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Regulating effects of different moxibustion therapies on bax and Bcl-2 in rats with ulcerative colitis

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摘要

目的

探讨不同灸法对溃疡性结肠炎(ulcerative colitis, UC)大鼠结肠损伤的改善作用, 以及对Bcl-2 mRNA、 Bax mRNA表达的影响。

方法

采用免疫学方法加局部刺激制备实验性UC大鼠模型。造模后采用不同灸法治疗14 次, 实时荧光定量PCR技术检测结肠组织Bcl-2 mRNA、 Bax mRNA表达。

结果

(1)各治疗组结肠大体形态损伤积分和光镜下组织学损伤积分较模型组比较有明显改善(P<0.05, P<0.01); (2)UC模型大鼠结肠组织Bcl-2 mRNA的表达显著高于正常大鼠(P <0.01), 而Bax mRNA的表达显著低于正常大鼠(P<0.01); (3)治疗后, 隔药灸组、 隔姜灸组、 隔蒜灸组大鼠结肠组织Bcl-2 mRNA的表达明显下调, 与模型组比较有显著差异(P<0.05 或 P<0.01);隔药灸组、 隔姜灸组Bax mRNA的表达明显增高, 与模型组比较有显著差异(P<0.05)。

结论

初步研究结果提示隔药灸、 隔姜灸、 隔蒜灸、 温和灸治疗均能有效改善UC大鼠结肠病理损伤, 隔药灸、 隔姜灸、 隔蒜灸可不同程度抑制UC大鼠结肠组织Bcl-2 mRNA和Bax mRNA的表达, 调节Bcl-2/Bax mRNA的比例关系。

Abstract

Objective

To investigate the improving effects of different moxibustion therapies on colonic injury in rats with ulcerative colitis (UC), and on the expression of Bcl-2 and Bax mRNA.

Methods

Rats model of UC was made with immune methods and local stimulation. The models were treated with different moxibustion therapies 14 times, and then the expression of Bcl-2 and Bax mRNA were tested in the colonic tissue with fluorescent quantitative PCR.

Results

(1)General scores of colon and histologic scores of injury were improved in all treatment groups except the model group (P<0.05, P<0.01); (2) The expression of Bcl-2 mRNA in the model rats of UC was higher than that in the normal rats (P<0.01), whereas Bax lower (P<0.01); (3) After treatments, the expression of Bcl-2 mRNA was lower in the herb cake-partitioned moxibustion, ginger-partitioned moxibustion, and garlic-partitioned moxibustion groups than that in the model group (P<0.05, P<0.01), and Bax mRNA in the herb cake-partitioned and ginger-partitioned groups was higher (P<0.05).

Conclusion

Herb cake-partitioned moxibustion, ginger-partitioned moxibustion, garlic-partitioned moxibustion, and mild moxibustion could improve the pathological injury in UC rats. Herb cake-partitioned moxibustion, ginger-partitioned moxibustion, and garlic-partitioned moxibustion could inhibit the expression of Bcl-2 mRNA and Bax mRNA in the colonic tissue of UC rats to regulate the ratio of Bcl-2/Bax mRNA.

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Authors and Affiliations

  1. Shanghai Research Institute of Acupuncture and Meridian, Shanghai, 200030, P. R. China

    Hui-rong Liu  (刘慧荣), Shuang Zhou  (周爽), Xiao-mei Wang  (王晓梅) & Huan-gan Wu  (吴焕淦)

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  1. Hui-rong Liu  (刘慧荣)
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  2. Shuang Zhou  (周爽)
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  3. Xiao-mei Wang  (王晓梅)
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  4. Huan-gan Wu  (吴焕淦)
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Additional information

Fund Item: Shanghai Leading Academic Discipline Project (S30304)

Author: LIU Hui-rong (1976–), female, associate researcher

Correspondence Author: ZHOU Shuang (1972–), female, associate researcher

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Liu, Hr., Zhou, S., Wang, Xm. et al. Regulating effects of different moxibustion therapies on bax and Bcl-2 in rats with ulcerative colitis. J. Acupunct. Tuina. Sci. 8, 174–180 (2010). https://doi.org/10.1007/s11726-010-0400-5

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  • DOI: https://doi.org/10.1007/s11726-010-0400-5

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