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Variation of phenolic composition and biological activities in Limoniastrum monopetalum L. organs

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Abstract

Changes in phenolic composition and biological activities were investigated in different Limoniastrum monopetalum L. organs. For that, 80% aqueous acetone extracts were used to estimate total phenolic contents and their antioxidant activities were evaluated using DPPH· and O ·−2 radical scavenging activities and reducing power. The efficiency of organ extracts was tested against human pathogen strains. Ultimately, acid hydrolysis of all organs was subjected to RP-HPLC for phenolic identification. Results showed that flower extracts exhibited the highest polyphenol (65.42 GAE/g DW) and flavonoid (35.36 CE/g DW) contents. Stems were enriched in condensed tannin content (21.4 mg CE/g DW) and displayed the best antiradical activities and the highest reducing power. Besides, stem and gall extracts showed the highest efficiency against pathogenic bacteria as compared with those of flower. Concerning the antifungal test, a slight activity was found in gall extracts. The RP-HPLC showed a difference in phenolic compounds that varied as function of organ. In fact, the major phenolic compound varied as function of organ. Results suggest that L. monopetalum could be a promising source of biomolecules for therapeutic and nutraceutical industries and the difference between organs may be related to their physiological role.

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Abbreviations

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

IC50 :

Inhibition concentration at 50%

EC50 :

Effective concentration at which the absorbance was 0.5

ATCC:

American type culture collection

NCIMB:

National Collection of Industrial Marine and Food Bacteria

PMS:

Phenazine methosulfate

NADH:

Nicotinamide adenine dinucleotide

NBT:

Nitro-tetrazolium blue chloride

BHT:

Butylated hydroxytoluene

GAE:

Gallic acid equivalents

CE:

Catechin equivalents

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Acknowledgments

This work was supported by the Tunisian Ministry of Higher Education, Research and Technology (LR02CB02) and by the Tunisian-French ‘‘Comité Mixte de Coopération Universitaire” (CMCU) network # 08G0917.

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Authors and Affiliations

  1. Laboratoire Des Plantes Extrêmophiles, Centre de Biotechnologie de Borj Cédria, BP 901, 2050, Hammam-Lif, Tunisia

    Najla Trabelsi, Hanen Falleh, Ines Jallali, Alia Ben Daly, Abderrazek Smaoui, Chedly Abdelly & Riadh Ksouri

  2. Laboratoire d’Analyses Et de Contrôle Des Polluants Chimiques Et Microbiologiques de l’Environnement, 15 Faculté de Pharmacie, Rue Avicenne, 5000, Monastir, Tunisia

    Hafedh Hajlaoui

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  1. Najla Trabelsi
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  2. Hanen Falleh
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  3. Ines Jallali
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Correspondence to Najla Trabelsi.

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Communicated by Z.-L. Zhang.

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Trabelsi, N., Falleh, H., Jallali, I. et al. Variation of phenolic composition and biological activities in Limoniastrum monopetalum L. organs. Acta Physiol Plant 34, 87–96 (2012). https://doi.org/10.1007/s11738-011-0807-8

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  • DOI: https://doi.org/10.1007/s11738-011-0807-8

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