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Recent advances and versatility of MAGE towards industrial applications

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Systems and Synthetic Biology

Abstract

The genome engineering toolkit has expanded significantly in recent years, allowing us to study the functions of genes in cellular networks and assist in over-production of proteins, drugs, chemicals and biofuels. Multiplex automated genome engineering (MAGE) has been recently developed and gained more scientific interest towards strain engineering. MAGE is a simple, rapid and efficient tool for manipulating genes simultaneously in multiple loci, assigning genetic codes and integrating non-natural amino acids. MAGE can be further expanded towards the engineering of fast, robust and over-producing strains for chemicals, drugs and biofuels at industrial scales.

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Abbreviations

aaRS:

Aminoacyl–tRNA synthetase

CAGE:

Conjugative assembly genome engineering

CoS-MAGE:

Coselection MAGE

DXP:

1-Deoxy-d-xylulose-5-phosphate

GRO:

Genomically recoded organism

MAGE:

Multiplex automated genomic engineering

MMR:

Mismatch repair

NSAAs:

Non-standard amino acids

ORF:

Open reading frame

RBS:

Ribosome binding site

RF1:

Release factor 1

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Acknowledgments

The authors thank D.K. Chaudhary and S. Prakash for their fruitful discussion and suggestion for the improvement of the manuscript. The authors also appreciate anonymous reviewers of the journal for their valuable comments and suggestions to improve the quality of the manuscript.

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  1. Institute of Systems and Synthetic Biology, Université d’Évry Val d’Essonne, Genopole Campus 1, Batiment Genavenir 6, 5 rue Henri Desbruères, 91030, Évry, France

    Vijai Singh & Darren Braddick

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  1. Vijai Singh
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  2. Darren Braddick
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Correspondence to Vijai Singh.

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Singh, V., Braddick, D. Recent advances and versatility of MAGE towards industrial applications. Syst Synth Biol 9 (Suppl 1), 1–9 (2015). https://doi.org/10.1007/s11693-015-9184-8

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