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Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up derivative

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Abstract

Antifungal activity of recombinant medicinal leech destabilase-lysozyme (rec.Dest-Lys) was investigated by using fungi: Botrytis cinerea, and Verticillium lateriticum, including yeasts Candida guillermoudii and Shizosaccharomyces pombe. Its antibacterial activity was investigated on gram-negative bacteria Pseudomonas fluorescens. These activities were assessed by radial agar diffusion assay, and scanning and transmission electron microscopy. Therefore, destabilase-lysozyme not only is endo-isopeptidase and lysozyme, but also has antifungal and antibacterial activities. Muramidase activity of rec. Dest-Lys disappeared after heat-treating at 90°C for 50 min without the loss of its antimicrobial activity. Furthermore we showed for the first time that the heated-up derivative of rec.Dest-Lys exhibited more potent activities against the above enumerated fungi and gram-negative bacteria than original protein.

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Authors and Affiliations

  1. Biological Faculty, M. V. Lomonosov Moscow State University, Moscow, 119991, Russia

    T. G. Yudina, Danyang Guo, N. F. Piskunkova, I. B. Pavlova & I. P. Baskova

  2. Institute of Bioorganic Chemistry (RAS), Miklukho-Maklaya 16/10, Moscow, 117992, Russia

    L. L. Zavalova

Authors
  1. T. G. Yudina
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  2. Danyang Guo
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  3. N. F. Piskunkova
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  4. I. B. Pavlova
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  5. L. L. Zavalova
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  6. I. P. Baskova
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Correspondence to Danyang Guo.

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Yudina, T.G., Guo, D., Piskunkova, N.F. et al. Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up derivative. Front. Chem. Sci. Eng. 6, 203–209 (2012). https://doi.org/10.1007/s11705-012-1277-2

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  • DOI: https://doi.org/10.1007/s11705-012-1277-2

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