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Physiological and molecular characterization of salt response of Arabidopsis thaliana NOK2 ecotype

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Abstract

Arabidopsis thaliana is a glycophyte capable to tolerate mild salinity. Although salt sensitivity of this species, a variability of this characteristic was revealed between different ecotypes. This study presents the physiological and molecular characteristics of salt response of two ecotypes, NOK2 and Columbia (Col). Seedlings were cultivated in hydroponics in the presence of 0 or 50 mM NaCl during 25 days. Rosette leaf samples were collected after 19, 22, and 25 days for determination of physiological parameters, and after 18 days for study of DNA polymorphism. Salt treatment decreased rosette dry matter, leaf number, leaf hydration, and leaf surface area. All these effects were significantly more visible in Col than in NOK2. Moreover, the NOK2 leaves accumulated less Na+ and more K+ than those of Col. DNA polymorphism between the two ecotypes was analyzed with codominant molecular markers based on PCR amplification, namely, microsatellites, cleaved amplified polymorphism sequence (CAPS), and single nucleotide polymorphism markers (SNP). Among the 35 tested markers, 17 showed a clear polymorphism and were distributed on the five Arabidopsis chromosomes ending with a genetic map construction. These results could play an important role in the future establishment of cartography of candidate gene controlling the K+/Na+ selectivity of ion transport in leaves, a component of plant salt tolerance.

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References

  • Apse MP, Aharon GS, Blumwald E (1999) Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis. Science 285:1256–1258

    Article  CAS  PubMed  Google Scholar 

  • Arabidopsis genome initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796–815

    Article  Google Scholar 

  • Attia H, Arnaud N, Karray N, Lachaâl M (2008) Long-term effects of mild salt stress on growth, ion accumulation and superoxide dismutase expression of Arabidopsis rosette leaves. Physiol Plant 132:293–305

    Article  CAS  PubMed  Google Scholar 

  • Bell CJ, Ecker JR (1994) Assignment of 30 microsatellite loci to the linkage map of Arabidopsis. Genomics 19:137–144

    Article  CAS  PubMed  Google Scholar 

  • Blumwald E, Aharon GS, Apse MP (2000) Sodium transport in plant cells. Biochim Biophys Acta 1465:140–151

    Article  CAS  PubMed  Google Scholar 

  • Carden DE, Walker DJ, Flowers TJ, Miller AJ (2003) Single-cell measurements of the contributions of cytosolic Na1 and K1 to salt tolerance. Plant Physiol 131:676–683

    Article  CAS  PubMed  Google Scholar 

  • Cho RJ, Mindrinos M, Richards DR, Sapolsky RJ, Anderson M, Drenkard E, Dewdney J, Reuber TL, Stammers M, Federspiel N, Theologis A, Yang W-H, Hubbell E, Au M, Chung EY, Lashkari D, Lemieux B, Dean C, Lipshutz RJ, Ausubel FM, Davis RW, Oefner PJ (1999) Genome-wide mapping with biallelic markers in Arabidopsis thaliana. Nat Genet 23:203–207

    Article  CAS  PubMed  Google Scholar 

  • Dellaporta S, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21

    Article  CAS  Google Scholar 

  • Flowers TJ, Hajibagheri MA, Yeo AR (1991) Ion accumulation in the cell walls of rice plants growing under saline condition: evidence for the Oertli hypothesis. Plant Cell Environ 14:319–325

    Article  Google Scholar 

  • Gay AP, Hauck B (1994) Acclimation of Lolium temulentum to enhanced carbon dioxide concentration. J Exp Bot 45:1133–1141

    Article  Google Scholar 

  • Golldack D, Quigley F, Michalowski CB, Kamasani UR, Bohnert HJ (2003) Salinity stress-tolerant and -sensitive rice (Oryza sativa L.) regulate AKT1-type potassium channel transcripts differently. Plant Mol Biol 51:71–81

    Article  CAS  PubMed  Google Scholar 

  • Gorham J, Bridges J, Dubcovsky J, Dvorak J, Hollington AA, Luo MC, Khan JA (1997) Genetic analysis and physiology of a trait of enhanced K+/Na+ discrimination in wheat. New Phytol 137:109–116

    Article  CAS  Google Scholar 

  • Konieczny A, Ausubel F (1993) A procedure for mapping Arabidopsis mutations using co-dominant ecotype specific PCR-based markers. Plant J 4:403–410

    Article  CAS  PubMed  Google Scholar 

  • Labidi N, Lachaâl M, Chibani F, Grignon C, Hajji M (2002) Variability of the response to NaCl of eight ecotypes of Arabidopsis thaliana. J Plant Nutr 25:2627–2638

    Article  CAS  Google Scholar 

  • Niu X, Bressan RA, Hasegawa PM, Pardo JM (1995) Ion homeostasis in NaCl stress environments. Plant Physiol 109:735–742

    CAS  PubMed  Google Scholar 

  • Peng YH, Zhu YF, Mao YQ, Wang SW, Su WA, Tang ZC (2004) Alkali grass resists salt stress through high [K1] and an endodermis barrier to Na+. J Exp Bot 55:939–949

    Article  CAS  PubMed  Google Scholar 

  • Serrano R, Gaxiola R (1994) Microbials model and salt stress tolerance in plants. Crit Rev Plant Sci 13:121–138

    Article  CAS  Google Scholar 

  • Somerville C, Koornneef M (2002) A fortunate choice: the history of Arabidopsis as a model plant. Nat Rev Genet 3:883–889

    Article  CAS  PubMed  Google Scholar 

  • Wu SJ, Ding L, Zhu JK (1996) SOS1, a genetic locus essential for salt tolerance and potassium acquisition. Plant Cell 8:617–627

    Article  CAS  PubMed  Google Scholar 

  • Zhang HX, Blumwald E (2001) Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit. Nat Biotechnol 19:765–768

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Physiologie et Biochimie de la Tolérance au Sel des Plantes, Faculté des Sciences de Tunis, Campus Universitaire, 1060, Tunis, Tunisia

    Rym Kaddour, Sabah M’rah, Najoua Karray-Bouraoui & Mokhtar Lachaâl

  2. Biochimie & Physiologie Moléculaire des Plantes, SupAgro/INRA, 34060, Montpellier Cedex 1, France

    Cécile Lambert & Pierre Berthomieu

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  1. Rym Kaddour
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  2. Sabah M’rah
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  3. Najoua Karray-Bouraoui
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  4. Cécile Lambert
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  5. Pierre Berthomieu
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  6. Mokhtar Lachaâl
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Correspondence to Rym Kaddour.

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Communicated by J. Sadowski.

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Kaddour, R., M’rah, S., Karray-Bouraoui, N. et al. Physiological and molecular characterization of salt response of Arabidopsis thaliana NOK2 ecotype. Acta Physiol Plant 32, 503–510 (2010). https://doi.org/10.1007/s11738-009-0427-8

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  • DOI: https://doi.org/10.1007/s11738-009-0427-8

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