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Lignans-rich extract from Herpetospermum caudigerum alleviate physical fatigue in mice

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Abstract

Objective

To ascertain anti-fatigue constituents and mechanisms of Herpetospermum caudigerum.

Methods

The 80% ethanol extracts of Herpetospermum caudigerum were partitioned with chloroform, ethyl acetate and n-butanol, respectively. Male Kunming mice were divided into 13 groups with 16 mice in each group: a control group fed with water, 9 groups treated with 3 fractions of Herpetospermum caudigerum (chloroform fraction, ethyl acetate fraction and n-butanol fraction) at dose of 80, 160 and 320 mg/kg for the low-dose group, medium-dose group and high-dose group, 3 herpetrione (HPE) treated groups fed with HPE at dose of 15, 30, and 60 mg/kg for the low-dose group, medium-dose group and high-dose group. All animals were treated once per day for 30 days. Anti-fatigue activity was assessed through the forced swimming test and serum biochemical parameters including blood lactic acid (BLA), blood urea nitrogen (BUN), malondialdehyde (MDA), hepatic glycogen (HG), lactic dehydrogenase (LDH), superoxide dismutase (SOD) and glutathione peroxidase (GPx) determined following the recommended procedures provided by the commercial kits.

Results

Compared with the control group, the lignans extract (ethyl acetate fraction) of Herpetospermum caudigerum and HPE could signifificantly prolonged the exhaustive swimming time (P<0.05 or P<0.01), and also increased the HG levels (P<0.05 or P<0.01) and the activities of antioxidant enzymes (SOD, GPx and LDH, P<0.05 or P<0.01); BLA and MDA levels were decreased considerably in lignans extract and HPE treated groups (P<0.05 or P<0.01). HPE also could significantly decrease the BUN contents compared with the control group (P<0.05). The chloroform and n-butanol fraction showed no effect on swimming time and biochemical parameters.

Conclusions

The lignans extract had antifatigue activities and HPE may be partly responsible for the anti-fatigue effects of Herpetospermum caudigerum. The possible mechanisms of anti-fatigue activity were related to the decrease of BUN and BLA, the increase of the HG storage and protecting corpuscular membrane by preventing lipid oxidation via modifying several enzyme activities.

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Authors and Affiliations

  1. Department of Pharmacy, 302 Hospital of People’s Liberation Army, Beijing, 100039, China

    Shi-ying Jin  (靳世英), Bao-de Shen  (申宝德), Jin-xia Bai  (白金霞), Ping-hua Xu  (徐平华), Ling Dai  (戴 领), He Xu  (徐 和), Shi-xiao Jin  (靳士晓), Jin Han  (韩 晋) & Hai-long Yuan  (袁海龙)

  2. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China

    Shi-ying Jin  (靳世英), Jin-xia Bai  (白金霞), Ling Dai  (戴 领), He Xu  (徐 和) & Shi-xiao Jin  (靳士晓)

  3. Animal Laboratory Center, 302 Hospital of People’s Liberation Army, Beijing, 100039, China

    Rui-sheng Li  (李瑞生)

  4. Key Lab of Modern Preparation of Traditimal Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, China

    Bao-de Shen  (申宝德)

Authors
  1. Shi-ying Jin  (靳世英)
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  2. Rui-sheng Li  (李瑞生)
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  3. Bao-de Shen  (申宝德)
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  4. Jin-xia Bai  (白金霞)
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  5. Ping-hua Xu  (徐平华)
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  6. Ling Dai  (戴 领)
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  7. He Xu  (徐 和)
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  8. Shi-xiao Jin  (靳士晓)
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  9. Jin Han  (韩 晋)
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  10. Hai-long Yuan  (袁海龙)
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Corresponding author

Correspondence to Hai-long Yuan  (袁海龙).

Additional information

Supported by the National Key New Drugs Innovation Foundation (No. 2009ZX09103-349) and the Beijing Natural Science Foundation (No. 7122176)

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Jin, Sy., Li, Rs., Shen, Bd. et al. Lignans-rich extract from Herpetospermum caudigerum alleviate physical fatigue in mice. Chin. J. Integr. Med. 22, 840–845 (2016). https://doi.org/10.1007/s11655-016-2254-2

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  • DOI: https://doi.org/10.1007/s11655-016-2254-2

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