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The effect of aluminium on polypeptide pattern of cell wall proteins isolated from the roots of Al-sensitive and Al-resistant barley cultivars

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Abstract

Accumulation of some proteins isolated from the cell wall of roots of the Al-sensitive (Alfor) and the Al-resistant (Bavaria) barley cultivars were followed during treatment with different Al3+ concentrations, pH changes of the root medium, and several heavy metals (Cu2+, Cd2+, Co2+). SDS-PAGE analysis revealed an Al-induced accumulation of polypeptides with molecular mass of 14, and 16 kDa and a group of polypeptides around 27 kDa. The accumulation pattern of Al-induced polypeptides was very similar in both cultivars but in the Al-resistant Bavaria it was induced at lower Al concentration and earlier than it was in the Al-sensitive cultivar Alfor. Changes in pH values of root medium (pH 3.5–6.5) did not show any effect on the accumulation of Al-induced cell wall polypeptides either in Al-sensitive or in Al-tolerant barley cultivar. Heavy metals (Cu, Cd, and Co) at concentration of 10 µM resulted in similar accumulation of individual polypeptides as we found after Al treatment. In comparison to Al, quantitative differences in polypeptides accumulation induced by Cu, Cd and Co were less expressed that of Al treatment. More pronounced accumulation and earlier induction of individual cell wall polypeptides in roots of Al-resistant barley cultivar than in Al-sensitive, might indicate some possible role of these polypeptides in plant resistance to Al stress.

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Abbreviations

BSA:

bovine serum albumine

DTT:

dithiothreitol

EGTA:

ethyleneglycolbis-(-amino-ethyl ether) N, N′-tetraacetic acid

PVP:

polyvinylpyrrolidone

SHAM:

salicylhydroxamic acid

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Authors and Affiliations

  1. Institute of Botany, Slovak Academy of Sciences, Dúbravská cesta 14, SK-84223, Bratislava, Slovakia

    Ladislav Tamás, Jana Huttová, Lenka Hajasová & Igor Mistrík

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  1. Ladislav Tamás
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  2. Jana Huttová
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  3. Lenka Hajasová
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  4. Igor Mistrík
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Tamás, L., Huttová, J., Hajasová, L. et al. The effect of aluminium on polypeptide pattern of cell wall proteins isolated from the roots of Al-sensitive and Al-resistant barley cultivars. Acta Physiol Plant 23, 161–168 (2001). https://doi.org/10.1007/s11738-001-0004-2

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  • DOI: https://doi.org/10.1007/s11738-001-0004-2

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