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Desiccation-induced changes in lipid peroxidation, superoxide level and antioxidant enzymes activity in neem (Azadirachta indica A. Juss) seeds

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Abstract

The freshly harvested mature neem seeds (42.2 % seed moisture content) with 100 % viability deteriorate when naturally desiccated to below 10.9 %. The desiccation-induced loss of viability was closely associated with over accumulation of superoxide anion and lipid peroxidation products both in the embryonic axes and cotyledons. The levels of superoxide anion and lipid peroxidation products were higher in axes compared to cotyledons. Superoxide dismutase activity was not much affected, both in the axes and cotyledons of 100 % viable seeds during desiccation from 42.2 % to 10.9 % seed moisture content. Steep rise in its activity was observed during drying below lowest safe moisture content (LSMC). Activities of catalase and peroxidase exhibited substantially higher levels in the 100 % viable seeds dehydrated up to LSMC. Their activities declined sharply in seeds with water content below LSMC. Impairment of catalase and peroxidase activities possibly lead to enhanced accumulation of reactive oxygen species. The accumulation of superoxide anion, lipid peroxidation and differential expression of superoxide dismutase and catalse/peroxidase activities in response to desiccation (below LSMC) is discussed to explain the intermediate storage physiology of neem seeds.

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Abbreviations

LSMC:

least safe moisture content

MDA:

malondialdehyde

ROS:

reactive oxygen species

SOD:

superoxide dismutase

POD:

peroxidase

CAT:

catalase

dah:

days after harvest

TBARS:

2-thiobarbituric acid reactive substances

O −2 :

superoxide anion

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  1. Seed Biology Laboratory, School of Life Sciences, Pt. Ravishankar Shukla University, 492 010, Raipur, India

    Boby Varghese & S. C. Naithani

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  1. Boby Varghese
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  2. S. C. Naithani
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Varghese, B., Naithani, S.C. Desiccation-induced changes in lipid peroxidation, superoxide level and antioxidant enzymes activity in neem (Azadirachta indica A. Juss) seeds. Acta Physiol Plant 24, 79–87 (2002). https://doi.org/10.1007/s11738-002-0025-5

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  • DOI: https://doi.org/10.1007/s11738-002-0025-5

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