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The Effects of Chronically Increased Intra-abdominal Pressure on the Rabbit Diaphragm

  • Animal Research
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Abstract

Background

Diaphragmatic muscular remodeling is caused by various conditions and was mainly studied in pulmonary pathologies and chronic alterations of intra-thoracic pressure. We investigate the effect of the chronically increased intra-abdominal pressure (IAP) on the diaphragm by morphological and biochemical analysis.

Methods

Thirty rabbits were divided into control and study groups. IAP was increased in group B to 12 mmHg for 2 months. The left hemidiaphragm underwent morphological, while the right underwent biochemical analysis.

Results

In H&E, all fibers were normal. ATPase analysis demonstrated that type I fibers show no differences between groups. Type ΙΙΑ were decreased (p = 0.016) while type ΙΙΒ/X fibers were increased (p = 0.025) in group B. Fibers with resistance to fatigue were decreased in group B (p = 0.024). In group B, biochemical activity for glutathione reductase (p = 0.004), glutathione peroxidase (p = 0.021), protein carbonylation (0.029), lipid peroxidation (p = 0.005), and balance of preoxidative–antioxidative factors (p = 0.006) was increased.

Conclusions

Chronically increased IAP induces alterations to the rabbit diaphragm. Adaptation, equivalent to strenuous contraction, transforms the diaphragm to be functionally more efficient toward workload but makes it vulnerable against oxidative stress.

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Acknowledgments

We would like to thank John Martos for taking care of the laboratory animals, Εkaterini Theologou for the technical support concerning the morphologic analysis, and Christina Befani for the assistance concerning the biochemical analysis.

Conflict of Interest

All authors have no conflict of interest to declare.

Author information

Authors and Affiliations

  1. 3rd Department of Surgery, AHEPA University Hospital, Aristotle University of Thessaloniki, Aigaiou 6, 54655, Thessaloniki, Greece

    Theodossis S Papavramidis, Efstathios Kotidis, Konstantinos Ioannidis & Spiros T Papavramidis

  2. Department of Pathology, AHEPA University Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Angeliki Cheva & George Karkavelas

  3. Veterinary School, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Thomai Lazou

  4. Department of Biochemistry, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece

    George Koliakos

Authors
  1. Theodossis S Papavramidis
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  2. Efstathios Kotidis
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  3. Konstantinos Ioannidis
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  4. Angeliki Cheva
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  5. Thomai Lazou
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  6. George Koliakos
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  7. George Karkavelas
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  8. Spiros T Papavramidis
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Correspondence to Theodossis S Papavramidis.

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Papavramidis, T.S., Kotidis, E., Ioannidis, K. et al. The Effects of Chronically Increased Intra-abdominal Pressure on the Rabbit Diaphragm. OBES SURG 22, 487–492 (2012). https://doi.org/10.1007/s11695-012-0587-2

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  • DOI: https://doi.org/10.1007/s11695-012-0587-2

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