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Factors affecting mesenchymal stromal cells yield from bone marrow aspiration

  • Original Article
  • Published:
Chinese Journal of Cancer Research

Abstract

Objective

This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells (MSC).

Methods

We determined the yield of MSC from bone marrow under different clinical conditions by comparing the MSC colony numbers from: (1) donors of different ages; (2) healthy donors and patients with leukemia; (3) bone marrow aspirated at different time points during marrow harvesting; (4) bone marrow harvested by different needles.

Results

During the process of harvesting, the number of MSC significantly decreased with increase number of aspiration, from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated, and 50/ml after 200 ml bone marrow aspirated. The number of MSC retrieved from leukemia patients (99/ml bone marrow) was significantly lower than that of healthy donors (708/ml bone marrow). However, there was no significant difference in growth rate. There was no significant age-related difference of MSC yielded from donors <55 years. And there was no significant difference in MSC number between the samples from single end-holed needle and those from multiple-side-hole needle.

Conclusion

The optimal bone marrow samples for MSC collection should be obtained earlier in the process of harvesting procedure. Bone marrow from donors <55 years was equally good as MSC sources. The autologous MSC from leukemia patients can be utilized for in-vitro MSC expansion.

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Authors and Affiliations

  1. China Oxford Centre for International Health Research, Cardiovascular Institute & Fuwai Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100037, China

    Jing Li

  2. Department of Paediatrics and Adolescent Medicine, the University of Hong Kong, HKSAR, Hong Kong, China

    Jing Li, Wilfred Hing-Sang Wong, Shing Chan, Tsz-Leung Lee, Shau-Yin Ha, Yu-Lung Lau & Godfrey Chi-Fung Chan

  3. Department of Medicine, the University of Hong Kong, HKSAR, Hong Kong, China

    James Chor-San Chim, Wing-Yan Au, Albert Kwok-Wei Lie & Raymond Hin-Suen Liang

  4. Department of Orthopaedics & Traumatology, the University of Hong Kong, HKSAR, Hong Kong, China

    Kenneth Man-Chee Cheung

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  1. Jing Li
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  2. Wilfred Hing-Sang Wong
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Correspondence to Godfrey Chi-Fung Chan.

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Li, J., Wong, W.HS., Chan, S. et al. Factors affecting mesenchymal stromal cells yield from bone marrow aspiration. Chin. J. Cancer Res. 23, 43–48 (2011). https://doi.org/10.1007/s11670-011-0043-1

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  • DOI: https://doi.org/10.1007/s11670-011-0043-1

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