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Effects of Na2CO3 and NaCl stresses on the antioxidant enzymes of chloroplasts and chlorophyll fluorescence parameters of leaves of Puccinellia tenuiflora (Turcz.) scribn.et Merr.

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Abstract

Antioxidant enzymes in chloroplasts and chlorophyll fluorescence parameters of leaves of Puccinellia tenuiflora (Turcz.) scribn.et Merr. under isotonic Na2CO3 and NaCl stresses were studied. Ascorbate peroxidase (APX) and superoxide dismutase (SOD) activities showed a similar increasing trend and then decreased with the decreasing osmotic potential of culture solution, peaking at −4.74 × 105 Pa under NaCl stress and at −3.40 × 105 Pa under Na2CO3 stress. APX, glutathione transferase and SOD activities were higher under NaCl stress than those under Na2CO3 stress, and higher activities of antioxidant enzymes in chloroplasts were accompanied by lower MDA content under NaCl stress. F v/F m, F v/F o and F v′/F m′ all initially increased and then decreased with the decreasing osmotic potential of culture solution, while Φ PSII, qNP and HDR showed a constant increase. F v/F m, F v/F o, Φ PSII and qNP under NaCl stress were also shown to be higher than those under Na2CO3 stress. The present study suggested that acidity played an important role in the hurt toPuccinellia tenuiflora seedlings, which was due to higher activities of antioxidant enzymes, qNP and Φ PSII, and the Na2CO3 resistance to Puccinellia tenuiflora was also supposed to be less than NaCl resistance in present work.

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Abbreviations

APX:

ascorbate peroxidase

SOD:

superoxide dismutase

GST:

glutathione transferase

ROS:

reactive oxygen species

F m :

maximal fluorescence yield

F m′:

maximal fluorescence yield at actinic light

F o :

initial fluorescence yield

F o′:

minimal fluorescence

F s :

fluorescence yield in steady state of photosynthesis

F v/F m :

maximal photochemical efficiency of PSII

F v/F o :

potential photochemical efficiency

F v′/F m′:

photochemical efficiency of PSII in the light

Φ PSII :

actual photochemical efficiency of PSII in the light

qNP :

non-photochemical quenching coefficient

HDR :

heat dissipation rate

EDTA:

ethylenediaminetetraacetic acid

DTT:

1,4–dithiothreitol

TBA:

thiobarbituric acid

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Acknowledgments

We express our gratitude to our laboratory collaborators for constructive comments and excellent technical assistance. The work in this paper was jointly supported by NSF of China (30770344, 30270234 and 30600073), and 863 Key Sci-tech Special Program (2007AA021402).

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Authors and Affiliations

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou, Jiangsu, 225009, China

    Yuexia Wang & Guorong Sun

  2. College of Biosciences and Biotechnology, Yangzhou University, Yangzhou, 225009, China

    Yuexia Wang, Guorong Sun, Gang Chen, Jianbo Wang & Yan Yan

  3. College of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 201101, China

    Biao Suo

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  1. Yuexia Wang
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  2. Guorong Sun
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  3. Biao Suo
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  4. Gang Chen
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  6. Yan Yan
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Correspondence to Guorong Sun.

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Communicated by G. Bartosz.

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Wang, Y., Sun, G., Suo, B. et al. Effects of Na2CO3 and NaCl stresses on the antioxidant enzymes of chloroplasts and chlorophyll fluorescence parameters of leaves of Puccinellia tenuiflora (Turcz.) scribn.et Merr.. Acta Physiol Plant 30, 143–150 (2008). https://doi.org/10.1007/s11738-007-0102-x

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  • DOI: https://doi.org/10.1007/s11738-007-0102-x

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