Skip to main content

Advertisement

SpringerLink
Log in

Effects of low nitrogen and drought stresses on proline synthesis of Jatropha curcas seedling

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

Proline is one of the most important osmoregulatory solutes subjected to osmotic stresses. In this study, low nitrogen supply suppressed the dry biomass, leaf area, and proline biosynthesis of the seedlings of the energy plant Jatropha curcas, which could grow in poor, dry soil. Low-nitrogen stress induced JcP5CS mRNA expression and decreased the activity of P5CS enzyme and the content of free proline in leaves of J. curcas seedlings. When the seedlings grown in low-nitrogen conditions were suddenly exposed to PEG-6000 (−1.6 MPa) stress, the expression of JcP5CS gene was highly induced, and both the activity of P5CS and the content of free proline increased and maintained at high levels to mitigate the impact of drought stresses. This may be one of the reasons why J. curcas could adapt to poor and drought conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Achten WMJ, Mathhijs E, Verchot L, Singh VP, Aerts R, Muys B (2007) Jatropha biodiesel fueling sustainability? Biofuels Bioprod Biorefining 1:283–291

    Article  CAS  Google Scholar 

  • Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216

    Article  CAS  Google Scholar 

  • Bandurska H, Stroiński A (2003) ABA and proline accumulation in leaves and roots of wild (Hordeum spontaneum) and cultivated (Hordeum vulgate ‘Maresi’) barley genotypes under water deficit conditions. Acta Physiol Plant 25:55–61

    Article  CAS  Google Scholar 

  • Bates LS, Waldren RP, Teare JD (1973) Rapid determination of proline for water stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Brueck H, Senbayram M (2009) Low nitrogen supply decreases water-use efficiency of oriental tobacco. J Plant Nutr Soil Sci 172:216–223

    Article  CAS  Google Scholar 

  • Chen QS, Yi KK, Huang G, Wang XB, Liu FY, Wu YR, Wu P (2003) Cloning and expression pattern analysis of nitrogen-starvation-induced genes in rice. Acta Botanica Sinica 45:974–980

    CAS  Google Scholar 

  • Delauney AJ, Vermad PS (1993) Proline biosynthesis and osmoregulation in plants. Plant J 4:215–223

    Article  CAS  Google Scholar 

  • Delauney AJ, Hu CAA, Kishor PBK, Verma DPS (1993) Cloning of ornithine δ-aminotransferase cDNA from Vigna aconitifolia by transcomplementation in Escherichia coli and regulation of proline biosynthesis. J Biol Chem 268:18673–18678

    PubMed  CAS  Google Scholar 

  • Fairless D (2007) Biofuel: the little shrub that could: maybe. Nature 499:652–655

    Article  Google Scholar 

  • Fang CX, He HB, Wang QS, Qiu L, Wang HB, Zhuang YE, Xiong J, Lin WX (2010) Genomic analysis of allelopathic response to low nitrogen and barnyardgrass competition in rice (Oryza sativa L.). Plant Growth Regul 61:277–286

    Article  CAS  Google Scholar 

  • García-Ríos M, Fujita T, Larosa PC, Locy RD II, Clithero JM, Bressan RA, Csonka LN (1997) Cloning of a polycistronic cDNA from tomato encoding γ-glutamyl kinase and γ-glutamyl phosphate reductase. Proc Natl Acad Sci 94:8249–8254

    Article  PubMed  Google Scholar 

  • Gubitz GM, Mittelbach M, Trabi M (1999) Exploitation of the tropical oil seed plant Jatropha curcas L. Bioresour Technol 67:73–82

    Article  CAS  Google Scholar 

  • Hare PD, Cress WA (1997) Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regul 21:79–102

    Article  CAS  Google Scholar 

  • King AJ, He W, Cuevas JA, Freudenberger M, Ramiaramanana D, Graham IA (2009) Potential of Jatropha curcas as a source of renewable oil and animal feed. J Exp Bot 60:2897–2905

    Article  PubMed  CAS  Google Scholar 

  • Kishor PBK, Hong ZL, Miao GH, Hu CAA, Verma DPS (1995) Overexpression of Δ1-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants. Plant Physiol 108:1387–1394

    PubMed  CAS  Google Scholar 

  • Kishor PBK, Sangam S, Amrutha RN, Laxmi PS, Naidu KR, Rao KRSS, Rao S, Reddy KJ, Theriappan P, Sreenivasulu N (2005) Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Curr Sci 88:424–438

    CAS  Google Scholar 

  • Li J, Wu PZ, Li MR, Wu GJ (2007) Development of energy plant: progress and suggestions. Chinese J Nat 29:21–25

    Google Scholar 

  • Schafleitner R, Gaudin A, Rosales ROG, Aliaga CAA, Bonierbale M (2007) Proline accumulation and real time PCR expression analysis of genes encoding enzymes of proline metabolism in relation to drought tolerance in Andean potato. Acta Physiol Plant 29:19–26

    Article  CAS  Google Scholar 

  • Sheehy Skeffington MJ, Jeffrey DW (1985) Growth performance of an inland population of Plantago maritima in response to nitrogen and salinity. Plant Ecol 61:265–272

    Article  Google Scholar 

  • Srinivas V, Balasubramanian D (1995) Proline is a protein-compatible hydrotrope. Langmuir 11:2830–2833

    Article  CAS  Google Scholar 

  • Wu F, Bao W, Li F, Wu N (2008) Effects of drought stress and N supply on the growth, biomass partitioning and water-use efficiency of Sophora davidii seedlings. Environ Exp Bot 63:248–255

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was funded by the National Natural Science Foundation of China (No. 31070301).

Author information

Authors and Affiliations

  1. Key Lab of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, 610064, People’s Republic of China

    Wen-Guo Wang, Rui Li, Bin Liu, Liang Li, Sheng-Hua Wang & Fang Chen

  2. Research Centre of Biomass Energy Technology, Biogas Institute of Ministry of Agriculture, Chengdu, 610041, People’s Republic of China

    Wen-Guo Wang

Authors
  1. Wen-Guo Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rui Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bin Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Liang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sheng-Hua Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Fang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fang Chen.

Additional information

Communicated by G. Klobus.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, WG., Li, R., Liu, B. et al. Effects of low nitrogen and drought stresses on proline synthesis of Jatropha curcas seedling. Acta Physiol Plant 33, 1591–1595 (2011). https://doi.org/10.1007/s11738-010-0692-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-010-0692-6

Keywords

Search

Navigation