Abstract
Objective
To investigate the role of Shenfu Injection (参附注射液, SFI) in rats with systemic inflammatory response syndrome (SIRS).
Methods
The SIRS rat model was induced by the intravenous injection of lipopolysaccharide (LPS). Forty-five male Wistar rats were randomly divided into 3 groups, the sham operative control group (control group, n=5), the SIRS model group (model group, n=20) and the SFI treatment group (SFI group, n=20). LPS was injected through the external jugular vein (12 mg/kg, 6 mg/mL) to all rats except for those in the control group, and SFI (10 mL/kg) was given to those in the SF group only once through intraperitoneal injection, while the normal saline (10 mL/kg) was given to those in the model group. For those in the control group, normal saline was given through the external jugular vein (2 mL/kg) and intraperitoneal injection (10 mL/kg). Then, rats in the model group and SFI group were divided into 4 subgroups according to the time points, i.e., 1 h, 2 h, 4 h and 6 h subgroups, 5 rats in each group. The activity of nuclear factor of κ B (NF-κ B) of in blood mononuclear cells and the plasma levels of tumor necrosis factor-α (TNF-α) and interleukin 6-(IL-6) were determined using enzyme-linked immunoabsordent assay (ELISA) at 1 h, 2 h, 4 h and 6 h after modeling. Histopathologic changes of the lung and liver were observed under a light microscope.
Results
Compared with the control group, the activity of NF-κ B in mononuclear cells and the plasma level of TNF-α were obviously increased at each time points (all P<0.01), reaching the peaks at 2 h after modeling. The plasma level of IL-6 increased gradually as time went by in the model group (P<0.01). Pathological examination showed pulmonary alveoli hemorrhage, edema and inflammatory cell infiltration in the lung tissue, and angiotelectasis, congestion, and local necrosis in the liver tissue in the model group. Compared with the model group, the activity of NF-κ B and the levels of TNF-α and IL-6 in plasma decreased significantly in the SFI group (P<0.01), and the pathological injury in the lungs and liver was significantly alleviated.
Conclusion
SFI plays a protective role by inhibiting the activity of NF-κB, and reducing the expressions of TNF-α and IL-6 in SIRS rats.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Christman JW, Lancaster LH, Blackwell TS. Nuclear factor κ B: a pivotal role in the systemic inflammatory response syndrome and new target for therapy. Intens Care Med 1998;24:1131–1138.
Abraham E. Nuclear factor-κ B and its role in sepsisassociated organ failure. Infect Dis 2003;187(Suppl 2): S364–S369.
Taniguchi T, Kanakura H, Yamamoto K. Effects of posttreatment with propofol on mortality and cytokine responses to endotoxin-induced shock in rats. Crit Care Med 2002;30:904–907.
de Rossi LW, Brueckmann M, Rex S, et al. Xenon and isoflurane differentially modulate lipopolysaccharideinduced activation of the nuclear transcription factor κB and production of tumor necrosis factor-alpha and interleukin-6 in monocytes. Anesth Analg 2004;98:1007–1012.
Hu S, Sheng ZY, Zhou BT. The advance of MODS animal model research. Chin Crit Care Med (Chin) 1999;11(8):504–507.
Taniguchi T, Kidani Y, Kanakura H, et al. Effects of dexmedetomidine on mortality rate and inflammatory responses to endotoxin-induced shock in rats. Crit Care Med 2004;32:1322–1326.
Bohrer H, Qiu F, Zimmermann T, et al. Role of NF-κB in mortality of sepsis. J Clin Invest 1997;100:972–985.
Paterson RL, Galley HF, Dhillon JK, et al. Increased nuclear factor κB activation in critically ill patients who die. Crit Care Med 2002;28:1047–1051.
Blackwell TS, Christman JW. The role of nuclear κB in cytokine gene regulation. Am J Respir Cell Mol Biol 1997;17:3–9.
Kim PK, Deutschman CS. Inflammatory responses and mediators. Surg Clin North Am 2000;80:885–894.
Xiao L, Wang ZQ. The development of cytokines in infect shock. Foreign Med Sci: Section Pathophysiol Clin Med (Chin) 2003;23(1):100–102.
Hu G, Liu XY, Xia ZY, et al. Effect of Shen-Fu Injection on expression of nuclear factor-κB, ICAM-1, iNOS and TNF-α after ischemia-reperfusion of intestinal mucosa in rats. J Tongji Univ (Med Sci, Chin) 2003;24(5):381–384.
Author information
Authors and Affiliations
Corresponding author
Additional information
Supported by a Grant from Hubei Province Science and Technique Foundation (No. 2003AA301C51)
Rights and permissions
About this article
Cite this article
Wang, J., Qiao, Lf. & Yang, Gt. Role of Shenfu Injection (参附注射液) in rats with systemic inflammatory response syndrome. Chin. J. Integr. Med. 14, 51–55 (2008). https://doi.org/10.1007/s11655-008-0051-2
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11655-008-0051-2