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Characterization of p-hydroxybenzaldehyde dehydrogenase, the final enzyme of p-hydroxybenzoic acid biosynthesis in hairy roots of Daucus carota

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Abstract

Hairy root cultures of Daucus carota respond to methyl-jasmonate treatment with enhanced accumulation of p-hydroxybenzoic acid. The final C1-side chain of this compound is shaped by p-hydroxybenzaldehyde dehydrogenase (HBD) that catalyzes the formation of p-hydroxybenzoic acid from p-hydroxybenzaldehyde in the presence of NAD+. HBD was biochemically characterized from cell-free hairy root extracts of D. carota. The preferred substrate for HBD was p-hydroxybenzaldehyde. The apparent K m values were 54.8 and 74.4 μM for p-hydroxybenzaldehyde and NAD+, respectively. Divalent metal cations did not significantly affect enzyme activity.

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Fig. 1
Fig. 2

Abbreviations

DTT:

Dithiothreitol

HBD:

p-Hydroxybenzaldehyde dehydrogenase

MJ:

Methyl-jasmonate

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Acknowledgments

This work was supported by a research grant [No. 38(1201)/08/EMR-II to A. Mitra] from the Council of Scientific and Industrial Research (CSIR), India. D. Sircar acknowledges CSIR and German Academic Exchange Service (DAAD) for the award of a senior research fellowship (NET) and a short-term fellowship, respectively.

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Authors and Affiliations

  1. Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721 302, India

    Debabrata Sircar, Chiranjit Mukherjee & Adinpunya Mitra

  2. Institute of Pharmaceutical Biology, Technische Universität Braunschweig, Mendelssohnstraße 1, 38106, Brunswick, Germany

    Debabrata Sircar, Till Beuerle & Ludger Beerhues

Authors
  1. Debabrata Sircar
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  2. Chiranjit Mukherjee
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  3. Till Beuerle
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  4. Ludger Beerhues
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  5. Adinpunya Mitra
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Corresponding author

Correspondence to Adinpunya Mitra.

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Communicated by S. Lewak.

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Sircar, D., Mukherjee, C., Beuerle, T. et al. Characterization of p-hydroxybenzaldehyde dehydrogenase, the final enzyme of p-hydroxybenzoic acid biosynthesis in hairy roots of Daucus carota . Acta Physiol Plant 33, 2019–2024 (2011). https://doi.org/10.1007/s11738-011-0723-y

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  • DOI: https://doi.org/10.1007/s11738-011-0723-y

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