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Application of high-speed counter-current chromatography coupled with high performance liquid chromatography for the separation and purification of Quercetin-3-O-sambubioside from the leaves of Nelumbo nucifera

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Abstract

Quercetin-3-O-sambubioside [Quercetin-3-O-β-D-xylopyranosyl (1→2)-β-D-glucopyranoside] was separated and purified by semi-preparative high-speed counter-current chromatography (HSCCC) with a two-phase-solvent system composed of ethyl acetate-n-butanol-water (4:1:5, v/v) from the leaves of Nelumbo nucifera (Lotus). A total of 5.0 mg of the targeted compound with a purity of 98.6% as determined by high performance liquid chromatography (HPLC) was obtained from 100 mg of the crude extract cleaned up by AB-8 macroporous resin in a one-step separation. Quercetin-3-O-sambubioside was a novel flavonoid glycoside from the leaves of Nelumbo nucifera, and its chemical structure was identified by means of ESI-MS, 1D NMR and 2D NMR.

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Authors and Affiliations

  1. State Key Lab of Food Science and Technology, Institute for Advanced Study, Nanchang University, Nanchang, 330047, China

    Shengguo Deng, Zeyuan Deng, Yawei Fan, Jing Li, Rong Liu & Dongmei Xiong

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  1. Shengguo Deng
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  2. Zeyuan Deng
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  3. Yawei Fan
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  4. Jing Li
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  5. Rong Liu
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  6. Dongmei Xiong
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Correspondence to Zeyuan Deng.

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Deng, S., Deng, Z., Fan, Y. et al. Application of high-speed counter-current chromatography coupled with high performance liquid chromatography for the separation and purification of Quercetin-3-O-sambubioside from the leaves of Nelumbo nucifera . Front. Chem. Eng. China 3, 375–382 (2009). https://doi.org/10.1007/s11705-009-0247-9

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  • DOI: https://doi.org/10.1007/s11705-009-0247-9

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