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Dynamic changes of glycine and taurine in corpus striatum of rats with ischemia-reperfusion injury and effects of electroacupuncture

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摘要

目的

研究脑缺血再灌注大鼠纹状体内甘氨酸(Gly)和牛磺酸(Tau)的动态变化及电针的调节作用。

方法

将30 只雄性SD大鼠随机分为假手术组、 模型组、 治疗组。 假手术组制备假的脑缺血再灌注模型。 模型组和治疗组制成脑缺血再灌注模型, 缺血1.5 h, 再灌注3.75 h。 治疗组在模型组基础上电针“风池”穴。 分别观察各组大鼠的神经功能缺损评分; 通过微透析及高效液相技术测定各组大鼠纹状体内Gly及Tau的浓度变化。

结果

脑缺血1.5 h时Gly和Tau浓度显著升高。 再灌注后Gly和Tau浓度迅速降至假手术组水平。 再灌注2∼2.25 h模型组和治疗组Gly浓度再次出现高峰, 随后其浓度逐渐降至假手术组水平。 治疗组Gly浓度于再灌注后2.75 h出现第3 次高峰。 电针可减缓再灌注后Tau浓度下降, 并于再灌注后1.5 h、 2 h、 2.75 h出现3 次高峰, 以最后一次的浓度最高。 模型组和治疗组的神经功能缺损评分在术后显著增高, 术后5.25 h的评分显著减少, 治疗组优于模型组 (P<0.05)。

结论

脑缺血再灌注使纹状体内Gly和Tau浓度在特定时段升高。 电针使Gly和Tau浓度于再灌注后特定时段升高, 这可能是电针脑保护作用的重要机制之一。

Abstract

Objective

To investigate the dynamic changes of glycine (Gly) and taurine (Tau) in corpus striatum of rats with ischemia-reperfusion injury (IRI), and the regulation effect of electroacupuncture on them.

Methods

Thirty SD rats were randomly divided into sham surgery group, model group, and treatment group. Rats in the sham surgery group were operated without ischemia. Rats in the model group and treatment group were operated to make model of cerebral IRI. The ischemia status lasts 1.5 h, and reperfusion was performed for 3.75 h. Rats in the treatment group were treated with electroacupuncture at Fengchi (GB 20). Neurologic deficit score (NDS) was used to evaluate the rats functions. Microdialysis and High Performance Liquid Chromatography technique were used to detect the changes of Gly and Tau in corpus striatum of rats.

Results

Contents of Gly and Tau increased at 1.5 h after ischemia. Them decreased to level as same as those rats in the sham surgery group after reperfusion. The content of Gly in the model and treatment group increased again at 2–2.5 h after reperfusion, and then decreased to the same level of the sham surgery group. There was a third increase of Gly content in the treatment group at 2.75 h after reperfusion. Electroacupuncture treatment could delay the decrease of Tau content after reperfusion, and make it increase at 1.5, 2 and 2.75 h after reperfusion. The peak appeared in the last time. NDS in the model and treatment group were more than that in the sham surgery group, and lowered at 5.25 h after surgery. Effects in treatment group was better than that in the model group (P<0.05).

Conclusion

IRI makes contents of Gly and Tau in corpus striatum increase at a special time. Electroacupuncture treatment could increase contents of Gly and Tau at a special time after reperfusion, which maybe an important mechanism of protecting effects of electroacupuncture on the brain.

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Authors and Affiliations

  1. Shanghai Research Institute of Acupuncture and Meridian, Shanghai, 200030, P. R. China

    Ming-shu Xu  (徐鸣曙), Xin-hui Wang  (王新辉), Zhi-qiang Wang  (王志强) & Yi-jun Cui  (崔毅军)

  2. Shanghai Research Center for Acupuncture and Meridian, Shanghai, 201203, P. R. China

    Lin-Bao Ge  (葛林宝)

  3. Shanghai Research Institute of Qigong, Shanghai, 200030, P. R. China

    Lin-Bao Ge  (葛林宝)

  4. Yueyang Hospital of Integrated Chinese and Western Medicine affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200047, P. R. China

    Jia Xu  (徐佳)

Authors
  1. Ming-shu Xu  (徐鸣曙)
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  2. Lin-Bao Ge  (葛林宝)
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  3. Xin-hui Wang  (王新辉)
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  4. Jia Xu  (徐佳)
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  5. Zhi-qiang Wang  (王志强)
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  6. Yi-jun Cui  (崔毅军)
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Additional information

Fund Items: National Basic Research Program of China (973 Program, 2005CB523306); the Scientific and Technologic Fund of Shanghai (014319364); Shanghai Leading Academic Discipline Project (S30304); Shanghai Municipal Education Commission (06CZ044)

Author: XU Ming-shu (1978–), male, doctor of medicine, attending physician

GE Lin-bao (1951–), male, research fellow

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Xu, Ms., Ge, LB., Wang, Xh. et al. Dynamic changes of glycine and taurine in corpus striatum of rats with ischemia-reperfusion injury and effects of electroacupuncture. J. Acupunct. Tuina. Sci. 7, 129–133 (2009). https://doi.org/10.1007/s11726-009-0129-1

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  • DOI: https://doi.org/10.1007/s11726-009-0129-1

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