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Cytotoxicity of Ni from Surface-Treated Porous Nitinol (PNT) on Osteoblast Cells

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Abstract

The leaching of nickel from the surface of porous Nitinol (PNT) is mainly dependent on its surface characteristics, which can be controlled by appropriate surface treatments. In this investigation, PNT was subjected to two surface treatments, namely, water-boiling and dry-heating passivations. Phosphate buffer saline (PBS) solutions obtained from cyclic potentiodynamic polarization tests on PNT were employed to assess the cytotoxicity of Ni contained therein on osteoblast cells by Sulforhodamine B (SRB) assay. In addition, similar concentrations of Ni were added exogenously to cell culture media to determine cytotoxic effects on osteoblast cells. The morphologies of the untreated and the surface-treated PNTs were examined using SEM and AFM. Furthermore, growth of human osteoblast cells was observed on the PNT surfaces.

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Acknowledgment

This research was supported by the Award number SC3GM084816 from the National Institute of General Medical Science.

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Authors and Affiliations

  1. Applied Research Center, Florida International University, Miami, FL, USA

    C. Pulletikurthi, N. Munroe, P. Gill, S. Pandya, D. Persaud, W. Haider, K. Iyer & A. McGoron

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  1. C. Pulletikurthi
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  2. N. Munroe
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  3. P. Gill
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Correspondence to C. Pulletikurthi.

Additional information

This article is an invited paper selected from presentations at Shape Memory and Superelastic Technologies 2010, held May 16-20, 2010, in Pacific Grove, California, and has been expanded from the original presentation.

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Pulletikurthi, C., Munroe, N., Gill, P. et al. Cytotoxicity of Ni from Surface-Treated Porous Nitinol (PNT) on Osteoblast Cells. J. of Materi Eng and Perform 20, 824–829 (2011). https://doi.org/10.1007/s11665-011-9930-3

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  • DOI: https://doi.org/10.1007/s11665-011-9930-3

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