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Early changes in mRNA populations in leaves of ultraviolet-B-treated European beech (Fagus sylvatica L.) seedlings

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Abstract

Changes in the patterns of gene expression were analyzed in ultraviolet-B (UV-B; 280–320 nm) -treated seedlings of European beech by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) of mRNA. Utilizing 12 different downstream primers and 27 degenerated 10-mer upstream primers 50 induced and 29 repressed cDNA bands were detected. Nineteen bands were subcloned and sequenced. Two clones revealed significant homologies to known database sequences: putative nascent polypeptide-associated complex alpha and 60S ribosomal protein L37. However, only the latter was confirmed to be UV-B-induced by northern blot analysis.

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Abbreviations

CHS:

chalcone synthase

DDRT-PCR:

differential display reverse transcriptase polymerase chain reaction

EST:

expressed sequence tag

PAL:

phenylalanine ammonia-lyase

PPFR:

photosynthetic photon fluence rate

UV-B:

ultraviolet B, 280–320 nm

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Authors and Affiliations

  1. GSF - National Research Center for Environment and Health, Institute of Biochemical Plant Pathology, Exposure Chamber Group, D-85764, Neuherberg, Germany

    Dieter Ernst, Ingrid Liegl, Evi Kiefer, Werner Heller & Heinrich Sandermann

  2. GSF - National Research Center for Environment and Health, Institute of Soil Ecology, Exposure Chamber Group, D-85764, Neuherberg, Germany

    Harald K. Seidlitz

Authors
  1. Dieter Ernst
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  2. Ingrid Liegl
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  3. Evi Kiefer
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  4. Harald K. Seidlitz
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  5. Werner Heller
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  6. Heinrich Sandermann
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Correspondence to Dieter Ernst.

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Ernst, D., Liegl, I., Kiefer, E. et al. Early changes in mRNA populations in leaves of ultraviolet-B-treated European beech (Fagus sylvatica L.) seedlings. Acta Physiol Plant 23, 343–349 (2001). https://doi.org/10.1007/s11738-001-0042-9

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  • DOI: https://doi.org/10.1007/s11738-001-0042-9

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