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Application of a rapid glucose-6-phosphate isomerase assay in white cabbage breeding

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Abstract

In the studied conditions of cellulose acetate electrophoresis only glucose-6-phosphate isomerase (PGI, EC 5.3.1.9) appeared to be a polymorphic isozyme. The PGI analysis of 23 cabbage (Brassica oleracea var. capitata) inbred lines, carried out in two years, allowed detecting off-type individuals in four lines. Seed contamination caused by sib-pollination was detected in 10 out of 19 F1 hybrids. In five of them the contamination did not exceed 5 %, and in the remaining five, ranged from 27–52 %. Also homozygosity of 67 plants obtained through anther culture in vitro was confirmed. The results indicate that PGI electrophoresis in cellulose acetate matrix is very fast and can be useful in the assessment of genetic purity in cabbage breeding materials.

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Authors and Affiliations

  1. Department of Genetics, Plant Breeding and Seed Science, Faculty of Horticulture, Agricultural University of Kraków, Al. 29 Listopada 54, 31-425, Kraków, Poland

    Rafał Barański

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  1. Rafał Barański
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Barański, R. Application of a rapid glucose-6-phosphate isomerase assay in white cabbage breeding. Acta Physiol Plant 22, 45–51 (2000). https://doi.org/10.1007/s11738-000-0007-4

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  • DOI: https://doi.org/10.1007/s11738-000-0007-4

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