Abstract
Drought stress causes yield loss in rice by inhibiting panicle exsertion which is driven by peduncle elongation near flowering. To elucidate the mechanism of peduncle elongation, we examined the drought sensitivity of corresponding genes encoding xyloglucan endotransglycosylase/hydrolase (XTH), a cell wall-loosening enzyme essential for cell elongation in the peduncle elongation zone at heading stage. When drought was imposed 6 days before heading, peduncle elongation rate (PER) was inhibited significantly at heading and 1 day after heading. Eleven OsXTH genes were isolated and their expression in rice peduncle determined. The expression of 11 OsXTH genes showed different patterns of response to drought stress. OsXTH3 was completely suppressed by drought. Transcript levels for OsXTH19, -20, -24, -27 and -28 genes were markedly down-regulated at a series of drought stresses. By contrast, OsXTH5 was up-regulated. Also, the behavior of leaf gas exchange and peduncle abscisic acid (ABA) level was determined under drought conditions. The results indicated that stomatal conductance was 83% lower than that in control plants and peduncle ABA increased ninefold with drought stress. Peduncle ABA content correlated highly with leaf stomatal conductance and PER (r = 0.85** and 0.88**). The expression in OsXTH5, -19, -20, -24 and -28, corresponded to changes in PER and ABA. We conclude that ABA could be involved in inducing the retardation of PER and changes of expression of OsXTH genes. OsXTH5, -19, -20, -24 and -28 genes may play a role in rice peduncle elongation. In addition, interactions among genes may affect PER under drought.
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References
Boonjung H, Fukai S (1996) Effects of soil water deficit at different growth stages on rice growth and yield under upland conditions. 2. Phenology, biomass production and yield. Field Crops Res 48:47–55
Campbell P, Braam J (1999) Xyloglucan endotransglycosylases: diversity of genes, enzymes and potential wall-modifying functions. Trends Plant Sci 4(9):361–366
Ekanayake IJ, De Datta SK, Steponkus PL (1989) Spikelet sterility and flowering response of rice to water stress at anthesis. Ann Bot 63:257–264
Fricke W (1997) Cell turgor, osmotic pressure and water potential in the upper epidermis of barley leaves in relation to cell location and in response to NaCl and air humidity. J Exp Bot 48:45–58
Fry SC (2004) Tansley review: primary cell wall metabolism: tracking the careers of wall polymers in living plant cells. New Phytol 161:641–675
Fry SC, Smith RC, Renwick KF, Martin DJ, Hodge SK, Matthews KJ (1992) Xyloglucan endotransglycosylase, a new wall-loosening enzyme activity from plants. Biochem J 282:821–828
Fulai Liu, Jensen CR, Andersen MN (2004) Drought stress effect on carbohydrate concentration in soybean leaves and pods during early reproductive development: its implication in altering pod set. Field Crops Res 86:1–13
Ji XM, Raveendran M, Oane R, Ismail A, Lafitte R, Bruskiewich R, Cheng SH, Bennett J (2005) Tissue-specific expression and drought responsiveness of cell-wall invertase genes of rice at flowering. Plant Mol Biol 59:945–964
Liu XZ, Huang BR (2005) Root physiological factors involved in cool-season grass response to high soil temperature. Env Exp Bot 53:233–245
Mackill DJ, Coffman WR, Garrity DP (1996) Varietal improvement for rainfed lowland rice in South and Southeast Asia: results of a survey. In: Rainfed lowland rice improvement, IRRI, Manila, Philippines, pp 242
Mahouachi J, Go’mez-Cadenas A, Primo-Millo E, Talo’n M (2005) Antagonistic changes between abscisic acid and gibberellins in citrus fruits subjected to a series of different water conditions. J Plant Growth Regul 24:179–187
Munns R, Passioura JB, Guo J, Chazen O, Gramer GR (2000) Water relations and leaf expansion: importance of time scale. J Exp Bot 51:1495–1504
Nakamura T, Yokoyama R, Tomita E, Nishitani K (2003) Two azuki bean XTH genes, VaXTH1 and VaXTH2, with similar tissue-specific expression profiles, are differently regulated by auxin. Plant Cell Physiol 44:16–24
Nishitani K, Tominaga R (1992) Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule. J Biol Chem 287(29):21058–21064
O’Toole JC, Namuco OS (1983) Role of panicle exsertion in water stress induced sterility. Crop Sci 23:1093–1097
Okazawa K, Sato Y, Nakagawa T, Asada K, Kato I, Tomita E, Nishitani K (1993) Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls. J Biol Chem 268:25364–25368
Pantuwan G, Fukai S, Cooper M, Rajatasereekul S, O’Toole JC (2002) Yield response of rice (Oryza sativa L.) genotypes to different types of drought under rainfed lowlands. 3. Plant factors contributing to drought resistance. Field Crops Res 73:181–200
Peters WS, Bernstein N (1997) The determination of relative elemental growth rate profiles from segmental growth rates. Plant Physiol 113:1395–1404
Potter I, Fry SC (1993) Xyloglucan endotransglycosylase activity in pea internodes: effects of applied gibberellic acid. Plant Physiol 103:235–241
Rose JKC, Braam J, Fry SC, Nishitani K (2002) The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature. Plant Cell Physiol 43(12):1421–1435
Serpe MD, Matthews MA (1992) Rapid changes in cell wall yielding of elongating Begonia argenteo-guttata L. leaves in response to changes in plant water status. Plant Physiol 100:1852–1857
Setter TL, Flannigan BA, Melkonian J (2001) Loss of kernel set due to water deficit and shade in maize: carbohydrate supplies, abscisic acid, and cytokinins. Crop Sci 41:1530–1540
Uozu S, Tanaka-Ueguchi M, Kitano H, Hattori K, Matsuoka M (2000) Characterization of XET-related genes of rice. Plant Physiol 122:853–860
Weiler W, Wieczorek U (1981) Determination of femtomol quantities of gibberellic acid by radioimmunoassay. Planta 152:159–167
Wu Y, Spollen WG, Sharp RE, Hethering PR, Fry FC (1994) Root growth maintenance at low water potentials: increased activity of xyloglucan endotransglycosylase and its possible regulation by abscisic acid. Plant Physiol 106:607–615
Xu W, Campbell P, Vargheese AK, Braam J (1996) The Arabidopsis XET related gene family: environmental and hormonal regulation of expression. Plant J 9:879–889
Yokoyama R, Nishitani K (2001) A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis. Plant Cell Physiol 42:1025–1033
Yokoyama R, Rose JKC, Nishitani K (2004) A surprising diversity and abundance of xyloglucan endotransglucosylase/hydrolases in rice: classification and expression analysis. Plant Physiol 134:1088–1099
Zhang J, Davies WJ (1990) Does ABA in the xylem control the rate of leaf growth in soil-dried maize and sunflower plants? J Exp Bot 41:1125–1132
Zheng X, Van Huystee RB (1992) Peroxidase-regulated elongation of segments from peanut hypocotyls. Plant Sci 81:47–56
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Communicated by M. Rapacz.
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He, H., Serraj, R. & Yang, Q. Changes in OsXTH gene expression, ABA content, and peduncle elongation in rice subjected to drought at the reproductive stage. Acta Physiol Plant 31, 749–756 (2009). https://doi.org/10.1007/s11738-009-0287-2
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DOI: https://doi.org/10.1007/s11738-009-0287-2