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\Cytokinin oxidase/dehydrogenase (CKX) activity in wild and ethylene-insensitive mutant eti5 type of Arabidopsis thaliana (L.) Heynh plants and the effect of cytokinin N1-(2-chloro-4-pyridyl)-N2-phenylurea on enzymatic activity and leaf morphology

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Abstract

The specific activity of cytokinin oxidase/dehydrogenase (EC 1.5.99.12) (CKX) was determined in leaves of wild type (wt) and ethylene-insensitive mutant (eti5) of Arabidopsis thaliana (L.) Heynh plants. Comparative studies showed that this mutation has lower basal CKX activity than wt. Application of 4PU-30 (N1-(2-chloro-4-pyridyl)-N2-phenylurea) resulted in decreased CKX activity in both wt and mutant plants. The treatment increased leaf blade thickness and the volume of chlorophyll-containing cells per unit leaf area in wt but these changes were not observed in the eti5 mutant. The reduction in chlorophyll “a” and “b”, as well as in carotenoids content in the treated wt tissues resulting from altered leaf morphology was not detected in eti5 plants.

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Abbreviations

4PU-30-N1-(2-chloro-4-pyridyl)-N2-phenylurea CKX:

cytokinin oxidase/dehydrogenase

iP:

iso-pentenyladenine

SE:

Standard error

FW:

fresh weight

dZR:

dihydrozeatin riboside

iPA:

iso-pentenyladenosine

wt :

wild type

ZR:

zeatin riboside

dZ:

dihydrozeatin

Z:

zeatin

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Authors and Affiliations

  1. Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113, Sofia, Bulgaria

    Dessislava Todorova, Irina Vaseva-Gemisheva, Petar Petrov, Ekaterina Stoynova-Bakalova, Vera Alexieva & Emanuil Karanov

  2. Institute of Biological Sciences, University of Wales, Cledwyn Building, Aberystwyth, SY23 3DD, Ceredigion, UK

    Aileen Smith & Michael Hall

Authors
  1. Dessislava Todorova
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  2. Irina Vaseva-Gemisheva
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  3. Petar Petrov
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  4. Ekaterina Stoynova-Bakalova
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  5. Vera Alexieva
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  6. Emanuil Karanov
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  7. Aileen Smith
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  8. Michael Hall
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Correspondence to Dessislava Todorova.

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Todorova, D., Vaseva-Gemisheva, I., Petrov, P. et al. \Cytokinin oxidase/dehydrogenase (CKX) activity in wild and ethylene-insensitive mutant eti5 type of Arabidopsis thaliana (L.) Heynh plants and the effect of cytokinin N1-(2-chloro-4-pyridyl)-N2-phenylurea on enzymatic activity and leaf morphology. Acta Physiol Plant 28, 613–617 (2006). https://doi.org/10.1007/s11738-006-0057-3

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  • DOI: https://doi.org/10.1007/s11738-006-0057-3

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