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Pretreatment in thidiazuron improves the in vitro shoot induction from leaves in Curculigo orchioides Gaertn., an endangered medicinal plant

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Abstract

Leaf regeneration via direct induction of adventitious shoots obtained from an endangered medicinal plant, Curculigo orchioides Gaertn. by pretreating with thidiazuron. C. orchioides is an endangered medicinal herb belonging to the family Hypoxidaceae. Direct inoculation of leaf pieces on MS medium supplemented with various concentrations of BAP (2–8 μM) or TDZ (2–8 μM) alone or in combination with NAA (0.5 and 1.0 μM) produced low shoot induction both in terms of % response and number of shoots per explant. Hence, leaf explants were pretreated with 15, 25 or 50 μM thidiazuron (TDZ), for 6, 24 or 48 h with the aim of improving shoot regeneration from cultured explants. After pretreatment, explants were transferred to an agar solidified MS medium that was supplemented with BAP (4 μM), TDZ (6 μM), BAP (4 μM) + NAA (1.0 μM), TDZ (6 μM) + NAA (0.5 μM). Control explants were incubated directly on the medium without any pretreatment. The pretreatment of explants with 15 μM TDZ for 24 h significantly promoted the formation of adventitious shoots and the maximum response was observed on MS medium supplemented with 6 μM TDZ. In this medium, 96 % cultures responded with an average number of 16.2 adventitious shoots per explant. The percentage of leaf explants producing shoots and the average number of shoots per explant were significantly improved when TDZ pretreated leaves were cultured onto MS medium supplemented with BAP or TDZ alone or in combination with NAA. The rooted plantlets were successfully transplanted to soil with 90% success. The present investigation indicated the stimulatory role of TDZ pretreatment in regulating shoot regeneration from leaf explants of C. orchioides.

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Abbreviations

BA:

6-Benzylaminopurine

Kn:

Kinetin

MS:

Murashige and Skoog (1962) medium

TDZ:

Thidiazuron (N-phenyl N′ 1,2,3-thidiazol-5-yl urea)

References

  • Anonymous (1979) Jiyangsu college of new medicine, dictionary of Chinese traditional medicine. People’s press, Shanghai, pp 1363

    Google Scholar 

  • Bhamare PB (1998) Traditional knowledge of plants for skin ailments of Dhule and Nandurbar districts, Maharashtra (India). J Phyto Res 11:195–196

    Google Scholar 

  • Bretague B, Chupeau MC, Chupeau Y, Fouilloux G (1994) Improved flax regeneration from hypocotyls using thidiazuron as a cytokinin source. Plant Cell Rep 14:120–124

    Google Scholar 

  • Chandel KPS (1996) Biodiversity in medicinal and aromatic plants in India. In: Ganesh S, Sharma N (eds) Biodiversity in medicinal and aromatic plants in India. ICAR publication, New Delhi, pp 60–61

    Google Scholar 

  • Dhar ML, Dhar MM, Dhawan BN, Mehrotra DN, Ray C (1968) Screening of Indian plants for biological activity. Ind J Exp Biol 6:232–249

    CAS  Google Scholar 

  • D’Onofrio C, Morini S (2006) Somatic embryo, adventitious root and shoot regeneration in in vitro grown quince leaves as influenced by treatments of different length with growth regulators. Sci Hort 107:194–199

    Article  CAS  Google Scholar 

  • Espinosa AC, Pijut PM, Michler CH (2006) Adventitious shoot regeneration and rooting of Prunus serotina in vitro cultures. Hort Sci 41:193–201

    CAS  Google Scholar 

  • Fasolo F, Zimmerman RH, Fordham I (1989) Adventitious shoot formation on excised leaves of in vitro grown shoots of apple cultivar. Plant Cell Tissue Organ Cult 16:75–87

    Article  CAS  Google Scholar 

  • Garg SN, Misra LN, Reddy MN (1989) Corchicoside A, an orcinol glycoside from Curculigo orchioides. Phytochem 28:1771–1772

    Article  CAS  Google Scholar 

  • Geneve RL (2005) Comparative adventitious shoot induction in Kentucky coffee tree root and petiole explants treated with thidiazuron and benzylaminopurine. In Vitro Cell Dev Biol Plant 41:489–493

    Article  CAS  Google Scholar 

  • Gu XF, Zhang JR (2005) An efficient adventitious shoot regeneration system for Zhanhua winter jujube (Zizyphus jujube Mill.) using leaf explants. Plant Cell Rep 23:775–779

    Article  PubMed  CAS  Google Scholar 

  • Guo DP, Zhu ZJ, Hu XX, Zheng SJ (2005) Effect of cytokinins on shoot regeneration from cotyledon and leaf segment of stem mustard (Brassica juncea var. tsatsai) Plant Cell Tissue Organ Cult 83:123–127

    Article  CAS  Google Scholar 

  • Gupta R, Chadha KL (1995) Medicinal and aromatic plants research in India. In: Chadha KL, Gupta R (eds) Advances Horticulture, Medicinal and Aromatic Plants, vol 11. Malhotra House, New Delhi, pp 1–43

  • Hare PD, Van Staden J (1994) Inhibitory effect of thidiazuron on the activity of cytokinin oxidase isolated from soybean callus Plant Cell Physiol 35:1121–1125

    CAS  Google Scholar 

  • Herve´ P, Jauneau A, Pa^ques M, Marien J, Boudet AM, Teulie`res C (2001) A procedure for shoot organogenesis in vitro from leaves and nodes of an elite Eucalyptus gunnii clone: comparative histology. Plant Sci 161:645–653

    Article  CAS  Google Scholar 

  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105–119

    Article  CAS  Google Scholar 

  • Jain SK (1991) Dictionary of Indian folk medicine and ethnobotany. Deep Publications, New Delhi, p 65

    Google Scholar 

  • Kintzios S, Sereti E, Bluchos P, Drossopoulos JB, Kitsaki CK, Liopa-Tsakalidis A (2002) Growth regulator pretreatment improves somatic embryogenesis from leaves of squash (Cucurbita pepo I.) and melon (Cucumis melo I) Plant Cell Rep 21:1–8

    Article  CAS  Google Scholar 

  • Kritikar KR, Basu BD (1935) Indian medicinal plants, vol IV. M/s Bishen Singh Mahendra pal singh, Delhi, pp 2469–2470

  • Kurma SR, Mishra SH (1995) Studies on Curculigo orchioides for antiinflammatory and hepatoprotective activities. Indian Drugs 33:20–25

    Google Scholar 

  • Landi L, Mezzetti B (2006) TDZ, auxin and genotype effects on leaf organogenesis in Fragaria. Plant Cell Rep 25:281–288

    Article  PubMed  CAS  Google Scholar 

  • Lee YK, Chung WI, Ezura H (2003) Efficient plant regeneration via organogenesis in winter squash (Cucurbita maxima Duch) Plant Sci 164:413–418

    Article  CAS  Google Scholar 

  • Lu C (1993) The use of thidiazuron in tissue culture. In Vitro Cell Dev Biol Plant 29:92–96

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol Plant 15:473–477

    Article  CAS  Google Scholar 

  • Murthy BNS, Murch SJ, Saxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Biol Plant 34:267–275

    CAS  Google Scholar 

  • Passey AJ, Barrett KJ, James DJ (2003) Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria × ananassa Duch.) using a range of explant types Plant Cell Rep 21:397–401

    PubMed  CAS  Google Scholar 

  • Prajapati HA, Patel DH, Mehta SR, Subramanian RB (2003) Direct in vitro regeneration of Curculigo orchioides Gaertn., an endangered anticarcinogenic herb Curr Sci 80:747–749

    Google Scholar 

  • Prajapati HA, Mehta SR, Subramanian RB (2004) In vitro regeneration in Curculigo orchioides Gaertn. An endangered medicinal herb. Phytomorp 54:85–95

    Google Scholar 

  • Prathanturarug S, Soonthornchareonnon N, Chuakul W, Phaidee Y, Saralamp P (2003) High-frequency shoot multiplication in Curcuma longa L.using thidiazuron Plant Cell Rep 21:1054–1059

    Article  PubMed  CAS  Google Scholar 

  • Prathanturarug S, Soonthornchareonnon N, Chuakul W, Phaidee Y, Saralamp P (2005) Rapid micropropagation of Curcuma longa using bud explants precultured in thiadiazuron supplemented liquid medium. Plant Cell Tissue Organ Cult 80:347–351

    Article  CAS  Google Scholar 

  • Raghu AV, Geetha SP, Martin G, Balachandran I, Ravindran PN (2006) Direct shoot organogenesis from leaf explants of Embelia ribes Burm. F.: a vulnerable medicinal plant J For Res 11:57–60

    Article  Google Scholar 

  • Shan Z, Raemakers K, Tzitzikas EN, Ma Z, Visser RGF (2005) Development of a highly efficient, repetitive system of organogenesis in soybean (Glycine max (L.) Merr) .Plant Cell Rep 24:507–512

    Article  PubMed  CAS  Google Scholar 

  • Singh SK, Syamal MM (2001) A short preculture in thidiazuron or forchlorfenuron improves axillary shoot proliferation in rose micropropagation. Sci Hort 91:169–177

    Article  CAS  Google Scholar 

  • Suri SS, Arora DK, Sharma R, Ramawat KG (1998) Rapid micropropagation through direct somatic embryogenesis and bulbil formation from leaf explants in Curculigo orchioides Ind J Exp Biol. 36:1130–1135

    Google Scholar 

  • Suri SS, Jain S, Ramawat KG (1999) Plantlet regeneration and bulbil formation in vitro from leaf and stem explants of Curculigo orchioides, an endangered medicinal plant Sci Hort 79:127–134

    Article  CAS  Google Scholar 

  • Tandon M, Shukla YN (1995) Phytoconstituents of Asparagus adscendens, Chlorophytum arundinaceum and Curculigo orchioides: a review Curr Res Med Aromat Plants 17:42–50

    Google Scholar 

  • Thomas TD, Jacob A (2004) Direct somatic embryogenesis of Curculigo orchioides Gaertn., an endangered medicinal herb. J Plant Biotecnnol 6:193–197

    Google Scholar 

  • Van Nieuwkirk K, Zimmerman RH, Fordham I (1987) Thidiazuron stimulation of apple shoot proliferation in vitro. Hort Sci 21:516–518

    Google Scholar 

  • Visser C, Qureshi J, Gill R, Saxena PK (1992) Morphoregulatory role of thidiazuron. Substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl cultures. Plant Physiol 99:1704–1707

    Article  PubMed  CAS  Google Scholar 

  • Xu JP, Xu RS (1992) Cycloartane type sapogenins and their glycoside from Curculigo orchioides. Phytochem 31:2455–2458

    Article  CAS  Google Scholar 

  • Xu JP, Xu RS, Li XY (1992) Four new cycloartane saponins from Curculigo orchioides. Planta Med 58:208–210

    Article  PubMed  CAS  Google Scholar 

  • Yancheva SD, Golubowicz S, Fisher E, YadunSL, Flaishman MA (2003) Auxin type and timing of application determine the activation of the developmental program during in vitro organogenesis in apple. Plant Sci 165:299–309

    Article  CAS  Google Scholar 

  • Zhang O, Chen J, Henny RJ (2005) Direct somatic embryogenesis and plant regeneration from leaf, petiole, and stem explants of Golden Pothos. Plant Cell Rep 23:587–595

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

I acknowledge the financial assistance from Department of Science and Technology (DST), Govt. of India, in the form of a young scientist project (Project No. SR/FTP/LSA-06/2002). I thank the Principal, St. Thomas College, Pala, for providing the necessary laboratory facilities.

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  1. Postgraduate and Research Department of Botany, St. Thomas College, Pala, Arunapuram (P.O), 686 574, Kottayam (Dt.), Kerala, India

    T. Dennis Thomas

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  1. T. Dennis Thomas
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Correspondence to T. Dennis Thomas.

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Communicated by E. Lojkowska.

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Thomas, T.D. Pretreatment in thidiazuron improves the in vitro shoot induction from leaves in Curculigo orchioides Gaertn., an endangered medicinal plant. Acta Physiol Plant 29, 455–461 (2007). https://doi.org/10.1007/s11738-007-0055-0

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  • DOI: https://doi.org/10.1007/s11738-007-0055-0

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