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Label-Free Quantitative Immunoassay of Fibrinogen in Alzheimer Disease Patient Plasma Using Fiber Optical Surface Plasmon Resonance

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Abstract

We present a real-time quantitative immunoassay to detect fibrinogen in the blood plasma of Alzheimer’s disease patients using multimode fiber optical sensors in which surface plasmon resonance (SPR) was employed. Nanometer-thick bimetals including silver and aluminum were coated onto the core surface of the clad-free part (5 cm long) of the fiber for SPR excitation at the He-Ne laser wavelength of 632.8 nm. The histidine-tagged peptide was then coated on the metal surface to immobilize the fibrinogen antibody for the selective capture of fibrinogen among the proteins in the patient blood plasma. The SPR fiber optical sensor enabled quantitative detection of concentrations of fibrinogen from the different human patient blood at a detection limit of ∼20 ng/ml. We also observed a correlation in the fibrinogen concentration measurement between enzyme-linked immunosorbent assay and our SPR fiber-based sensors. This suggests that the presented SPR fiber-based sensors that do not rely on the use of labels such as fluorophores can be used for a real-time quantitative assay of a specific protein such as fibrinogen in a human blood that is known to contain many other kinds of proteins together.

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Acknowledgements

This work was supported by the Gachon University research fund of 2014 (GCU-2014-0157), and also by the Industrial Core Technology Development Program (Grant No. 10049051, Development of bench-top automatic immunoassay system with intelligent quality control features for screening cancer or chronic diseases in local clinical setting) funded By the Ministry of Trade, industry & Energy (MI, Korea).

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Authors and Affiliations

  1. Department of Nano-Physics, College of BioNano Technology, Gachon University, Seongnam, 461-701, Republic of Korea

    Jisoo Kim & Heongkyu Ju

  2. Department of BioNano Technology, College of BioNano Technology, Gachon University, Seongnam, 461-701, Republic of Korea

    SeJin Kim, Tan Tai Nguyen, Seong Soo A. An & Heongkyu Ju

  3. Seoul International School, Seongnam-daero 1518 beon-gil, Sujeong-gu, Seongnam-si, Gyeonggi-do, 13113, Republic of Korea

    Renee Lee

  4. Hwasung High School, Jangjim-ri 161, Hyangnam-eup, Hwaseong-si, Gyeonggi-do, 455-925, Republic of Korea

    Tiehua Li, Changhyun Yun & Youngeun Ham

  5. Neuroscience Institute, Gil Hospital, Incheon, 405-760, Republic of Korea

    Heongkyu Ju

  6. Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Bokjeong-dong, Sujeong-gu, Seongnam-si, Gyeonggi-do, 461-701, Republic of Korea

    Seong Soo A. An

  7. Department of Nano-Physics, Gachon Medical Research Institute, Gachon University, Bokjeong-dong, Sujeong-gu, Seongnam-si, Gyeonggi-do, 461-701, Republic of Korea

    Heongkyu Ju

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  1. Jisoo Kim
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  2. SeJin Kim
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  3. Tan Tai Nguyen
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  4. Renee Lee
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  5. Tiehua Li
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  6. Changhyun Yun
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  7. Youngeun Ham
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  8. Seong Soo A. An
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  9. Heongkyu Ju
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Corresponding authors

Correspondence to Seong Soo A. An or Heongkyu Ju.

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Kim, J., Kim, S., Nguyen, T. . et al. Label-Free Quantitative Immunoassay of Fibrinogen in Alzheimer Disease Patient Plasma Using Fiber Optical Surface Plasmon Resonance. J. Electron. Mater. 45, 2354–2360 (2016). https://doi.org/10.1007/s11664-015-4292-5

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  • DOI: https://doi.org/10.1007/s11664-015-4292-5

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