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Effects of Modified Sanhuang Decoction (加味三黄汤) enema on serum tumor necrosis factor-α and colonic mucosa interleukin-1β, interleukin-6 levels in ulcerative colitis rats

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Abstract

Objective

To investigate the effects of Modified Sanhuang Decoction (加味三黄汤, MSD) enema on the serum tumor necrosis factor alpha (TNF-α) and colonic mucosa interleukin-1β (IL-1β), interleukin-6 (IL-6) levels in experimental ulcerative colitis (UC) rats.

Methods

Forty-five male Wistar rats were randomly divided into 4 groups: normal group (n=12), model group (n=11), salazosulfapyridine (SASP) group (n=11) and MSD group (n=11). The UC model was induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS)/ethanol solution. Rats in the normal group and model group were clystered with 0.9% normal saline, while in the SASP group and MSD group were clystered with SASP and MSD enema, respectively. After drug administration (10 mL/kg body weight, for 7 days), colonic gross changes and colonic mucosa histology were observed, serum TNF-α and colonic mucosa IL-1β, IL-6 levels were tested by enzyme linked immunosorbent assay and radioimmunoassay, respectively.

Results

As compared with the normal group, the experimental UC rats, the colonic mucosal damage index scores (CMDIs), histopathological scores (HS) and the serum TNF-α and colonic mucosa IL-1β, IL-6 levels significantly increased (P<0.05 or P<0.01). In the MSD and SASP groups, the ulcer area significantly reduced, and edema disappeared. The CMDIs, HS, the serum TNF-α and colonic mucosa IL-1β, IL-6 levels in the MSD and SASP groups significantly decreased (P<0.05 or P<0.01) compared with the model group. The CMDIs in the MSD group were lower than that in the SASP group (P<0.05), but there were no significant differences in HS, serum TNF-α or colonic mucosa IL-1β, IL-6 levels between the MSD and SASP groups.

Conclusion

MSD enema can improve colonic mucosa impairment and decrease serum TNF-α and colonic mucosa IL-1β, IL-6 levels in experimental UC.

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Authors and Affiliations

  1. Beijing Traditional Chinese Medicine Hospital Affiliated to Capital Medical University, Beijing, 100010, China

    Shuai Wang  (王 帅), Tao Zhou  (周 滔), Jun-peng Zhai  (翟军鹏) & Jing Chen  (陈 誩)

  2. Beijing Institute of Traditional Chinese Medicine, Beijing, 100010, China

    Li-hua Wang  (王丽华)

Authors
  1. Shuai Wang  (王 帅)
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  2. Tao Zhou  (周 滔)
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  3. Jun-peng Zhai  (翟军鹏)
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  4. Li-hua Wang  (王丽华)
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  5. Jing Chen  (陈 誩)
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Corresponding author

Correspondence to Jing Chen  (陈 誩).

Additional information

Supported by Beijing Excellent Talents Cultivation Project (No. 2010D003034000044)

These authors contributed equally to this work

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Wang, S., Zhou, T., Zhai, Jp. et al. Effects of Modified Sanhuang Decoction (加味三黄汤) enema on serum tumor necrosis factor-α and colonic mucosa interleukin-1β, interleukin-6 levels in ulcerative colitis rats. Chin. J. Integr. Med. 20, 865–869 (2014). https://doi.org/10.1007/s11655-013-1538-z

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  • DOI: https://doi.org/10.1007/s11655-013-1538-z

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