Abstract
The bacteria, Serratia marcescens (SM1) was previously obtained from the black-winged termite, Odontotermes formosanus Shiraki. SM1 was highly toxic to O. formosanus, however, the mechanism of toxicity is unclear. In this study, toxicity test results showed that the main components that affected O. formosanus were in a supernatant and that the insecticidal protease in the supernatant resulted in the death of O. formosanus. In addition, zinc sulphate recovery experiments indicated that the metalloproteinases in the supernatant were more harmful. These results provide a theoretical foundation for the future biological control of termites, the basis for the development of pest control technology and the discovery of new pesticides.
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Introduction
Termites damage buildings reservoir dams and other structures made of wood, agricultural and forestry crops, and cable transportation facilities(Cosme et al. 2020; Dahlsjo et al. 2020; Djuideu et al. 2020; Du et al. 2020), and hazardous areas account for approximately 50% of the global area. Termites live in social groups and their resistance to natural enemies and adverse environmental factors is greatly enhanced due to their group defense function. In addition, the secluded nature of termites makes them more difficult to study and control. Therefore, the prevention and treatment of termite infestations has always been a challenge of pest management (Zhang et al. 2015). At present, the most common and important way to control termites is to use chemical agents (Sapkota et al. 2020). The advantage of this approach is that it is effective, but chemical pesticides pose considerable threat to the environment and to human health, including causing pesticide poisoning, cancer, deformities in children and gene mutation (Liu et al. 2010). With increasing awareness of the need for safety and environmental protection, these chemical termite control agents will eventually be eliminated. Compared with chemical controls, biological control is environmentally safe and durable, and avoids the problems caused by chemicals. It is foreseen that biological control methods will become the mainstream of future control work.
Serratia marcescens is a rod-shaped, anaerobic, gram-negative bacterium of the family Enterobacteriaceae. It is generally smaller than other intestinal bacteria, without capsules and occasionally with long filaments (Hejazi and Falkner 1997). It is commonly found in water, plants, animals and soil, and produces a secondary metabolite—prodigiosin—in the process of growth (Montaner and Perez-Tomas 2003). S. marcescens is pathogenic to many insects, including the cotton bollworm, Helicoverpa armigera Hüber (Chen et al. 2005), Myrmeleotettix palpalis (Jin et al. 2005), Phyllotreta striolata (Yang et al. 2014), the tobacco cutworm, Spodoptera litura Fabricius (Niu et al. 2015; Aggarwal et al. 2017), S. exigua (Niu et al. 2015), and the larvae and eggs of the Asian palm weevil, Rhynchophorus ferrugineus Oliver (Zhang et al. 2011). Secretory product of S. marcescens, prodigiosin, have also been found to be pathogenic to some organisms, such as Bursaphelenchus xylophilus (Hu et al. 2017), the yellow fever mosquito, Aedes aegypti L. and the Indian malaria mosquito, Anopheles stephensi Liston (Patil et al. 2011). Niu et al. (2018) used S. marcescens mixed with five insecticides to treat Laodelphax striatellus and found that the mortality rate of L. striatellus to the insecticides could be improved. It is clear that S. marcescens is pathogenic to many types of pests and has wide market prospects in biological control.
Our laboratory recently isolated a red pigment-producing bacterium from dead termites and identified it as Serratia marcescens (SM1) (Fu et al. 2019). SM1 was significantly toxic to O. formosanus (Fu et al. 2020), but the specific components and mechanisms of this toxicity need further examination. Research has shown that the pathogenesis of S. marcescens is due to chitinase enzymes (Regev et al. 1996; Zhang et al. 2000; Xu and Peng 2004; Yin et al. 2004; Jin et al. 2005), and Tao 2006 has reported that the insecticidal protein of S. marcescens is a metallic protein that exists in a supernatant of the bacteria. However, the active components of S. marcescens that are toxic to termites have not been identified. The purposes of this study are: (1) to clarify the termite-killing components of S. marcescens; and, (2) to provide a basis for the biological control of termites.
Materials and methods
Insects
Six colonies of Odontotermes formosanus were collected from Jurong in Zhenjiang, Jiangsu Province, China. Termites were kept in sealed plastic containers in total darkness at 27 ± 1 °C and 75 ± 1% relative humidity. All colonies were maintained under laboratory conditions without soil and with moist filter paper for one day before the subsequent experiments.
Culture and concentration determination of the S. marcescens strain, SM1
SM1 was isolated from infected O. formosanus and deposited at Nanjing Forestry University, Nanjing. A solid bacterial medium (1 L) was prepared, consisting of 10 g peptone, 20 g beef extract, 2 g NaCl, 2 g K2HPO4, 18 g agar and 1 L H2O with pH 7.2–7.4. A seed medium (1 L) of 10 g peptone, 20 g yeast extract, 2 g NaCl, 2 g K2HPO4 in 1 L of H2O was also prepared. A zymotic medium (1 L) was prepared of 10 g peptone, 30 g soybean oil, 2 g NaCl, 2 g K2HPO4 in I L H2O. As the amount of SM1 increased, its optical density at 600 nm (OD600) also increased. The OD600 value was linearly related to the number of S. marcescens SM1 bacteria and used to determine the bacterial count of the liquid culture. To obtain the concentration of the S. marcescens SM1 suspension, the OD600 value was measured on a spectrophotometer.
Bioassay of components of S. marcescens SM1 fermentation
The concentration of the fermentation medium of S. marcescens SM1 was 1.17 × 1010 cells/ml, and the fermentation medium of S. marcescens SM1 was centrifuged at 5000 r for 20 min at 4 °C. After centrifugation, a surface oil liquid (pigment layer), a middle layer supernatant and a lower layer of bacterial precipitate were obtained. The lower layer of the bacteria precipitate was dissolved by medium. According to the transfer toxicity method (Tang et al. 2007), quality filter paper was first wetted, placed in a 9-cm diameter glass Petri dish, and five O. formosanus worker termites were dipped in each treatment solution. Five soaked termites marked in red on the abdomen and twenty untreated worker termites were placed in the culture dish. The five marked worker termites dipped in the medium and the 20 untreated worker termites were the controls. The termites were observed and recorded in darkness at 25 °C. The experiment group and the control groups were repeated three times in the same manner.
Effect of protease inhibitors on the bioassay of the SM1 fermentation
Bioassay of components of S. marcescens SM1 fermentation showed that the culture supernatant of the bacterial fermentation had a strong toxicity to O. formosanus. Therefore, the supernatant was treated separately with proteinase K at 50 μg/ml, ethylenediaminetetraacetic acid (EDTA) at 10 mmol/L, 1, 10-phenanthroline at 10 mmol/L, and phenylmethanesulfonyl fluoride (PMSF) at 1 m mol/L. There was also a treatment without reagents added and was only heated at 60 °C for 15 min. The toxicity assay was then performed using the transfer toxicity method. The five marked worker termites dipped in the medium and the 20 untreated worker termites were the controls. The experimental groups and the control groups were assayed three times.
Recovery experiment of ZnSO4
After the bioassays using the supernatant treated with different protease inhibitors, the supernatants treated with the two types of protease inhibitors, EDTA and 1,10-phenanthroline, were subjected to zinc sulphate (ZnSO4) recovery treatments. The two supernatants were incubated with ZnSO4 at a concentration of 0.5 mmol/L for 1 h at 22 °C, and the same toxicity assay performed again. The medium incubated at 22 °C for 1 h with a concentration of 0.5 m mol/L ZnSO4 was the control. The concentration of the SM1 fermentation was 1.69 × 1010 cells/ml and the toxicity assay was then performed using the transfer toxicity method. The experimental group and the control group were independently assayed three times.
Statistical analysis
The results were subjected to an analysis of variance (ANOVA) using InStat software (GraphPad, San Diego, CA, USA) with a level of significance at P < 0.05. Tukey’s test was used for multiple comparisons.
Results
Bioassay of components of the SM1 fermentation
The transfer toxicity bioassay against O. formosanus was carried out using the components of the SM1 fermentation. The results show that the toxicity of the components was not different from that of the supernatant (P > 0.05). After 36 h, the corrected mortality rate of the supernatant was approximately 50%, and at 54 h, the rate was close to 80%. After 54 h, toxicity of the lower bacterial layer was relatively poor at only 28.5%. Among the three components, the uppermost oil pigment layer had the poorest toxicity (Table 1).
Effect of protease inhibitors on the bioassay of SM1 fermentation
After the middle layer supernatant was identified as the main active component, it was treated with protease inhibitors. It was first heated at 60 °C for 15 min to denature the protease. The results show that the toxicity effect of the supernatant decreased significantly (P < 0.05) after heating; the toxicity became stronger after 48 h and the corrected mortality reached 60% (P > 0.05). Four different protease inhibitors, PMSF, EDTA, proteinase K and 1, 10-phenanthroline, were added separately to the supernatant. The results show that the toxicity decreased when protease inhibitors were used. When proteinase K was used, the toxicity of the supernatant decreased most, and the corrected mortality rate was 10.4% at 54 h; when EDTA was used, the toxicity of the supernatant was greatly reduced, and the corrected mortality rate was 22.4% at 54 h; when PMSF or 1, 10-phenanthroline was used, the toxicity of the supernatant was reduced, and the corrected mortality rate failed to reach 50% by 54 h (Table 2).
Recovery experiment of ZnSO4
After protease inhibitors suppressed the toxicity of the SM1 fermentation supernatant, it was blocked by two inhibitors of metalloprotease and was subjected to a ZnSO4 recovery experiment. This could confirm that the protease in the supernatant was toxic to O. formosanus and could initially indicate what the active protease was. There was no difference in toxicity between the medium and the medium treated with 0.5 m mol/L ZnSO4, neither of which can caused the death of termites. The EDTA-treated supernatant had an increase in insecticidal activity after the ZnSO4 recovery test (P < 0.05). At 54 h, the corrected mortality increased from 23.6 to 44.4%. In addition, the insecticidal activity of the 1, 10-phenanthroline-treated supernatant was improved after the ZnSO4 recovery experiment, and the corrected mortality at 48 h was significantly different from that before the recovery (P < 0.05). These results further indicate that particular metalloproteases were present in the supernatant, and these metalloproteases were also one of the effective components responsible for the death of O. formosanus (Table 3).
Discussion
Research on biopesticide proteins has been important in the development of biopesticides. Over the past few years, several studies of Bacillus thuringiensis insecticidal protein (Schnepf et al. 1998) have shown that protein crystals produced during sporulation are toxic to many organisms. The expression and structural functions of the protein genes have been studied by McGaughey et al. (1998) and Rajamohan et al. (1998). There are four gene clusters encoding the toxin protein complex in the luminescent bacillus, which makes these bacteria that are symbiotic with entomopathogenic nematodes a member of the microbial insecticide protein family (Bowen et al. 1998). Reports of nematophagous pathogenic bacilli also earlier suggested this symbiosis with entomopathogenic nematodes, and their insecticidal components have a lethal effect on a variety of pests (Forst et al. 1997). Studies have shown that active ingredients that play a major insecticidal role include small molecules (Volgyi et al. 1998), lipopolysaccharides (Smigielski and Akhurst 1995) and proteins. A 1.2 kb gene was obtained from the nematophagous pathogenic bacterium A24, which encodes a 30 kDa insecticidal protein. A few microliters of the fermentation containing the toxic protein can cause the death of insect larvae (Smigielski and Akhurst 1995). Moreover, Clostridium difficile (Barloy et al. 1998) and Bacillus sphaericus (Li et al. 2008) also produce insecticidal proteins which are toxic. Studies have shown that S. marcescens secretes several well-known extracellular proteins, including nuclease, phosphatase, hemolysin, iron-containing proteins, chitinase, protease, and lipase (Tao 2006). One or several of these components may become insecticide factors. Therefore, studying the specific components of S. marcescens SM1 can help to further clarify its mechanism of action on termites.
The uppermost layer of the pigment oil layer, the middle layer supernatant and the bacterial sediment layer were obtained by centrifugation of S. marcescens SM1 fermentation. The results show that the toxicity effect of the supernatant was the best and closest to that of the original fermentation. The toxicity of the bacterial sediment layer was poor and differed from that of the original fermentation. In addition, the pigment oil layer had the least effect, and the mortality rate of O. formosanus treated with the pigment oil layer was very close to that of the controls. Therefore, it was confirmed that the main active component of S. marcescens SM1 fermentation was in the supernatant, and that the pigment oil layer had no insecticidal properties. Since the bacterial precipitate was added to the medium during dissolution, the undestroyed cells might continue to ferment to produce S. marcescens SM1 fermentation and induce a poisoning effect in the subsequent toxicity test experiments.
After identifying that the main active component was the supernatant, it was necessary to further clarify whether the active component was a protease by heating or adding different protease inhibitors. From the results, the toxicity effect of the heated supernatant was significantly reduced, and the corrected mortality rate of the treatment was only 30%. But as time increased, its toxicity gradually increased, which may be caused by the recovery of certain denatured proteases. Since protease K can inhibit most of the proteases, after protease inhibitor K was added, the toxicity effect of the supernatant was greatly reduced, and the mortality of treated O. formosanus was almost the same as that of the control. At the same time, the toxicity effects of the supernatants treated with the three types of protease inhibitors, PMSF, EDTA and 1,10-phenanthroline, were also reduced. At 48 h, the corrected mortality rate of each treatment group was less than 32%. These results indicate that there was more than one insecticidal protease in the supernatant and that these insecticidal proteins were one of the important causes of termite death.
Since both protease inhibitors EDTA and 1,10-phenanthroline inhibit metalloprotease, the supernatants treated with these inhibitors were incubated with 0.5 mmol/L ZnSO4 for 1 h at 22 °C to confirm whether or not the metalloprotease was active. The results show that the insecticidal activity of the supernatant was restored after the ZnSO4 recovery experiment, which indicated that the metalloprotease was present in the supernatant and the insecticidal protease had a good toxicity effect on O. formosanus. However, this experiment only preliminarily confirmed that the active components of S. marcescens SM1 fermentation were metalloproteases and other proteins. More precise active components are not known and further investigation is necessary.
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Project funding: This research was supported by Zhenjiang’s Key R&D Program-Modern Agriculture (NY201702), Jurong Agricultural Science and Technology support plan (NY2017846771) and the Priority Academic Program Development Fund of Jiangsu Higher Education Institutions.
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Fu, R., Luo, J., Feng, K. et al. Termite-killing components in Serratia marcescens (SM1). J. For. Res. 32, 1739–1744 (2021). https://doi.org/10.1007/s11676-020-01172-0
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DOI: https://doi.org/10.1007/s11676-020-01172-0