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Impact of hypoxia on the growth and alkaloid accumulation in Catharanthus roseus cell suspension

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Abstract

The Madagascar periwinkle (Catharanthus roseus) produces numerous indole alkaloids, several of which have an important pharmaceutical uses such as ajmalicine, vinblastine and vincristine. The relationship between hypoxia and ajmalicine production in a cell suspension culture of C. roseus were investigated during the cycle of cell culture, in correlation with the effects on growth. The results show that the lack of oxygenation in C20D cells provokes a very strong inhibition in accumulation of the alkaloids and of other possible substances. Moreover, the addition of loganin, a metabolic intermediate of the biosynthetic pathway, in the culture medium of cells subjected to hypoxia restored the alkaloid production. Also, the results showed that the addition of benzyladenine (BA) to the culture medium increased the ajmalicine production and that the inhibitory effect of hypoxia was almost absent in these conditions. Therefore, it could be suggested that BA can without doubt decrease the effects of the hypoxia and increase the ajmalicine production in periwinkle cell suspensions.

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Abbreviations

Log:

Loganin

BA:

Benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

Hyp:

Hypoxia

FM:

Fresh mass

DM:

Dry mass

C:

Control

Ajm:

Ajmalicine

D:

Day

TLC:

Thin layer chromatography

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Authors and Affiliations

  1. University of Larbi Ben M’hidi, Oum El Bouaghi, Algeria

    Mohammed Mourad Senoussi & Bassa Nora

  2. Department of Plant Molecular and Cell Biology, Faculty of Pharmacy, 31 avenue monge, 37200, Tours, France

    Crèche Joêl

Authors
  1. Mohammed Mourad Senoussi
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  2. Bassa Nora
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  3. Crèche Joêl
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Correspondence to Mohammed Mourad Senoussi.

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Communicated by E. Lojkowska.

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Senoussi, M.M., Nora, B. & Joêl, C. Impact of hypoxia on the growth and alkaloid accumulation in Catharanthus roseus cell suspension. Acta Physiol Plant 31, 359–362 (2009). https://doi.org/10.1007/s11738-008-0242-7

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  • DOI: https://doi.org/10.1007/s11738-008-0242-7

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