Summary
Hairy root cultures of Artemisia annua L. were cultivated in three different mist bioreactors, each fitted with three stainless steel meshes. The growth rates in the three 2.3-L mist bioreactors differed. After 25 d, the growth index (final dry weight/initial dry weight) of the roots was 42 in a nutrient mist bioreactor, 61 in an inner-loop nutrient mist bioreactor, and 68 in a modified inner-loop nutrient mist bioreactor. Under a misting cycle of 3/30 (ON 3 min/OFF 30 min) for 25 d, dry weight reached 13.6 g/L of medium in the modified inner-loop nutrient mist bioreactor in which nutrient could be supplied without dilution of mist by air flow.
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Liu, C.Z., Wang, Y.C., Zhao, B. et al. Development of a nutrient mist bioreactor for growth of hairy roots. In Vitro Cell.Dev.Biol.-Plant 35, 271–274 (1999). https://doi.org/10.1007/s11627-999-0091-0
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DOI: https://doi.org/10.1007/s11627-999-0091-0