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Seed vigour-related DNA marker in rice shows homology with acetyl CoA carboxylase gene

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Abstract

Germination potential, i.e. vigour manifested at sensu stricto germination, was studied in 22 rice varieties to distinguish between the genetic control for low vigour (lv) at fresh harvest and lv after ageing of seeds. For identifying seed vigour-associated DNA marker(s), correlative studies between (1) physiological parameters viz. germination of (a) unaged seeds under optimum and cold stressed condition, (b) aged seeds under optimal condition and (2) growth-associated biochemical parameters viz. alcohol dehydrogenase (ADH) and esterase (EST) activity during early germination of fresh seeds and antioxidant potential in dry embryos of fresh and aged (1 year) seeds were undertaken. ADH and EST activity as well as antioxidant potential showed positive correlation with speed of embryo emergence and early seedling growth of seeds. The reduction of antioxidant potential in aged seeds also appeared proportional to germination performance. Random amplified polymorphic DNA analysis between the varieties was correlated with the above-mentioned vigour parameters, using multiple regression analysis. This led to the identification of four bands associated with the vigour trait at 99% significance level; of these, the ~ 900-bp DNA fragment (named as HVAC 19), through basic local alignment search tool nucleotides analysis, revealed a sequence homology with acetyl CoA carboxylase (ACCase) gene of wheat at 94% similarity level.

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Abbreviations

ADH:

Alcohol dehydrogenase (EC 1.1.1.1)

ANOVA:

Analysis of variance

AOSA:

Association of official seed analysis

β-ME:

β-Mercaptoethanol

BLASTN:

Basic local alignment search tool nucleotides

DPPH:

2,2′-Diphenyl-1-1-pycrylhydrazyl

DTT:

Dithiothretol

EDTA:

Ethylene diamine tetraacetic acid

EST:

Esterase (EC 3.1.1.1)

EtBr:

Ethidium bromide

hv:

High vigour

ISTA:

International seed testing association

lv:

Low vigour

MAS:

Marker assistance selection

MTT:

3[4,5-Dimethyl-thiazolyl-2]-2,5-diphenyltetrazolium bromide

NA:

Natural ageing

NAD:

Nicotinamide adenine dinucleotide

PMS:

Phenazoniummethosulphate

PVP:

Polyvinylpyrrolidone

QTL:

Quantitative trait loci

ROS:

Reactive oxygen species

TRIS:

Tris(hudroxymethyl) aminomethane

RE:

Restriction enzymes

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Acknowledgments

We thank Chinsurah Research Station, West Bengal, India and Directorate of Rice Research Station, Hyderabad, India, for providing seed material that was grown for seed production at Bose Institute Experimental farm. One of us (Ms. S. Talai) gratefully acknowledges Director, Bose Institute, for allowing the use of facilities at Bose Institute as an honorary fellow and Principal, Hari Mohan Ghose College for providing study leave for conducting part of this research. We thank Mrs Kaberi Ghosh for helping in sending the manuscript online.

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  1. Botany Department, Hari Mahan Ghose College, J 206 & 208A, Paharpur Road, Kolkata, 700 024, India

    Subhra Talai

  2. Botany Department, Bose Institute, 93/1 APC Road, Kolkata, 700 009, West Bengal, India

    Swati Sen-Mandi

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  1. Subhra Talai
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Correspondence to Swati Sen-Mandi.

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Communicated by F. Corbineau.

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Talai, S., Sen-Mandi, S. Seed vigour-related DNA marker in rice shows homology with acetyl CoA carboxylase gene. Acta Physiol Plant 32, 153–167 (2010). https://doi.org/10.1007/s11738-009-0392-2

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