Abstract
The effect of NaCl on growth, biomass and ion relations of two salt-tolerant isolates of Paxillus involutus, MAJ and NAU were investigated. The two Paxillus strains were exposed to the following concentrations of NaCl: 0, 100, 200 and 500 mmol·L−1. Growth of MAJ and NAU was enhanced by 100 mmol·L−1 NaCl but severely inhibited at the concentration of 500 mmol·L−1. NAU exhibited a greater capacity to exclude Na+ and Cl− under all salinity levels, whereas the salt-includer MAJ had a higher capacity in nutrient uptake under salt stress. The ratios Na+/K+, Na+/Ca2+ and Na+/Mg2+ in NaCl-treated MAJ and NAU did not increase at levels of 100 and 200 mmol·L−1 NaCl but markedly increased at 500 mmol·L−1. This suggests that the two strains, especially MAJ, enhanced nutrient uptake corresponding to the increased Na+ influx at moderate salinity. We conclude that both MAJ and NAU are able to tolerate 500 mmol·L−1 NaCl but there are species-specific differences in retaining ionic homeostasis in the two Paxillus strains. NAU is a salt-excluder, MAJ is a salt-includer but retains a high capacity in nutrient selectivity under saline conditions. Their definite mechanisms to enhance salt tolerance of mycorrhizal hosts need further study.
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Baum C, Schimd K, Makeschin F. 2000. Interactive effects of substrates and ectomycorrhizal colonization on growth of a poplar clone. Plant Nutr Soil Sci, 163: 221–226
Blaudez D, Botton B, Chalot M. 2000. Cadmium uptake and subcellular compartmentation in the ectomycorrhizal fungus Paxillus involutus. Microbiology, 146: 1,109–1,117
Blomberg A, Adler L. 1993. Tolerance of fungi to NaCl. In: Tennings D H (ed.), Stress Tolerance of Fungi. New York: Marcel Dekker, Inc., 209–232
Bücking H, Arnd J, Kuhn W, Schröder H, Heyser W. 2002. The fungal sheath of ectomycorrhizal pine roots: an apoplastic barrier for the entry of calcium, magnesium, and potassium into the root cortex? Exp Bot, 53(374): 1,659–1,669
Chen D M, Ellul S, Herdman K, Cairney J W C. 2001a. Influence of salinity on biomass production by Australian Pisolithus spp. isolates. Mycorrhiza, 11: 231–236
Chen S L, Li J K, Fritz E, Wang S S, Hüttermann A. 2002. Sodium and chloride distribution in roots and transport in three poplar genotypes under increasing NaCl stress. For Ecol Manage, 168: 217–230
Chen S L, Li J K, Wang S S, Hüttermann A, Altman A. 2001b. Salt, nutrient uptake and transport, and ABA of Populus euphratica; a hybrid in response to increasing soil NaCl. Trees, 15(3): 186–194
Chen S L, Li J K, Wang S S, Fritz E, Hüttermann A, Altman A. 2003. Effects of NaCl on shoot growth, transpiration, ion compartmentation and transport in regenerated plants of Populus euphratica and Populus tomentosa. Can J For Res, 33: 967–975
Gafur A, Schützendübel A, Langenfeld-Heyser R, Fritz E, Polle A. 2003. Compatible and incompetent Paxillus involutus isolates for ectomycorrhiza formation in vitro with poplar (Populus × canescens) differ in H2O2 production. Plant Biol, 6: 91–99
Högberg M N, Högberg P. 2002. Extramatrical ectomycorrhizal mycelium contributes half the microbial biomass and produces, together with associated roots, half the dissolved organic carbon in a forest soil. New Phytol, 154: 791–795
Högberg P, Nordgren A, Buchmann N, Taylor A F S, Ekblad A, Hogberg M N, Nyberg G, Ottosson-Lofvenius M, Read D J. 2001. Large-scale forest girdling shows that current photosynthesis drives soil respiration. Nature, 411: 789–792
Kernaghan G, Hambling B, Fung M. 2002. In vitro selection of boreal ectomycorrhizal fungi for use in reclamation of saline-alkaline habitats. Res Ecol, 10(1): 43–51
Langenfeld-Heyser R, Gao J, Ducic T, Tachd Ph, Lu C, Fritz E, Gafur A, Polle A. 2007. Paxillus involutus mycorrhiza attenuate NaCl-stress responses in the salt-sensitive hybrid poplar Populus × canescens. Mycorrhiza, 17: 121–131
LeQuéré A, Johansson T, Tunlid A. 2002. Size and complexity of the nuclear genome of the ectomycorrhizal fungus Paxillus involutus. Fung Genet Biol, 36: 234–241
Leyval C, Turnau K, Haselwandter K. 1997. Effect of heavy metal pollution on mycorrhizal colonization and function: physiological, ecological and applied aspects. Mycorrhiza, 7: 139–153
Muhsin T M, Zwiazek J J. 2002. Colonization with Hebeloma crustuliniforme increases water conductance and limits shoot sodium uptake in white spruce (Picea glauca) seedlings. Plant Soil, 238: 217–225
Nagarajan G, Natarajan K. 1999. The use of Box-Behnken design of experiments to study in vitro salt tolerance by Pisolithus tinctorius. World J Microbiol Biotech, 15(2): 197–203
Pella E. 1990. Elemental organic analysis, part 2: State of the art. Am Lab, 22–28
Rabie G H, Almadini A M. 2005. Role of bioinoculants in development of salt-tolerance of Vicia faba plants under salinity stress. Afric J Biotech, 4(3): 210–222
Rao A V, Tak R. 2002. Growth of different tree species and their nutrient uptake in limestone mine spoil as influenced by arbuscular mycorrhizal (AM) fungi in India arid zone. J Arid Environ, 51: 113–119
Scheloske S, Maetz M, Schneider T, Hildebrandt U, Bothe H, Povh B. 2004. Element distribution in mycorrhizal and nonmycorrhizal roots of the halophyte Aster tripolium determined by proton induced X-ray emission. Protoplasma, 223: 183–189
Shaw G, A Leake J M, Read D J. 1990. The biology of mycorrhizal in the Ecricaceae (XVII). The role of mycorrhizal infection in the regulation of iron uptake by ericaceous plants. New Phytol, 115: 251–258
Söderström B. 2002. Challenges for mycorrhizal research into the new millennium. Plant Soil, 244: 1–7
Storey R. 1995. Salt tolerance, ion relations and the effects of root medium on the response of citrus to salinity. Plant Physiol, 22: 101–114
Tibbett M, Sanders F E. 2002. Ectomycorrhizal symbiosis can enhance plant nutrition through improved access to discrete organic nutrition patches of high resource quality. Ann Bot, 89: 783–789
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ZHANG Hua and LI Jing contributed equally to this work.
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Zhang, H., Li, J., Chen, Sl. et al. Effect of NaCl on growth and ion relations in two salt-tolerant strains of Paxillus involutus . For. Stud. China 10, 95–100 (2008). https://doi.org/10.1007/s11632-008-0025-7
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DOI: https://doi.org/10.1007/s11632-008-0025-7