy root type plant in vitro systems as sources of bioactive substances. Appl Microbiol Biotechnol 74(6):1175–1185. doi:10.1007/s00253-007-0856-5

    Article  PubMed  CAS  Google Scholar 

  • Güntzel U, Seidel F, Kating H (1967) Studies on the contents of Arnica-species. I. Content on essential oil in the flowers of different Arnica-species. Planta Med 15(2):205–214. doi:10.1055/s-0028-1099974

    Article  PubMed  Google Scholar 

  • Iauk L, Lo Bue AM, Milazzo I, Rapisarda A, Blandino G (2003) Antibacterial activity of medicinal plant extracts against periodontopathic bacteria. Phytother Res 17(6):599–604. doi:10.1002/ptr.1188

    Article  PubMed  CAS  Google Scholar 

  • Judžentienė A, Būdienė J (2009) Analysis of the chemical composition of flower essential oils from Arnica montana of Lithuanian origin. Chemija 20:190–194

    Google Scholar 

  • Kalynyak PP, Trofymyak TB, Komisarenko MF, Blyum YB (1995) Production of callus lines for plants from Arnica genus and analysis of their biosynthetic activity. Dopov Natsional Akad Nauk Ukrayiny 9:107–110 (in Ukraine)

    Google Scholar 

  • Karuppusamy S (2009) A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. J Med Plants Res 3(13):1222–1239

    CAS  Google Scholar 

  • Kating H, Rinn W, Willuhn G (1970) Studies on the substance of species of Arnica. 3. Fatty acids in etheric oils of the flowers of various species of Arnica. Planta Med 18(2):130–146. doi:10.1055/s-0028-1099758

    Article  PubMed  CAS  Google Scholar 

  • Kennedy JF, Stevenson DL, White CA, Lombard A, Buffa M (1988) Analysis of the oligosaccharides from the roots of Arnica montana L., Artemisia absinthium L., and Artemisia dracuncula L. Carbohydr Polymers 9(4):277–285. doi:10.1016/0144-8617(88)90046-X

  • Klass CA, Wagner G, Laufer S, Sosa S, Della Loggia R, Bomme U, Pahl HL, Merfort I (2002) Studies on the anti-inflammatory activity of phytopharmaceuticals prepared from Arnica flowers. Planta Med 68:385–391. doi:10.1055/s-2002-32067

    Article  Google Scholar 

  • Kohlmünzer S (ed) (2000) Farmakognozja. Pharmacognosy. Guide for pharmacy students. PZWL, Warszawa, p 669 (in Polish)

  • Kos O, Lindenmeyer MT, Tubaro A, Sosa S, Merfort I (2005) New sesquiterpene lactones from arnica tincture prepared from fresh flowerheads of Arnica montana. Planta Med 71:1044–1052. doi:10.1055/s-2005-871284

    Article  PubMed  CAS  Google Scholar 

  • Lange D (1998) Europe’s medicinal and aromatic plants: their use, trade and conservation. TRAFFIC International, Cambridge, pp 53–54

    Google Scholar 

  • Lê CL (1994) In vitro multiplication of Arnica montana L. Revue Suisse de Viticulture, d’Arboriculture et d’Horticulture 26:391–395

    Google Scholar 

  • Lê CL (1998) In vitro clonal multiplication of Arnica montana L. In: Proc. Plant Biotehnology as a tool for the exploitation of mountain lands, Turin, Italy, 25–27 May 1997. Acta Hortic 457:195–203

  • Lê CL (2000) Factors influencing the in vitro proliferation of Arnica montana L. In: COST 843, WG1: developmental biology of regeneration, Ist Meeting, 12–15 Oct 2000, Geisenheim, Germany, pp 29–30

  • Leven W, Willuhn G (1986) Spectrophotometric determination of sesquiterpenelactone S1 in Arnicae Flos Dab 9 with m-dinitrobenzene. Planta Med 52:537–538

    Article  Google Scholar 

  • Lopez-Anton N, Hermann C, Murillo R, Merfort I, Wanner G, Vollmar AM, Dirsch VM (2007) Sesquiterpene lactones induce distinct forms of cell death that modulate human monocyte-derived macrophage responses. Apoptosis 12:141–153. doi:10.1007/s10495-006-0331-2

    Article  PubMed  CAS  Google Scholar 

  • Lyss G, Schmidt TJ, Merfort I, Pahl HL (1997) Helenalin, an anti-inflammatory sesquiterpene lactone from Arnica, selectively inhibits transcription factor NF-kappa-B. Biol Chem 378:951–961. doi:10.1515/bchm.1997.378.9.951

    Article  PubMed  CAS  Google Scholar 

  • Maguire B (1943) A monograph of the genus Arnica (Senecioneae, Compositae). Brittonia 4:386–510

    Article  Google Scholar 

  • Malarz J, Stojakowska A, Dohnal B, Kisiel W (1993) Helenalin acetate in in vitro propagated plants of Arnica montana. Planta Med 59(1):51–53. doi:10.1055/s-2006-959603

    Article  PubMed  CAS  Google Scholar 

  • Merfort I (1992) Caffeoylquinic acids from flowers of Arnica montana and Arnica chamissonis. Phytochemistry (Oxford) 31:2111–2113. doi:10.1016/0031-9422(92)80373-M

  • Merfort I (2007) Arnika. New insights into an old medical plant. Internist Praxis 48(2):369–376

    Google Scholar 

  • Merfort I, Wendisch D (1985) Flavonoids from Arnica montana and Arnica chamissonis Planta Med 51(2):136–138. doi:10.1055/s-2007-969428

  • Merfort I, Wendisch D (1987) Flavonoid glycosides from Arnica montana and Arnica chamissonis. Planta Med 53(5):434–437. doi:10.1055/s-2006-962766

    Article  PubMed  CAS  Google Scholar 

  • Merfort I, Wendisch D (1988) Flavonoid glucuronides from the flowers of Arnica montana. Planta Med 54(3):247–250. doi:10.1055/s-2006-962419

    Google Scholar 

  • Merfort I, Wendisch D (1992) New flavonoid glycosides from Arnicae flos DAB 9. Planta Med 58(4):355–357. doi:10.1055/s-2006-961484

    Article  PubMed  CAS  Google Scholar 

  • Mulabagal V, Tsay H (2004) Plant cell cultures—an alternative and efficient source for the production of biologically important secondary metabolites. Int J Appl Sci Eng 2:29–48

    Google Scholar 

  • Nichterlein K (1995) Arnica montana (Mountain Arnica): in vitro culture and the production of sesquiterpene lactones and other metabolites. In: Bajaj YPS (ed) biotechnology in agriculture and forestry, medicinal and aromatic plants VIII, vol 33. Springer, Berlin, pp 47–61

  • Paßreiter CM, Willuhn G, Röder E (1992) Tussilagine and Isotussilagine: two pyrrolizidine alkaloids in the genus Arnica. Planta Med 58(6):556–557. doi:10.1055/s-2006-961549

    Article  PubMed  Google Scholar 

  • Perry NB, Burgess EJ, Guitián MAR, Franco RR, Mosquera EL, Smallfield BM, Joyce NI, Littlejohn RP (2009) Sesquiterpene Lactones in Arnica montana: helenalin and dihydrohelenalin chemotypes in Spain. Planta Med 75(6):660–666. doi:10.1055/s-0029-1185362

    Article  PubMed  CAS  Google Scholar 

  • Petriha RO, Masnuk YT (2008) Callusogenesis of Arnica montana L. in culture in vitro.Visnik National Univ 609:151–155

    Google Scholar 

  • Petrova M, Tasheva K, Zagorska N, Evstatieva L (2005) In vitro propagation of Arnica montana L. CR Acad Bulg Sci 58(1):67–72

    CAS  Google Scholar 

  • Petrova M, Zayova E, Vlahova M (2008) Induction of callus cultures in Arnica montana L. Genet Breed 37(3–4):37–44

    Google Scholar 

  • Petrova M, Zayova E, Yankova E, Baldzhiev G (2011) Plant regeneration from callus culture of Arnica montana. Rom Biotechnol Lett 16(1):92–97

    CAS  Google Scholar 

  • Radanović D, Marcović T, Antić-Mladenović S, Pljevlakušić D, Ristić M, Krivokuća- Dokić D (2007) Yield and quality of Arnica (Arnica montana and Arnica chamissonis var. foliosa) cultivated in Serbia. In: 1st international scientific conference on medicinal, aromatic and spice plants. Nitra, Slovakia, pp 157–161

  • Ramachandra Rao S, Ravishankar GA (2002) Plant tissue cultures; chemical factories of secondary metabolites. Biotechnol Adv 20:101–153. doi:10.1016/S0734-9750(02)00007-1

    Article  CAS  Google Scholar 

  • Rey C (1984) First results on cultivation of medicinal and aromatic plants in Switzerland. Acta Hortic 144:183–191

    Google Scholar 

  • Reyes-Salas O, Rangel-Ordoez L, Manzanilla-Cano JA, Barcel-Quintal MH, Dosal-Gmez MA (2002) Voltammetric determination of caffeic acid in Arnica Montana. Analytical Lett 35(6):971–984. doi:10.1081/AL-120004549

    Google Scholar 

  • Rhodes MJC, Robins RJ, Hamill JD, Parr AJ, Hilton MG, Walton NJ (1990) Properties of transformed root cultures. In: Charlwood BV, Rhodes MJC (eds) Secondary products from plant tissue culture. Oxford University Press, Oxford, pp 201–225

  • Rossetti V, Lombard A, Sancin P, Buffa M, Di Stefano R (1984) Characterization of Arnica montana dried roots. Int J Crude Drug Res 22:53–60

    CAS  Google Scholar 

  • Santer JO, Stevenson R (1962) Arnidiol and faradiol. J Org Chem 27:3204–3208

    Article  CAS  Google Scholar 

  • Santos MD, Almeida MC, Lopes NP, de Souza GE (2006) Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid. Biol Pharm Bull 29(11):2236–2240. doi:10.1248/bpb.29.2236

    Article  PubMed  Google Scholar 

  • Schmidt TJ, Paßreiter CM, Wendisch D, Willuhn G (1992) First diterpens from Arnica. Planta Med 58(Suppl 1):A713

    Article  Google Scholar 

  • Schmidt TJ, Bomme U, Alfermann AW (1998) Sesquterpene lactone content in leaves of in vitro and field cultivated Arnica montana. Planta Med 64(3):268–270. doi:10.1055/s-2006-957423

    Article  PubMed  CAS  Google Scholar 

  • Schmidt TJ, Stausberg S, von Raison J, Berner M, Willuhn G (2006) Lignans from Arnica species. Nat Prod Res 20:443–453. doi:10.1080/14786410500218591

    Article  PubMed  CAS  Google Scholar 

  • Schulte KE, Rücker G, Reithmayr K (1969) Certain constituents of Arnica chamissonis and other Arnica species. Lloydia 32(3):360–368

    Google Scholar 

  • Seeber H, Abraham H, Stupper H (1997) Culture experiments in South Tyrol with Arnica montana L. Drogenreport 10(16):10–16

    Google Scholar 

  • Seemann A, Wallner T, Poschlod P, Heilmann J (2010) Variation of sesquiterpene lactone contents in different Arnica montana populations: influence of ecological parameters. Planta Med 76(8):837–842. doi:10.1055/s-0029-1240797

    Google Scholar 

  • Spitaler R, Schlorhaufer PD, Ellmerer EP, Merfort I, Bortenschlager S, Stuppner H, Zidorn C (2006) Altitudinal variation of secondary metabolite profiles in flowering heads of Arnica montana cv. ARBO. Phytochemistry (Amsterdam) 67:409–417.doi:10.1016/j.phytochem.2005.11.018

  • Spitaler R, Winkler A, Lins I, Yanar S, Stuppner H, Zidorn CH (2008) Altitudinal variation of phenolic contents in flowering heads of Arnica montana cv. ARBO: a 3-year comparison. J Chem Ecol 34(3):369–375. doi:10.1007/s10886-007-9407-x

    Google Scholar 

  • Stafford A (1991) Natural products and metabolites from plants and plant tissue cultures. In: Stafford A, Warren G (eds) Plant Cell Tiss Org, Open University Press, Buckingham, pp 124–162

  • Staneva J, Denkova P, Todorova M, Evstatieva L (2011) Quantitative analysis of sesqiuterpene lactones in extract of Arnica montana L. by 1HNMR spectroscopy. J Pharmaceut Biomed Anal.54:94–99. doi:org/10.1016/j.jpba.2010.08.018

    Google Scholar 

  • Sugier D (2008) Trials with Arnica montana and Arnica chamissonis var. foliosa cultivation in Poland. In: 5th conference on medicinal and aromatic plants of southeast European countries (5th CMAPSEEC), Proceedings of scientific paper, 2-5.09.2008 Brno Czech Republic, pp 21–27

  • Tornhamre S, Schmidt TJ, Näsman-Glaser B, Ericsson I, Lindgren JA (2001) Inhibitory effects of helenalin and related compounds on 5-lipoxygenase and leukotriene C(4) synthase in human blood cells. Biochem Pharmacol 62(7):903–911. doi:10.1016/S0006-2952(01),00729-8

    Article  PubMed  CAS  Google Scholar 

  • Vanhaelen M (1973) Identification of carotenoids in Arnica Montana. Planta Med 23(4):308–311. doi:10.1055/s-0028-1099449

    Article  PubMed  CAS  Google Scholar 

  • Wagner S, Merfort I (2007) Skin penetration behaviour of sesquiterpene lactones from different Arnica preparations using a validated GC-MSD method. J Pharm Biomed Anal 43:32–38. doi:10.1016/j.jpba.2006.06.008

    Google Scholar 

  • Weremczuk-Jezyna I, Wysokiñska H (2000) Micropropagation of Arnica montana L. and sesquterpene lactones in regenerated plants. Biotechnologia 4:118–122

    Google Scholar 

  • Weremczuk-Jeżyna I, Wysokińska H (2004) Culture of transformed roots of Arnica montana—isolation of secondary metabolites. Biotechnologia 2:54–60

    Google Scholar 

  • Weremczuk-Jeżyna I, Kisiel W, Wysokińska H (2006) Thymol derivatives from hairy roots of Arnica montana. Plant Cell Rep 25(9):993–996. doi:10.1007/s00299-006-0157-y

    Article  PubMed  Google Scholar 

  • Weremczuk-Jeżyna I, Kalemba D, Wysokińska H (2011) Constituents of the essential oil from hairy roots and plant roots of Arnica montana. J Essent Oil Res 23:91–97

    Google Scholar 

  • White CA, Kennedy JF, Lombard A, Rossetti V (1985) Analysis of a homologous series of non-reducing oligosaccharides from Arnica montana L Roots. Brit Polym J 17:327–329. doi:10.1002/pi.4980170401

    Article  CAS  Google Scholar 

  • Willuhn G (1972a) Studies on the contents of Arnica species. V. Content and variations in content of volatile oils in the various organs of Arnica species. Planta Med 21(3):221–245. doi:10.1055/s-0028-1099547

    Article  PubMed  CAS  Google Scholar 

  • Willuhn G (1972b) Studies on the components of Arnica species. VI. Characterization and preparative separation of volatile oils from roots, rhizoma, leaves and flowerheads of various arnica species. Planta Med 21(4):329–342. doi:10.1055/s-0028-1099561

    Article  PubMed  CAS  Google Scholar 

  • Willuhn G (1989) Arnikablüten In: Wichtl M (ed) Teedrogen und Phyto-pharmaka, 2nd edn. Wissenschaftliche Verlagsgesellschaft GmbH, Stuttgart, pp 65–69

  • Willuhn G (1991) Arnica montana L. Portrait of a medicinal plant Arnica montana L. Pharm Ztg 136:2453–2468

    Google Scholar 

  • Willuhn G, Leven W (1991) On the qualitative and quantitative analysis of the sesquiterpene lactones from Arnica flowers DAB 9. Pharm Ztg Wiss 136(1):32–39

    Google Scholar 

  • Willuhn G, Rottger PM, Matthiesen U (1983) Helenalin and 11,13-dihydrohelenalin esters from the flowers of Arnica montana. Planta Med 49(12):226–231. doi:10.1055/s-2007-969856

    Article  PubMed  CAS  Google Scholar 

  • Willuhn G, Junior I, Wendisch D (1986) Desmethoxy-encecalin and thymolderivative from Arnica sachalinensis. Planta Med 5:349–351

    Article  PubMed  Google Scholar 

  • Willuhn G, Leven W, Luley C (1994) Arnica flowers German Pharmacopeia 10 (DAB 10): investigation of the qualitative and quantitative variability of sesquiterpene lactone contents of official Arnica crude drugs. Dstch Apotheker Ztg 134:4077–4085

    Google Scholar 

  • Winand C, Ramaut JL, Caspar T (1986) Multiplication vegetative par culture in vitro d’Arnica montana L. J Pharm Belg 41:147–150

    Google Scholar 

  • Woerdenbag HJ, Merfort I, Passreiter CM, Schmidt TJ, Willuhn G, Van Uden W, Pras N, Kampinga HH, Konings AWT (1994) Cytotoxicity of flavonoids and sesquiterpene lactones from Arnica species against the GLC-4 and the COLO 320 cell lines. Planta Med 60:434–437. doi:10.1055/s-2006-959526

    Article  PubMed  CAS  Google Scholar 

  • Wysokinska H, Chmiel A (2006) Secondary metabolites production in cultures of transformed plant organs. Biotechnologia 4:124–135

    Google Scholar 

  • Willuhn G (1998) Arnica flowers: pharmacology, toxicology and analysis of the sesquiterpene lactones-their main active substances. In: Lawson L. D., Bauer R. (ed.): Phytomedicines of Europe, ACS Symp. Ser 691 Am. Chem. Soc. 691:118–132 doi: 10.1021/bk-1998-0691.ch010

  • Zapartan M, Deliu C (2001) Studies of in vitro regeneration and multiplication of Arnica montana L. (Asteraceae). Contrib Bot 36:131–135

    Google Scholar 

  • Zayova E, Petrova M, Vitkova A, Yurukova P (2010) Effect of auxins on in vitro rhizogenesis and subsequent ex vitro acclimatization of Arnica montana. In: Biotechnology, Series F, Supplementary volume, Proceeding of the 3rd International Symposium “New Researches in Biotechnology” SimpBTH2010, pp 258–264

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  1. Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    Mariya Petrova, Ely Zayova & Roumiana Vassilevska-Ivanova

  2. AgroBioInstitute, 8 Dragan Tzankov Blvd., 1164, Sofia, Bulgaria

    Mariana Vlahova

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Petrova, M., Zayova, E., Vassilevska-Ivanova, R. et al. Biotechnological approaches for cultivation and enhancement of secondary metabolites in Arnica montana L.. Acta Physiol Plant 34, 1597–1606 (2012). https://doi.org/10.1007/s11738-012-0987-x

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