Abstract
Titanium dioxide (TiO2) has been shown to exhibit photocatalytic bactericidal activity. This preliminary study focused on examining the photocatalytic activity of high-velocity oxy-fuel (HVOF) sprayed nanostructured TiO2 coatings to kill Pseudomonas aeruginosa. The surfaces of the nanostructured TiO2 coatings were lightly polished before addition of the bacterial solution. Plates of P. aeruginosa were grown, and then suspended in a phosphate buffer saline (PBS) solution. The concentration of bacteria used was determined by a photo-spectrometer, which measured the amount of light absorbed by the bacteria-filled solution. This solution was diluted and pipetted onto the coating, which was exposed to white light in 30-min intervals, up to 120 min. It was found that on polished HVOF-sprayed coatings exposed to white light, 24% of the bacteria were killed after exposure for 120 min. On stainless steel controls, approximately 6% of the bacteria were not recovered. These preliminary results show that thermal-sprayed nanostructured TiO2 coatings exhibited photocatalytic bactericidal activity with P. aeruginosa.
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References
A. Fujishima and K. Honda, Electrochemical Photolysis of Water at a Semiconductor Electrode, Nature, 1972, 238, p 37-38
M. Brunella, M. Diamanti, M. Pedeferri, F. Di Fonzo, C. Casari, and A. Li Bassi, Photocatalytic Behavior of Different Titanium Dioxide Layers, Thin Solid Films, 2007, 515, p 6309-6313
T. Minabe, D. Tryk, P. Sawunyama, Y. Kikuchi, K. Hashimoto, and A. Fujishima, TiO2-Mediated Photodegradation of Liquid and Solid Organic Compounds, J. Photochem. Photobiol. A: Chem., 2000, 137, p 53-62
Y. Kikuchi, K. Sunada, T. Iyoda, K. Hashimoto, and A. Fujishima, Photocatalytic Bactericidal Effect of TiO2 Thin Films: Dynamic View of the Active Oxygen Species Responsible for the Effect, J. Photochem. Photobiol. A: Chem., 1997, 106, p 51-56
P. Amézaga-Madrid, G. Nevarez-Moorillon, E. Orrantia-Borunda, and M. Miki-Yoshida, Photoinduced Bactericidal Activity Against Pseudomonas aeruginosa by TiO2 Based Thin Films, FEMS Microbiol. Lett., 2002, 211, p 183-188
Y. Ohko, K. Hashimoto, and A. Fujishima, Kinetics of Photocatalytic Reactions Under Extremely Low-Intensity UV Illumination on Titanium Dioxide Thin Films, J. Phys. Chem. A, 1997, 101, p 8057-8062
L. Brook, P. Evans, H. Foster, M. Pemble, A. Steele, D. Sheel, and H. Yates, Highly Bioactive Silver and Silver/Titania Composite Films Grown by Chemical Vapour Deposition, J. Photochem. Photobiol. A: Chem., 2007, 187, p 53-63
R.S. Lima and B.R. Marple, From APS to HVOF Spraying of Conventional and Nanostructured Titania Feedstock Powders: A Study on the Enhancement of the Mechanical Properties, Surf. Coat. Technol., 2006, 200, p 3428-3437
G. Yang, C. Li, F. Han, and A. Ohmori, Microstructure and Photocatalytic Performance of High Velocity Oxy-Fuel Sprayed TiO2 Coatings, Thin Solid Films, 2004, 466, p 81-85
G. Bodey, R. Bolivar, V. Fainstein, and L. Jadeja, Infections Caused by Pseudomonas aeruginosa, Rev. Infect. Dis, 1983, 5, p 279-313
J. Costerton, Cystic Fibrosis Pathogenesis and the Role of Biofilms in Persistent Infections, Trends Microbiol., 2001, 9, p 50-52
E. VanHaecke, J. Remon, M. Moors, F. Raes, D. de Rudder, and A. Van Peteghem, Kinetics of Pseudomonas aeruginosa Adhesion to 304 and 316-L Stainless Steel: Role of Cell Surface Hydrophobicity, Appl. Environ. Microbiol., 1990, 56, p 788-795
C. Johansen, P. Falholt, and L. Gram, Enzymatic Removal and Disinfection of Bacterial Biofilms, Appl. Environ. Microbiol., 1997, 63, p 3724-3728
C. Giltner, E. van Schaik, G. Audette, D. Kao, R. Hodges, D. Hassett, and R. Irvin, The Pseudomonas aeruginosa Type IV Pilin Receptor Binding Domain Functions as an Adhesin for Both Biotic and Abiotic Surfaces, Mol. Microbiol., 2006, 59, p 1083-1096
J. Taylor, An Introduction to Error Analysis: The Study of Uncertainties in Physical Measurements, University Science Books, New York, NY, 1982
C. Ryder, M. Byrd, and D. Wozniak, Role of Polysaccharides in Pseudomonas aeruginosa Biofilm Development, Curr. Opin. Microbiol., 2007, 10, p 644-648
N. Berger-Keller, G. Bertrand, C. Filiare, C. Meunier, and C. Coddet, Microstructure of Plasma-Sprayed Titania Coatings Deposited from Spray-Dried Powder, Surf. Coat. Technol., 2007, 168, p 281-290
Acknowledgments
The authors gratefully acknowledge the assistance of Linh Lu with collection of data for the samples exposed to short wavelength UV illumination. Funding for this project was provided by the Natural Sciences and Engineering Research Council of Canada (NSERC), the Government of Alberta Small Equipment Grants Program (SEGP), the Canada Foundation for Innovation (CFI), and the National Research Council of Canada (NRC).
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This article is an invited paper selected from presentations at the 2009 International Thermal Spray Conference and has been expanded from the original presentation. It is simultaneously published in Expanding Thermal Spray Performance to New Markets and Applications: Proceedings of the 2009 International Thermal Spray Conference, Las Vegas, Nevada, USA, May 4-7, 2009, Basil R. Marple, Margaret M. Hyland, Yuk-Chiu Lau, Chang-Jiu Li, Rogerio S. Lima, and Ghislain Montavon, Ed., ASM International, Materials Park, OH, 2009.
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Jeffery, B., Peppler, M., Lima, R.S. et al. Bactericidal Effects of HVOF-Sprayed Nanostructured TiO2 on Pseudomonas aeruginosa . J Therm Spray Tech 19, 344–349 (2010). https://doi.org/10.1007/s11666-009-9369-3
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DOI: https://doi.org/10.1007/s11666-009-9369-3