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Pulmonoprotective Action of Zataria multiflora Ethanolic Extract on Cyclophosphamide-Induced Oxidative Lung Toxicity in Mice

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Abstract

Objective

To evaluate the protective effect of Zataria multiflora extract, an antioxidative medicinal plant, against cyclophosphamide (CP)-induced oxidative lung damage in mice.

Methods

Mice were intraperitoneally pre-treated with various doses of Zataria multiflora extract (50, 100, 200, and 400 mg/kg) once daily for 7 consecutive days. Animals were then injected with a single 200 mg/kg intraperitoneal dose of CP 1 h after the last administration of O. vulgare. Twenty-four hours later, mice were euthanized, the lungs were immediately removed, and biochemical and histological studies were conducted.

Results

A single dose of CP markedly altered the levels of several biomarkers associated with oxidative stress in lung homogenates. Pretreatment with Zataria multiflora significantly inhibited the elevation of lipid peroxidation level and the depletion in glutathione content, and superoxide dismutase and catalase activities induced by CP in lung. In addition, Zataria multiflora effectively alleviated CP-induced histopathological abnormality and pulmonary damages in mice lung tissues.

Conclusions

The results reveal that Zataria multiflora protects lung tissues from CP-induced toxicity and suggest a role for oxidative stress in the pathogenesis of lung toxicity produced by CP in mice. Because Zataria multiflora has been extensively used as an additive agent and is regarded as safe, it may be used concomitantly as a good supplement for reducing organ toxicity in patients undergoing chemotherapy, besides their consolidated ethnopharmacological uses.

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Authors and Affiliations

  1. Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, 48175-861, Iran

    Emran Habibi, Mohammad Shokrzadeh & Amirhossein Ahmadi

  2. Department of Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, 48175-861, Iran

    Emran Habibi

  3. Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, 48175-861, Iran

    Mohammad Shokrzadeh & Hamed Haghi-Aminjan

  4. Student Research Committee, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, 48175-861, Iran

    Aroona Chabra

  5. Department of Pathology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, 48471-91971, Iran

    Farshad Naghshvar & Fatemeh Salehi

Authors
  1. Emran Habibi
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  2. Mohammad Shokrzadeh
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  3. Amirhossein Ahmadi
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  4. Aroona Chabra
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  5. Farshad Naghshvar
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  6. Hamed Haghi-Aminjan
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  7. Fatemeh Salehi
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Correspondence to Amirhossein Ahmadi.

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Supported by the Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran (No. 92.60-92.90)

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Habibi, E., Shokrzadeh, M., Ahmadi, A. et al. Pulmonoprotective Action of Zataria multiflora Ethanolic Extract on Cyclophosphamide-Induced Oxidative Lung Toxicity in Mice. Chin. J. Integr. Med. 26, 754–761 (2020). https://doi.org/10.1007/s11655-018-2984-4

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