Abstract
The influence of exogenous abscisic acid (ABA) on the content of free polysomes (FP), membrane-bound polysomes (MBP), cytoskeleton-bound polysomes (CBP) and cytomatrix-bound polysomes (CMBP) in triticale germs as well as in vitro protein synthesis by these four polysomal fractions were studied. During translation, proteins were biotinylated for chemiluminescence detection. We have found that ABA changed both the content of FP, MBP, CMP and CMBP in germ tissue, and their subsequent translation activity. At 100 μM ABA, the content of FP and MBP was over fourfold lower compared to the control, whereas the amounts of CBP and CMBP were about two- and threefold higher, respectively. Moreover, the estimation of the share of polysomes in each ribosomal fraction (sub-units, monosomes, polysomes) showed that, at 100 μM ABA, cytomatrix-bound polysomes, which constituted 90% of polysomes, were the predominant class in ABA-treated germs while membrane-bound polysomes, which made up 82% of polysomes, dominated in the control. A high level of CMBP in ABA-treated tissues may indicate that this class of polysomes participates in ABA-induced synthesis of proteins. In turn, the inhibition of MBP under ABA-treatment is probably due to the delayed protein synthesis which takes place on these polysomes. We identified two lysine-containing proteins synthesized on both of the above classes of polysomes, whose synthesis was altered due to ABA application. Synthesis of a 47 kDa protein on MBP was inhibited, while synthesis of a 79 kDa protein on CMBP is strongly enhanced by ABA influence. The importance of these findings is discussed.
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Introduction
Along with other regulators of plant growth and development, ABA plays a major role in the control of embryo maturation and seed formation via regulation of storage reserve deposition, acquisition of desiccation tolerance as well as induction of primary dormancy and suppression of precocious germination (Still et al. 1994; Bartels et al. 1988; Gerjets et al. 2010). Although more research dealing with mechanisms of ABA action has been conducted, many questions remain unanswered. It is well known that ABA affects the induction of genes for some specific proteins (Still et al. 1994; Mukherjee et al. 2006; Cao et al. 2007; Xiang et al. 2008) and the inhibition of certain reserve mobilizing enzymes (Robertson et al. 1989; Gómez-Cadenas et al. 1999, 2001; Bethke et al. 2006). Some studies show that ABA also has the ability to modify the orientation of microtubules, a cytoskeletal element directing mRNA transport and localization, which may lead to the alteration of targeted synthesis of proteins within the cell (Shibaoka 1994; Klyachko 2003). Furthermore, ABA may also elicit changes in the process of polysome formation, an event essential for protein synthesis (Weidner et al. 2003). Polysomes consist of two or more ribosomes on an mRNA strand and their formation takes place in different cell compartments. In addition to the two common types: free polysomes (FP) and membrane-bound polysomes (MBP), there is a wealth of evidence suggesting that both cytoskeleton-bound (CBP) and cytoskeleton-membrane bound (cytomatrix-bound) polysomes (CMBP) exist in plant cells (Davies and Abe 1995; Abe et al. 2003). It is proposed that the different types of polysomes are responsible for synthesis of various kinds of proteins. FP are thought to be involved primarily in the synthesis of cytosolic proteins, MBP—secreted, sequestered and membrane proteins, CBP—cytoskeletal, viral or stress proteins (Krupińska et al. 2003). There are also some studies indicating that CMBP is involved in storage protein synthesis (Stanković et al. 1993; Abe et al. 2003).
The aim of the study was to estimate the influence of ABA on FP, MBP, CBP and CMBP formation as well as identification of newly formed proteins, synthesized by these four populations of polysomes. Williamson and Quatrano (1988) suggested that effects of exogenous ABA on embryo-specific gene expression in mature grain embryos, 1–2 days postimbibition, is comparable to the effects of ABA on immature embryos. Therefore, all experiments were conducted after 48 h exposure of triticale caryopses to ABA treatment.
Materials and methods
Plant material and germination conditions
The experiments were conducted on cv. Ugo triticale (Triticosecale) caryopses, supplied by the Plant Cultivation Station in Strzelce. Seeds were surface disinfected in 0.5% sodium hypochlorite for 20 min and washed with sterilized water. Twenty intact grains were placed on two layers of Whatman paper no 1 (Whatman, Maidstone, Kent, the UK) in a Petri dish (∅ 9 cm) containing 15 ml solutions of abscisic acid (10 or 100 μM) and incubated for 48 h in the darkness at 20°C. Grains were scored as germinated when the radicle was ≥1 mm long. In addition, some seeds were also germinated in distilled water for comparison with tested samples. Dry weight was determined by placing 200 mg of fresh weight of embryos/germs in an oven at 105°C for 24 h.
Polysome isolation and quantification
The cytoskeleton-stabilizing buffer C (Abe and Davies 1991), consisting of 5 mM HEPES, 10 mM Mg(OAc)2, 2 mM EGTA, 1 mM PMSF adjusted to pH 7.5 with 9.8 mM KOH, was used for isolation of cytoskeleton fractions from triticale germs after 48 h of caryopses germination. This buffer allows sequential isolation of four polysome populations (Davies and Abe 1995): FP (free polysomes), MBP (membrane-bound polysomes), CBP (cytoskeleton-bound polysomes) and CMBP (cytoskeleton-membrane-bound polysomes). All the fractions were layered on a 0.5 ml “pad” of 50% (w/v) sucrose in buffer B (50 mM Tris–HCl, pH 7.5; 20 mM KOAc, 10 mM Mg(OAc)2 and centrifuged for 1.5 h at 300,000g in a Beckman SW 55 Ti Rotor. The polysomal pellets were rinsed in water and then resuspended in 350 μl of 0.5% PTE. The resuspended polysomes were centrifuged at top speed (approximately 18,000g) for 2 min in a microfuge prior to layering 0.2 ml aliquots (about 15 OD units) on linear 15–60% (w/v) sucrose gradients in buffer B and centrifuged at 300,000g in a Beckman SW 55 Ti Rotor for 45 min. The gradients were subsequently scanned at 254 nm on a UA-5 flow recorder (ISCO, Lincoln, NE, USA) to display subunits, monosomes and polysomes. All the operations were conducted at 0–4°C. More details of the methods applied were described in an earlier paper (Weidner et al. 2003). Levels of polysomes were determined by measuring the area under the polysomal profile after subtracting the gradient baseline OD (absorbance of the gradient loaded with 0.2 ml of resuspension buffer). Quantitation of the ribosomes was done assuming that the absorbance of a 1% solution of ribosomes (measured in a cuvette with a 1 cm optical path at 260 nm) equals 135 (Gualerzi and Cammarano 1969).
In vitro translation with incorporation of biotinylated lysisne
Protein synthesis on different polysomal fractions (FP, MBP, CBP and CMBP) was conducted in nuclease-treated rabbit reticulocyte lysate using biotin-Lys-tRNALys plus the remaining amino acids mix (Promega). Following the isolation of polysomes, 1.5 OD units of each polysomal fraction were added per 50 μl of total reaction mixture. After incubation at 30°C for 90 min, the mixtures were placed on ice to stop the reaction. Additionally, two control reactions were performed containing: (1) no RNA (to exclude any background) and (2) the 1 μg of luciferase RNA (to test synthesis of lysine-rich luciferase). Luciferase control was added to sample reactions in order to estimate the regularity of conducted translation.
Detection of newly synthesized proteins by western-blotting and chemiluminescence
After in vitro translation, 1 μl aliquot from the each 50 μl translation reaction was mixed with 15 μl of SDS sample buffer. After denaturation, biotinylated proteins were separated in 12% SDS-polyacrylamide gel (SDS-PAGE) according to Laemmli (1970), transferred to polyvinylidene difluoride membrane (PVDF, Osmonics), and visualized by binding Streptavidin-HRP, followed by chemiluminescent detection (Transcend™ Chemiluminescent Translation Detection System, Promega). To determine the apparent weight of the translated biotinylated proteins, biotinylated protein standards (Bio-Rad) were used.
Results
Four fractions of polysomes: FP, MBP, CBP and CMBP from triticale germs were isolated after 48 h inhibition of seed germination induced by 10 or 100 μM ABA. Polysomes were successively isolated by using buffer C and its derivates, which stabilized the cytoskeleton. Changes in contents of these populations in gram of tissue dry weight under ABA treatment are shown in Fig. 1. The most abundant fraction in the examined control tissue was composed of free polysomes, whereas the smallest fraction consisted of cytomatrix-bound polysomes. A completely reverse situation was observed under ABA influence. Application of abscisic acid induced a decrease in free (FP) as well as in membrane-bound polysomes (MBP), and an increase in cytoskeleton- (CBP) and cytomatrix-bound polysomes (CMBP). Changes in the content of each polysomal fraction were ABA dose-dependent. It should be emphasized that the predominant polysome population in samples treated with 10 or 100 μM ABA was CMBP, which increased over two- and threefold, respectively, versus the control.
Figure 2 shows the sedimentation profiles of particular polysomal populations in 15–60% sucrose gradients. Percentages of polysomes in each total ribosomal fraction (subunits + monosomes + polysomes) were quantified in order to approximately estimate their translational activity. If the rates of attachment of ribosomes (initiation), movement of ribosomes (translocation) and detachment of ribosomes (termination) are similar for different messages, the number of ribosomes per message will be proportional to its length. The proportion of ribosomes from control tissue existing as polysomes was differential and ranged 62–80% in the four fractions. At 10 and 100 μM ABA, the percentage of FP was greatly reduced, from 80 to 45 and 30%, respectively. Similarly, the share of MBP was also lower, decreasing from 82 to 72 and 58% in an ABA dose-dependent way. In contrast, the share of both CBP and CMBP increased under ABA treatment. CBP and CMBP reached only about 63% in the control, increasing significantly under the influence of 10 and 100 μM ABA to about 80 and 87%, respectively.
We then checked if there were any differences between FP, MBP, CBP and CMBP in protein synthesis under 100 μM ABA treatments. Western-blotting of biotinylated, lysine-containing proteins, synthesized in vitro on four polysomal fractions from the control and ABA-treated tissue is presented in Fig. 3. Two changes in newly synthesized proteins compared to the control were observed. Differences induced by ABA were noticed in protein synthesis on two different polysomal fractions, MBP and CMBP, being quantitative rather than qualitative. Two completely opposite results between protein synthesis on MBP and CMBP under ABA influence were observed. On the one hand, ABA inhibited synthesis of a 47 kDa protein on MBP but on the other hand, strongly induced a 79 kDa protein synthesis on CMBP.
Discussion
In this study, we have investigated which fraction of polysomes was involved in the protein synthesis under the influence of exogenous ABA. It was demonstrated that the application of ABA during triticale germination induced the largest increase in the cytomatrix-bound polysomes content. If we assume that CMBP is involved in the synthesis of storage proteins, it is possible that ABA could induce genes responsible for the synthesis of these proteins on cytomatrix-bound polysomes.
It is well known that ABA plays a role in promoting the synthesis of storage proteins in cereal seeds, including the most common ones such as globulins (for example GLB1 globulin in maize embryos) and lectin wheat-germ agglutinin (WGA) (Raikhel and Quatrano 1986; Rivin and Grudt 1991; Shakirova et al. 2003). Another group of proteins whose synthesis is enhanced by ABA are late embryogenesis abundant (LEA) proteins involved in the development of desiccation tolerance in seeds. This group of hydrophilic polypeptides includes wheat Em protein, which is produced as ABA peaks during the seed development or can be induced in mature embryos and seedlings under ABA treatment (Ried and Walker-Simmons 1990).
In this study, an increase in CMBP was closely associated with the amount of synthesized 79 kDa protein. Although cereal proteins are poor in lysine, detection of biotinylated proteins applied in this work allows identification of polypeptides containing this aminoacid. Parallel to this component of the research, we separated the proteome from triticale embryos of cv. Ugo triticale (data not shown in this paper) germinated in 100 μM ABA for 48 h (the same conditions as in this study). Having compared the results of these two tests, we can expect that it is not a single protein, but a group of proteins which differ in the isoelectric point. The analysis we performed on a MALDI-TOF mass spectrophotometer and the search in the MASCOT database enabled us to identify three lysine-rich LEA proteins. According to these results (Badowiec et al. 2009), we suggest that LEA can be synthesized on cytomatrix-bound polysomes.
On the other hand, we found that ABA inhibits formation of the membrane-bound polysome (MBP) and synthesis of 47 kDa protein. Some authors suggest that MBP is responsible for synthesis of secreted, sequestered and membrane proteins. It is also well documented that among ABA-inducible proteins there is barley α-amylase inhibitor involved in blocking precocious germination by interference with α-amylase activity (Robertson et al. 1989). We noticed that the synthesis of 47 kDa protein in the control sample was the most intensive on the MBP fraction, whereas the application of ABA inhibits synthesis of this protein. Based on the other research we conducted at the same time, we can suggest that the protein obtained is like a lysine-rich ATP synthetase beta subunit (Badowiec et al. 2009). We can suppose that ABA inhibits the enzymatic activity in embryos also by retarding MBP formation. In this study, we found that ABA has a direct inhibitory influence on enzyme synthesis, which is distinguished from the indirect inhibition of α-amylase activity.
Abbreviations
- ABA:
-
Abscisic acid
- CBP:
-
Cytoskeleton-bound polysomes
- CMBP:
-
Cytoskeleton-membrane-bound polysomes (cytomatrix-bound polysomes)
- EGTA:
-
Ethylene glycol-bis (β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid
- FP:
-
Free polysomes
- HEPES:
-
N-2-hydroxyethylpiperazine-N′2′ethanesulfonic acid
- KOAc:
-
Potassium acetate
- Luc:
-
Luciferase
- MBP:
-
Membrane-bound polysomes
- Mg(OAc)2 :
-
Magnesium acetate
- PMSF:
-
Phenylmethylsulfonyl fluoride
- PTE:
-
Polyoxyethylene-10-tridecyl ether
- Tris:
-
Tris-(hydroxymethyl) aminomethane
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Open Access This is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
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Szypulska, E., Weidner, S. Importance of cytomatrix-bound polysomes to synthesis of lysine-containing proteins in triticale germs under ABA treatment. Acta Physiol Plant 33, 1461–1465 (2011). https://doi.org/10.1007/s11738-010-0682-8
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DOI: https://doi.org/10.1007/s11738-010-0682-8