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A rapid in vitro propagation of red sanders (Pterocarpus santalinus L.) using shoot tip explants

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Abstract

An efficient protocol has been developed for the in vitro propagation of Pterocarpus santalinus L. using shoot tip explants which is a valuable woody medicinal plant. Various parts of this plant are pharmaceutically used for the treatment of different diseases. Multiple shoots were induced from shoot tip explants derived from 20 days old in vivo germinated seedlings on 1:1 ratio of sand and soil after treating with gibberellic acid (GA3). The highest frequency for shoot regeneration (83.3%) with maximum number of shoot buds (11) per explant was obtained on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l of 6-benzylaminopurine (BAP) along with 0.1 mg/l of thidiazuron (TDZ) after 45 days of culture. A proliferating shoot culture was established by repeatedly subculturing the original shoot tip explants on fresh medium after each harvest of the newly formed shoots. Sixty percent of the shoots produced roots were transferred to rooting medium containing MS salts and 0.1 mg/l indole-3-butyric acid (IBA) after 30 days. About 73.33% of the in vitro raised plantlets were established successfully in earthen pots. Random amplified polymorphic DNA (RAPD)-based DNA fingerprinting profiles were generated for the first time using shoot tip explants of this species and confirmed that there was no genetic variability. This protocol might be helpful for the mass multiplication of P. santalinus in the future.

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Abbreviations

BAP:

6-benzylaminopurine

GA3 :

Gibberellic acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

KN:

Kinetin

MS:

Murashige and Skoog

NAA:

α-Naphthalene acetic acid

TDZ:

Thidiazuron

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Acknowledgments

The authors are grateful to the University Grants Commission, New Delhi, India, for providing financial assistance (Sanction No. F.31-238/2005 (SR) dated 05.04.2006).

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Authors and Affiliations

  1. Department of Plant Biology and Biotechnology, School of Life Sciences, Loyola College, Chennai, 600 034, Tamil Nadu, India

    P. Agastian

  2. Plant Biotechnology Division, Entomology Research Institute, Loyola College, Chennai, 600 034, Tamil Nadu, India

    K. Balaraju & S. Ignacimuthu

  3. National Academy of Agricultural Science (NAAS), RDA, Suwon, 441-707, South Korea

    K. Balaraju & Kyungseok Park

Authors
  1. K. Balaraju
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  2. P. Agastian
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  3. S. Ignacimuthu
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  4. Kyungseok Park
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Correspondence to K. Balaraju.

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Communicated by E. Lojkowska.

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Balaraju, K., Agastian, P., Ignacimuthu, S. et al. A rapid in vitro propagation of red sanders (Pterocarpus santalinus L.) using shoot tip explants. Acta Physiol Plant 33, 2501–2510 (2011). https://doi.org/10.1007/s11738-011-0795-8

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  • DOI: https://doi.org/10.1007/s11738-011-0795-8

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