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Plantlet regeneration through somatic embryogenesis in Nordmann’s fir (Abies nordmanniana)

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Abstract

I studied the influence of various combinations of auxin and cytokinin concentrations, and the increased content of zinc and enzymatic casein hydrolizate in SH medium on initiation and proliferation of embryogenic callus of Abies nordmanniana (Steven) Spach. Additionally, the effect of ABA, PEG-4000 and different wavelengths on the maturation of somatic embryos was tested. The use of optimum composition of modified SH medium with BA, KIN and 2.4-D while simultaneously ensuring appropriate external conditions resulted in 15.5 % embryogenesis. Finally, satisfactory results of micropropagation of A. nordmanniana by somatic embryogenesis were obtained providing seven lines of embryogenic callus with high proliferation capacity. Those lines gave properly developed seedlings in white LED light with a wavelength of 400-700 nm, preceded by eight-week vernalization treatment of the callus. This paper may provide a protocol by which all stages of somatic embryogenesis of A. nordmanniana can be carried out, including the preceding 24-h seed disinfection with NaOCl and PVP, which resulted in 100 % frequency of uninfected zygotic embryos that were capable of starting embryogenesis.

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Abbreviations

BA:

Benzylaminopurine

2,4-D:

2,4-Dichlorophenoxy acetic acid

ESM:

Embryonic suspensor mass

KIN:

Kinetin

PESM:

Pro-embryogenic suspension masses

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Acknowledgments

The author would like to express their special thanks to Professor Tadeusz Kowalski for help in doing microscopic photos.

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Authors and Affiliations

  1. Department of Forest Pathology, Mycology and Tree Physiology, University of Agriculture in Krakow, 29-Listopada Ave 46, 31-425, Kraków, Poland

    Katarzyna Nawrot-Chorabik

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  1. Katarzyna Nawrot-Chorabik
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Corresponding author

Correspondence to Katarzyna Nawrot-Chorabik.

Additional information

Project funding: This work was supported by research topic DS No. 3414 of the Ministry of Science and Higher Education and funded from international corporation Vitroflora.

The online version is available at http://www.springerlink.com

Corresponding editor: Hu Yanbo

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Nawrot-Chorabik, K. Plantlet regeneration through somatic embryogenesis in Nordmann’s fir (Abies nordmanniana). J. For. Res. 27, 1219–1228 (2016). https://doi.org/10.1007/s11676-016-0265-7

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  • DOI: https://doi.org/10.1007/s11676-016-0265-7

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