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Interaction between cadmium, zinc and silver-substituted plastocyanin and cytochrome b 6 f complex — heavy metals toxicity towards photosynthetic apparatus

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Abstract

This paper reports the results of research on the interaction between the cytochrome f of the active cytochrome b 6 f complex (incubated with Cd-, Zn-, and Ag-substituted plastocyanins) and Cu-plastocyanin. The presented studies show, that the metal derivatives of plastocyanin can have an influence on the photosynthetic electron transfer path: cytochrome b 6 f complex — photosystem I. The metal-substituted plastocyanins occupy the plastocyanin electron transfer site of the cytochrome f. The stopped-flow measurements show, that although the metal derivatives of plastocyanin do not influence the rate of cyt f- Pc electron transfer, creation of the non-electron-transfer complexes characterised by a strong binding between the cyt f and substituted plastocyanins and their slow release, dependent on the redox state of the substituted metal, results in the decrease of a turnover of the cytochrome complex.

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Abbreviations

cyt bf :

cytochrome b 6 f complex

cyt b :

cytochrome b

cyt f :

cytochrome f

Pc:

plastocyanin

Ag-, Zn-, Cd-Pc:

silver, zinc and cadmium substituted plastocyanins

PS I:

photosystem I

ISP:

Iron-Sulphur Rieske Protein

EDTA:

ethylenediaminetetraacetic acid

HECAMEG:

(6-O-(N-heptylcarbamoyl)-methyl-α-D-glucopyranoside

HEPES:

N-2 hydroxyethylpiperazine-N’-2-ethanesulfonic acid

FeCy:

potassium ferricyanide [K3Fe(CN)6]

Na-Asc:

sodium ascorbate

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Authors and Affiliations

  1. Department of Physics, University of Agriculture, Akademicka 13, 20-950, Lublin, Poland

    Agnieszka Sujak

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  1. Agnieszka Sujak
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The research was done in the Department of Plant Biochemistry, Freiburg University, Schänzlestrasse 1, 79 104 Freiburg, Germany

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Sujak, A. Interaction between cadmium, zinc and silver-substituted plastocyanin and cytochrome b 6 f complex — heavy metals toxicity towards photosynthetic apparatus. Acta Physiol Plant 27, 61–69 (2005). https://doi.org/10.1007/s11738-005-0037-z

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  • DOI: https://doi.org/10.1007/s11738-005-0037-z

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