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Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior and glial fibrillary acidic protein expression in cerebral hippocampus of a rat vascular depression model

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Abstract

Objective

To explore the effects and anti-depression mechanisms of Kaixin Jieyu Decoction (开心解郁汤, KJD).

Methods

The rat vascular depression (VD) model was established by ligation of bilateral common carotid arteries (LBCCA) combined with chronic unpredictable mild stress (CUMS). Forty Wistar rats were randomly divided into sham, VD model, VD + high-dose KJD [15.4 g/(kg·d) of crude drug], VD + medium-dose KJD [7.7 g/(kg·d) of crude drug], and VD + fluoxetine [2.4 mg/(kg·d)] groups (n=8 in each group), and the treatments lasted for 21 days. Changes of behavior and hippocampus pathology were observed. The level of glial fibrillary acidic protein (GFAP) protein and mRNA in hippocampus was detected respectively by immunohistochemistry and real-time polymerase chain reaction.

Results

Compared with the sham group, rats in model group showed a variety of behavioral obstacles, including a significant reduction in sucrose consumption percentage, horizontal and vertical activity scores in open-field tests (P<0.05 or P<0.01), pathological damage like neuronal degeneration, necrosis, and a significant decrease of GFAP protein and mRNA in hippocampus (P<0.01); compared with the model group, rats in the high-dose KJD group, medium-dose KJD group and fluoxetine group obtained notable higher behavioral scores, and pathological injury lessened in hippocampus with a increased expression of GFAP protein and mRNA P<0.05 or P<0.01); compared with the medium-dose KJD group and fluoxetine group, GFAP mRNA in high-dose KJD group expressed higer (P<0.05).

Conclusion

LBCCA combined with CUMS may cause depression-like behavioral changes resulting in the VD model of rats whose depression state can be ameliorated by KJD, and the mechanism of cerebral protection is related possibly with promoting expression of GFAP in hippocampus.

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Authors and Affiliations

  1. Traditional Chinese Medicine Research and Development Center, Guang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, 100053, China

    Xian-hui Zhang  (张先慧), Shi-jing Huang  (黄世敬), Yan-yun Wang  (王彦云), Ying Zhang  (张 颖), Ju-hua Pan  (潘菊华), Jun Zheng  (郑 军), Duo-jiao Li  (李多娇) & Xiao-ming Lei  (雷小明)

Authors
  1. Xian-hui Zhang  (张先慧)
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  2. Shi-jing Huang  (黄世敬)
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  3. Yan-yun Wang  (王彦云)
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  4. Ying Zhang  (张 颖)
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  5. Ju-hua Pan  (潘菊华)
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  6. Jun Zheng  (郑 军)
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  7. Duo-jiao Li  (李多娇)
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  8. Xiao-ming Lei  (雷小明)
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Corresponding author

Correspondence to Shi-jing Huang  (黄世敬).

Additional information

Supported by the National Natural Science Foundation of China (No. 81072801 and 30672696)

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Zhang, Xh., Huang, Sj., Wang, Yy. et al. Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior and glial fibrillary acidic protein expression in cerebral hippocampus of a rat vascular depression model. Chin. J. Integr. Med. 21, 223–228 (2015). https://doi.org/10.1007/s11655-014-1820-8

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  • DOI: https://doi.org/10.1007/s11655-014-1820-8

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